Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.23.4: chymosin

This is an abbreviated version!
For detailed information about chymosin, go to the full flat file.

Word Map on EC 3.4.23.4

Reaction

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single Ser-Phe105-/-Met-Ala bond in kappa-chain of casein =

Synonyms

CHY-MAX, CHY-MAX 200, chymase, chymosin, chymosin A, chymosin B, CYM, EC 3.4.4.3, More, Preprorennin, prochymosin, rCH, rennin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.4 chymosin

Cloned

Cloned on EC 3.4.23.4 - chymosin

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as inclusion bodies in Escherichia coli
-
expressed in Escherichia coli and in Saccharomyces cerevisiae
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Komagataella phaffii (Pichia pastoris strain GS115)
expressed in Nicotiana tabacum cultivar Petite Havana
-
expressed in Pichia pastoris strain GS115
expressed in Pichia pastoris strain KM71H
-
expressed in Pichia pastoris strain X-33
expressed in Zea mays seeds
expression in Aspergillus niger
-
expression in Aspergillus niger var. awamori dgr246pyrG
-
expression in Kluyveromyces lactis
-
expression in Pichia pastoris
-
expression in Saccharomyces cerevisiae strain INVSC1
-
loop exchange mutant expressed in Trichoderma reesei
-
point mutants expressed in Escherichia coli and Trichoderma reesei
-
prochymosin cDNA, expressed as a NusA fusin protein in Escherichia coli, low level of milk clotting activity after activation at acidic pH
-
prochymosin fusion product expressed in Escherichia coli
-
S351T mutant of prochymosin as fusion protein with the Aspergillus niger enzyme glucoamylase in Aspergillus niger var. awamori, insertion of a short peptide linker between the proteins with the sequence TDNST
-
the enzyme as a fusion with Tol-A-III protein is expressed in Escherichia coli BL21 (DE3) cells
-