3.4.23.4: chymosin
This is an abbreviated version!
For detailed information about chymosin, go to the full flat file.
Word Map on EC 3.4.23.4
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3.4.23.4
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milk
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casein
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cheese
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pepsin
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calf
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coagulation
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kappa-caseins
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rennet
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milk-clotting
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clot
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proteinases
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angiotensin
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hypertension
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aldosterone
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beta-caseins
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micelle
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whey
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pepsinogen
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renin
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ripening
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plasmin
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zymogen
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curd
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camel
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skim
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mucor
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cheddar
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pepstatin
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abomasum
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gelation
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cardunculus
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preruminant
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cynara
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progastricsins
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miehei
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fundic
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beta-cn
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food industry
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pusillus
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gastricsins
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urea-page
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s1-casein
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beta-lactoglobulin
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parasitica
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hypokalemia
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glycomacropeptide
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cheese-making
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rhizomucor
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3.4.23.1
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synthesis
- 3.4.23.4
- milk
- casein
-
cheese
- pepsin
-
calf
-
coagulation
- kappa-caseins
- rennet
-
milk-clotting
- clot
- proteinases
- angiotensin
- hypertension
- aldosterone
- beta-caseins
-
micelle
- whey
- pepsinogen
- renin
-
ripening
- plasmin
- zymogen
-
curd
- camel
-
skim
- mucor
-
cheddar
- pepstatin
- abomasum
-
gelation
- cardunculus
-
preruminant
-
cynara
- progastricsins
- miehei
-
fundic
-
beta-cn
- food industry
- pusillus
- gastricsins
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urea-page
-
s1-casein
- beta-lactoglobulin
- parasitica
- hypokalemia
- glycomacropeptide
-
cheese-making
- rhizomucor
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3.4.23.1
- synthesis
Reaction
Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single Ser-Phe105-/-Met-Ala bond in kappa-chain of casein =
Synonyms
CHY-MAX, CHY-MAX 200, chymase, chymosin, chymosin A, chymosin B, CYM, EC 3.4.4.3, More, Preprorennin, prochymosin, rCH, rennin
ECTree
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KM Value
KM Value on EC 3.4.23.4 - chymosin
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0.003
Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine
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hydrolysis by calf chymosin
0.165
undecapeptide analogue to chymosin sensitive region of bovine kappa casein
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-
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0.077
undecapeptide analogue to chymosin sensitive region of bovine kappa-casein
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-
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0.134
undecapeptide analogue to chymosin sensitive region of camel kappa casein
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-
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0.056
undecapeptide analogue to chymosin sensitive region of camel kappa-casein
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-
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0.0056
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hydrolysis by recombinant chymosin
0.0056
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine
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hydrolysis by transgenic chymosin
0.007
Abz-A-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine
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hydrolysis by calf chymosin
0.0022
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hydrolysis by calf chymosin
0.0035
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine
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hydrolysis by recombinant chymosin
0.018
Abz-A-A-F-F-A-N-(2,4-dinitrophenyl)-ethylenediamine
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hydrolysis by transgenic chymosin
0.0018
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hydrolysis by calf chymosin
0.0021
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine
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hydrolysis by recombinant chymosin
0.0078
Abz-A-F-F-A-A-N-(2,4-dinitrophenyl)-ethylenediamine
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hydrolysis by transgenic chymosin
0.016
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hydrolysis of the Phe148 variant (cleavage L147-F148) by D289M/Q298L mutant chymosin
0.024
NT/NMN 142-151
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hydrolysis of the Phe148 variant (cleavage F147-R148) by D289M/Q298L mutant chymosin
0.028
NT/NMN 142-151
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hydrolysis of the Phe148 variant (cleavage F147-R148) by Asp289Met mutant chymosin
0.029
NT/NMN 142-151
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hydrolysis of the Phe148 variant (cleavage L147-F148) by Gln298Leu mutant chymosin
0.033
NT/NMN 142-151
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hydrolysis of the Phe148 variant (cleavage F147-R148) by Gln298Leu mutant chymosin
0.046
NT/NMN 142-151
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hydrolysis of the Glu148 variant by D289M/Q298L mutant chymosin
0.054
NT/NMN 142-151
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hydrolysis of the Glu148 variant by Asp289Met mutant chymosin
0.054
NT/NMN 142-151
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hydrolysis of the Phe148 variant (cleavage L147-F148) by Asp289Met mutant chymosin
0.059
NT/NMN 142-151
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hydrolysis of the parent peptide by wild type chymosin
0.062
NT/NMN 142-151
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hydrolysis of the Glu148 variant by Gln298Leu mutant chymosin
0.072
NT/NMN 142-151
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hydrolysis of the parent peptide by Asp289Met mutant chymosin
0.083
NT/NMN 142-151
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hydrolysis of the Phe148 variant (cleavage L147-F148) by wild type chymosin
0.087
NT/NMN 142-151
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hydrolysis of the parent peptide by D289M/Q298L mutant chymosin
0.095
NT/NMN 142-151
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hydrolysis of the parent peptide by Gln298Leu mutant chymosin
0.105
NT/NMN 142-151
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hydrolysis of the Phe148 variant (cleavage F147-R148) by wild type chymosin
0.111
NT/NMN 142-151
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hydrolysis of the Glu148 variant by wild type chymosin
0.065
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hydrolysis of the Glu171 variant by Asp289Met mutant chymosin
0.114
POm C165-174
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hydrolysis of the Glu171 variant by D289M/Q298L mutant chymosin
0.229
POm C165-174
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hydrolysis of the parent peptide by Gln298Leu mutant chymosin
0.25
POm C165-174
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hydrolysis of the parent peptide by D289M/Q298L mutant chymosin
0.288
POm C165-174
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hydrolysis of the parent peptide by Asp289Met mutant chymosin
0.304
POm C165-174
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hydrolysis of the Glu171 variant by wild type chymosin
0.375
POm C165-174
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hydrolysis of the parent peptide by wild type chymosin
0.463
POm C165-174
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hydrolysis of the Glu171 variant by Gln298Leu mutant chymosin
additional information
additional information
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Km of kappa-casein related peptides
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additional information
additional information
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transgenic and recombinant chymosin do not hydrolyze substrate Abz-A-A-F-F-A-A-p-nitroanilide, Abz-A-A-F-F-N-(2,4-dinitrophenyl)-ethylenediamine and Abz-A-A-F-F-p-nitroanilide
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