3.4.24.25: vibriolysin
This is an abbreviated version!
For detailed information about vibriolysin, go to the full flat file.
Word Map on EC 3.4.24.25
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3.4.24.25
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3.4.24.4
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thermolysin
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cholerae
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thermoproteolyticus
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metalloendopeptidase
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proteolyticus
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thermolysin-like
- 3.4.24.25
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3.4.24.4
- thermolysin
- cholerae
- thermoproteolyticus
- metalloendopeptidase
- proteolyticus
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thermolysin-like
Reaction
Preferential cleavage of bonds with bulky hydrophobic groups in P2 and P1'. Phe at P1' is the most favoured residue, which distinguished this enzyme from thermolysin =
Synonyms
Aeromonas neutral protease, Aeromonas proteolytica neutral proteinase, Aeromonolysin, E495, EC 3.4.24.4, HA/protease, hAPA, hemagglutinin/protease HA/P, MCP-02, MvP1, NprV-R, Proteinase, Aeromonas proteolytica neutral, Vibriolysin
ECTree
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Substrates Products
Substrates Products on EC 3.4.24.25 - vibriolysin
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REACTION DIAGRAM
HlyA protein + H2O
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the enzyme degrades HlyA to diminish Vibrio cholerae hemolytic activity
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furylacryloyl-Gly + Leu-NH2
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isolated proteinase MvP1 is non-lethal to salmon at concentrations up to 0.22 microg/g fish. It causes considerable tissue necrosis and hemorrhages at the site of injection, and affects cell-cell adhesions in EPC and BF-2 cell lines, but is not highly cytotoxic. The peptidase partially degrades fish IgM heavy chain but is non-hemolytic
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additional information
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isolated proteinase MvP1 is non-lethal to salmon at concentrations up to 0.22 microg/g fish. It causes considerable tissue necrosis and hemorrhages at the site of injection, and affects cell-cell adhesions in EPC and BF-2 cell lines, but is not highly cytotoxic. The peptidase partially degrades fish IgM heavy chain but is non-hemolytic
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additional information
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treatment of Salmo salar macrophage-like cell line SHK-1 cell with peptidase MvP1 does not cause cell death. In SHK-1 cells, isolated MvP1 peptidase stimulates both interleukin 1beta and interleukin 8 expression
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additional information
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treatment of Salmo salar macrophage-like cell line SHK-1 cell with peptidase MvP1 does not cause cell death. In SHK-1 cells, isolated MvP1 peptidase stimulates both interleukin 1beta and interleukin 8 expression
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additional information
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overview: specificity towards extended substrates
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additional information
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preference for bonds of the type X-Phe, X-Leu, X-Tyr to which a hydrophobic residue contributes the amino group, and whereas it is essential that the NH2-terminal position of dipeptide substrates be blocked, the enzyme hydrolyzes substrates with free carboxyl groups
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