3.4.23.25: saccharopepsin
This is an abbreviated version!
For detailed information about saccharopepsin, go to the full flat file.
Reaction
Hydrolysis of proteins with broad specificity for peptide bonds. Cleaves -Leu-Leu-/-Val-Tyr bond in a synthetic substrate. Does not act on esters of Tyr or Arg
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Synonyms
Acid protease, Aspartate protease, Aspartic proteinase, Aspartic proteinase yscA, EC 3.4.23.6, EC 3.4.23.8, EC 3.4.4.17, Pep4, PEP4 gene product, Pep4p, Pep4p vacuolar proteinase, pepsin-like aspartic proteinase, PRA, preproPrA, proPrA, Protease A, Proteinase A, proteinase A precursor, Proteinase yscA, Proteinase, yeast A, proteinase-A, pseudo-proteinase A, Saccharomyces aspartic proteinase, Saccharomyces cerevisiae aspartic proteinase A, saccharopepsin, vacuolar aspartic proteinase, yeast aspartic proteinase A, Yeast endopeptidase A, yeast proteinase, Yeast proteinase A
ECTree
Inhibitors
Inhibitors on EC 3.4.23.25 - saccharopepsin
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1,2-epoxy-3-(4-nitrophenoxy) propane
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-
1,2-epoxy-3-(4-nitrophenoxy)propane
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-
1,2-epoxy-3-(p-nitrophenoxy) propane
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Diazoacetyl-D,L-norleucine methyl ester
-
Diazoacetyl-DL-norleucine methyl ester
HgCl2
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complete inhibition at 0.05 mM
iodoacetate
-
weak inhibitor
leupeptin
-
strong inhibitor
natural aspartic proteinase inhibitor IA3
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-
-
natural aspartic proteinase inhibitor IA4
-
-
-
p-chloromercuribenzoic acid
-
complete inhibition at 0.1 mM
potato aspartic proteinase inhibitor
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-
-
protein IA3
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Saccharomyces cerevisiae protein
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protein IA3 mutant 2-34
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-
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protein IA3 mutant M31-M32
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-
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Specific proteinase inhibitor from yeast
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tomato aspartic proteinase inhibitor
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-
-
additional information
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insensitive to diisopropylphosphofluoridate, metal chelating agents and thiol poisons
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CP-108,420
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-
CP-72,647
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-
CP-81,198
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-
CP-81,282
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-
Diazoacetyl-DL-norleucine methyl ester
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in presence of copper ions
Diazoacetyl-DL-norleucine methyl ester
-
-
IA3
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endogenous 68-amino acid inhibitor. In absence of enzyme, IA3 is unstructured, addition of enzyme leads to large spectral transition of IA3
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IA3
peptide inhibitor synthesized by Saccharomyces cerevisae, a synthetic peptide containing residues 2-34 of IA3 exhibits comparable potency to natural IA3
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IA3
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highly specific polypeptide inhibitor synthesized by Saccharomyces cerevisiae, a hydrogen bond network also residues K18 and D22 of the inhibitor directly to the catalytic Asp-32 and Tyr-75 residues of the enzyme, thus deadlocking the inhibitor in position, numerous mutants of IA3 created and tested for inhibition, all mutants are found to be inhibitory although to different extent, variations within residues 2-32 at positions other than 18 and 22 appears to have relatively minor effects
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IA3
68 residue peptide, KI below 1 nM
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PD-129,541
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cyclic peptide inhibitor
PD-133,450
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-
Specific proteinase inhibitor from yeast
-
-
-
Specific proteinase inhibitor from yeast
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completely selective for proteinase A from yeast; Saccharomyces cerevisiae
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