3.4.23.19: Aspergillopepsin II
This is an abbreviated version!
For detailed information about Aspergillopepsin II, go to the full flat file.
Reaction
preferential cleavage in B chain of insulin: Asn3-/-Gln, Gly13-/-Ala, Tyr26-/-Thr =
Synonyms
acid proteinase, acid proteinase A, AGP, aspergilloglutamic peptidase, Aspergillus niger var. macrosporus aspartic proteinase, Aspergillus proteinase A, EC 3.4.23.6, More, non-pepsin type acid proteinase, Proctase A, Proteinase A
ECTree
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Renatured
Renatured on EC 3.4.23.19 - Aspergillopepsin II
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recombinant wild-type and mutant enzymes, denaturation by 8 M urea, refolding after dialysis in 50 mM sodium acetate, pH 5.3, 10 mM 2-mercaptoethanol, at 4°C overnight
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