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3.4.23.49: omptin

This is an abbreviated version!
For detailed information about omptin, go to the full flat file.

Word Map on EC 3.4.23.49

Reaction

Has a virtual requirement for Arg in the P1 position and a slightly less stringent preference for this residue in the P1' position, which can also contain Lys, Gly or Val. =

Synonyms

bacterial outer-membrane protease, Citrobacter rodentium outer-membrane protease, CroP, E. coli protease VII, EC 3.4.21.87, endoprotease, Gene ompT proteins, More, OmpP, OmpP protease, ompT, OmpT protease, OmpT protein, Omptin, omptin protease, outer membrane protease, Outer membrane protein 3B, outer-membrane protease, outer-membrane protease T, PgtE, Pla, plaA, protease 7, Protease A, Protease VII, Protein a, Proteins, specific or class, gene ompT, SopA

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.49 omptin

Cloned

Cloned on EC 3.4.23.49 - omptin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21, mutation in OmpT protein characterized in BL21 producing fusion protein GST-Sup35NM
expressed in Escherichia coli XL1-Blue, additional strains and plasmids listed, construction of phage libraries and substrate phage described
expression in Escherichia coli
expression of wild-type and mutant enzymes
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gene croP, recombinant expression of wild-type enzyme in DELTAcroP-deletion mutant strain of Citrobacter rodentium
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gene ompT is located on the chromosome and a cryptic prophage, gene ompP is 95 kb F-plasmid-encoded, phylogenetic tree of the omptin family
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gene ompT, phage display metod used for substrate specificity analysis, expression in Escherichia coli AN1 cells
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gene pgtE, DNA and amino acid sequence determination and analysis
gene pla is 9.5 kb plasmid pPCP1 encoded, phylogenetic tree of the omptin family
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inserted into pET-32a, expressed in Escherichia coli
inserted into pTrc99A, expressed in Escherichia coli, additional strains and plasmids of the study listed
overexpressed without its signal sequence in Escherichia coli K-12 strain DH5alpha using a T7 system
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overexpression of wild-type and mutant enzymes strain W3110 M25
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recombinant expression of OmpT in inclusion bodies
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recombinant expression of the chimeric C-terminally His-tagged and mCherry fluorescence-tagged surface anchor Lpp-OmpT (LOT) in Escherichia coli strain BL21(DE3)
recombinant expression of wild-type pgtE in Escherichia coli strain 83972 DELTAompT, which is deleted for the endogenous omptin gene ompT and lacks ompP. Escherichia coli strain 83972 DELTAompT expressing PgtE cleaves B in a time-dependent fashion generating four immunoreactive fragments
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the gene pgtE is encoded on the chromosome, phylogenetic tree of the omptin family
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the gene plaA is 36 kb plasmid pEP36 encoded, phylogenetic tree of the omptin family
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the gene sopA is 210 kb virulence plasmid pWR100-encoded, phylogenetic tree of the omptin family
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