3.4.24.14: procollagen N-endopeptidase
This is an abbreviated version!
For detailed information about procollagen N-endopeptidase, go to the full flat file.
Word Map on EC 3.4.24.14
Reaction
Cleaves the N-propeptide of collagen chain alpha1(I) at Pro-/-Gln and of alpha1(II) and alpha2(I) at Ala-/-Gln
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Synonyms
ADAM-TS2, ADAMTS 3, ADAMTS proteinase, ADAMTS-2, ADAMTS14, ADAMTS2, ADAMTS3, aminoprocollagen endopeptidase, aminoprocollagen peptidase, aminoterminal procollagen peptidase, PC I-NP, pNPI, procollagen ADAMTS, procollagen aminopeptidase, procollagen aminoterminal protease, Procollagen I N-proteinase, Procollagen I/II amino-propeptide processing enzyme, procollagen I/II N-endopeptidase, procollagen III N-endopeptidase, procollagen III N-proteinase, Procollagen N-endopeptidase, procollagen N-protease, procollagen N-proteinase, procollagen N-terminal peptidase, procollagen N-terminal proteinase, Procollagen peptidase, type I/II procollagen N-proteinase
ECTree
Engineering
Engineering on EC 3.4.24.14 - procollagen N-endopeptidase
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additional information
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ADAMTS2 mutated at the furin-cleavage site separating the pro domain and the metalloprotease domain lacks enzymatic activity
additional information
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removal of the PNP domain, which significantly increases enzyme activity, removal of the fourth and the second TSPI repeats, which represses the enzymatic activity, no activity when lacking the central domain or mutated at the catalytic site, mutation of the potential cleavage site by furin strongly represses, but not abolishes enzyme activity, removal of adjacent domains completely suppresses activity, use of chimeric enzyme shows significant level of activity only when the metalloprotease domain or the other central domains are present, replacement of the C-terminal domains by GON-1 or ADAMTS-14 results in reduced activity
additional information
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three constructs coding for catalytically inactive ADAMTS-2 or for ADAMTS-2 lacking either the central or the C-terminal domains are created and used for stable transfection of HEK 293-EBNA cells. Results show that the catalytic activity of ADAMTS-2 is dispensable for antiangiogenic activity
additional information
in vivo, the formation of tumors in nude mice by HEK cells is strongly reduced when they overexpress ADAMTS2, an observation that is correlated to a reduced intratumoral vascularization but that can also involve direct anti-tumor effects
additional information
in vivo, the formation of tumors in nude mice by HEK cells is strongly reduced when they overexpress ADAMTS2, an observation that is correlated to a reduced intratumoral vascularization but that can also involve direct anti-tumor effects
additional information
in vivo, the formation of tumors in nude mice by HEK cells is strongly reduced when they overexpress ADAMTS2, an observation that is correlated to a reduced intratumoral vascularization but that can also involve direct anti-tumor effects
additional information
generation of ADAMTS2-KO mice, phenotype, overview
additional information
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generation of ADAMTS2-KO mice, phenotype, overview