3.4.23.21: Rhizopuspepsin
This is an abbreviated version!
For detailed information about Rhizopuspepsin, go to the full flat file.
Reaction
hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1'. Clots milk and activates trypsinogen. Does not cleave Gln4-His, but does cleave His10-/-Leu and Val12-/-Glu in B chain of insulin =
Synonyms
Aspartate protease, aspartic peptidase, EC 3.4.23.6, EC 3.4.23.9, EC 3.4.4.17, EC 3.4.99.25, More, Neurase, Proteinase, Rhizopus acid, Rhizopus acid protease, Rhizopus acid proteinase, Rhizopus aspartic proteinase, rhizopuspepsinogen
ECTree
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Purification
Purification on EC 3.4.23.21 - Rhizopuspepsin
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2 major isoenzymes with pI 5.1 and 5.8, 3 minor isoenzymes with pI 7.35, 7.41 and 7.9, partial
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native enzyme 91fold by ammonium sulfate fractionation, ion exchange chromatography and gel filtration
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native enzyme from culture medium
native enzyme from culture medium by ammonium sulfate precipitation and pepstatin affinity chromatography, further separation of isozymes by isoelectric focusing, recombinant enzyme from Escherichia coli inclusion bodies
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rapid, efficient 1-step purification, high-performance hydrophobic interaction chromatography of proteins on reversed-phase supports coated with non-ionic surfactants of polyoxyethylene type
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