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3.4.23.4: chymosin

This is an abbreviated version!
For detailed information about chymosin, go to the full flat file.

Word Map on EC 3.4.23.4

Reaction

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single Ser-Phe105-/-Met-Ala bond in kappa-chain of casein =

Synonyms

CHY-MAX, CHY-MAX 200, chymase, chymosin, chymosin A, chymosin B, CYM, EC 3.4.4.3, More, Preprorennin, prochymosin, rCH, rennin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.4 chymosin

Purification

Purification on EC 3.4.23.4 - chymosin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Q Sepharose column chromatography, and Sephadex S-100 gel filtration
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by affinity chromatography MIMO1300 matrix
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by affinity chromatography on a bacitracin-Sepharose column
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DEAE-cellulose column chromatography, gel filtration
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extraction by application of ultrasound
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gel filtration and anion-exchange chromatography
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metal affinity chromatography
Ni-NTA affinity column chromatography
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phenyl Superose column chromatography
purification by partitioning between aqueous two-phase systems
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resolves into three major peaks in DEAE chromatography
similar enzyme
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ultrafiltration and Superdex 75 gel filtration
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