This is an abbreviated version!
For detailed information about neurolysin, go to the full flat file.

Word Map on EC


Preferential cleavage in neurotensin: Pro10-/-Tyr =


More, neurotensin endopeptidase, peptidase, neurotensin endo, endopeptidase 24.16, oligopeptidase M, peptidase, neurotensin endo-, thimet peptidase II, MOP, EP24.16c, EP24.16m, endopeptidase 24.16B, MEP, Microsomal endopeptidase, SABP, Soluble angiotensin-binding protein, ep24.16, thimet oligopeptidase II, soluble angiotensin II-binding protein, NEL, EP 24.16, neurolisin, neurolysin, Nln, mitochondrial peptidase, endopeptidase, neurotensin-cleaving enzyme


     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases


Cloned on EC - neurolysin

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CLONED (Commentary)
enzyme cloning from isolated ciliary body and liver, expression analysis
enzyme cloning from isolated ciliary body, expression analysis
exhibits 60.2% homology with EC
expressed in HEK293 cells
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression of neurolysin gene fron brain ligated to the C-terminal half of the alpha-agglutinin gene with a FLAG tag sequence in Saccharomyces cerevisiae at the cell surface, construction of a molecular displaying plasmid
expression of the FLAG-tagged brain enzyme in Saccharomyces cerevisiae strain BJ2168 on the cell surface, subcloning in Escherichia coli strain DH5alpha
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene NLN
gene NLN, sequence comparisons and phylogenetic tree, transient transfection of HeLa cells with C-terminally myc-tagged full-length human NLN (hNLN1-704) followed by immunolocalization reveals a typical mitochondrial pattern. Transfection with a construct lacking the first 25 aa (region containing the mTP as predicted by TargetP) abolishes the mitochondrial localization of hNLN
overexpressed in human kidney cells, HK293 cells. The transfectants exhibit a membrane-associated form of endopeptidase-24.16, the catalytic site of which clearly faces the extracellular domain. Transfected cells are unable to secrete the enzyme
overexpression of enzyme form EP24.16m and EP24.16c in Escherichia coli
recombinant enzyme expression in Escherichia coli
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli