3.4.24.16: neurolysin

This is an abbreviated version!
For detailed information about neurolysin, go to the full flat file.

Word Map on EC 3.4.24.16

Reaction

Preferential cleavage in neurotensin: Pro10-/-Tyr =

Synonyms

More, neurotensin endopeptidase, peptidase, neurotensin endo, endopeptidase 24.16, oligopeptidase M, peptidase, neurotensin endo-, thimet peptidase II, MOP, EP24.16c, EP24.16m, endopeptidase 24.16B, MEP, Microsomal endopeptidase, SABP, Soluble angiotensin-binding protein, ep24.16, thimet oligopeptidase II, soluble angiotensin II-binding protein, NEL, EP 24.16, neurolisin, neurolysin, Nln, mitochondrial peptidase, endopeptidase 3.4.24.16, neurotensin-cleaving enzyme

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.16 neurolysin

Cloned

Cloned on EC 3.4.24.16 - neurolysin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme cloning from isolated ciliary body and liver, expression analysis
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enzyme cloning from isolated ciliary body, expression analysis
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exhibits 60.2% homology with EC 3.4.24.15
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expressed in HEK293 cells
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expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha
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expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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expression of neurolysin gene fron brain ligated to the C-terminal half of the alpha-agglutinin gene with a FLAG tag sequence in Saccharomyces cerevisiae at the cell surface, construction of a molecular displaying plasmid
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expression of the FLAG-tagged brain enzyme in Saccharomyces cerevisiae strain BJ2168 on the cell surface, subcloning in Escherichia coli strain DH5alpha
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expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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gene NLN
gene NLN, sequence comparisons and phylogenetic tree, transient transfection of HeLa cells with C-terminally myc-tagged full-length human NLN (hNLN1-704) followed by immunolocalization reveals a typical mitochondrial pattern. Transfection with a construct lacking the first 25 aa (region containing the mTP as predicted by TargetP) abolishes the mitochondrial localization of hNLN
overexpressed in human kidney cells, HK293 cells. The transfectants exhibit a membrane-associated form of endopeptidase-24.16, the catalytic site of which clearly faces the extracellular domain. Transfected cells are unable to secrete the enzyme
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overexpression of enzyme form EP24.16m and EP24.16c in Escherichia coli
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recombinant enzyme expression in Escherichia coli
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli