3.4.24.20: peptidyl-Lys metalloendopeptidase

This is an abbreviated version!
For detailed information about peptidyl-Lys metalloendopeptidase, go to the full flat file.

Reaction

Preferential cleavage in proteins: -Xaa-/-Lys- (in which Xaa may be Pro) =

Synonyms

Am-LysN, Armillaria mellea neutral proteinase, aspzincin metalloendopeptidase, aspzincin metalloprotease, EC 3.4.99.30, EC 3.4.99.32, GFMEP, Lys-N, LysN, MEP, peptidyl-Lys metalloendopeptidase, peptidyl-Lys metallopeptidase, Peptidyllysine metalloproteinase, POMEP, Proteinase, peptidyllysine metallo-

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.20 peptidyl-Lys metalloendopeptidase

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Results	in table
62	AA Sequence
4	Application
1	CAS Registry Number
1	Cloned(Commentary)
2	Crystallization (Commentary)
3	General Information
2	General Stability
60	Inhibitors
4	kcat/KM [mM/s]
1	Ki Value [mM]
15	KM Value [mM]
6	Localization
14	Metals/Ions
9	Molecular Weight [Da]
22	Organism
5	PDB ID
11	pH Optimum
4	pH Range
6	pH Stability
11	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
18	Reference
5	Source Tissue
4	Specific Activity [micromol/min/mg]
2	Storage Stability
99	Substrates and Products (Substrate)
4	Subunits
20	Synonyms
6	Temperature Optimum [°C]
14	Temperature Stability [°C]
15	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.4.24.20 - peptidyl-Lys metalloendopeptidase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3 - 11

unidentified myxobacterium

-

1 h, stable at 37°C, azocoll as substrate

31049

4 - 7

unidentified myxobacterium

-

18 h stable at 37°C, azocoll as substrate

31049

4 - 9

Pleurotus ostreatus

-

18 h, 10% loss of caseinolytic activity at 4°C

31048

5 - 10

Grifola frondosa

-

18 h, about 20% loss of activity at 4°C

31047

7.2

Grifola frondosa

-

3 h, stable at 70°C and below, 40% loss of activity at 80°C, inactivation within 30 min at 100°C

31047

9

Grifola frondosa

-

3 h, about 30%, 55% and 90% loss of activity at 50°C, 60°C and 70°C, respectively

31047