3.4.22.40: bleomycin hydrolase
This is an abbreviated version!
For detailed information about bleomycin hydrolase, go to the full flat file.
Word Map on EC 3.4.22.40
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3.4.22.40
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luteinizing
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paraoxonase
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thiolactone
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helveticus
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peplomycin
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lutropins
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corneocytes
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homocysteinylation
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beta-naphthylamide
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medicine
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hcy-thiolactone
- 3.4.22.40
-
luteinizing
- paraoxonase
- thiolactone
- helveticus
- peplomycin
-
lutropins
- corneocytes
-
homocysteinylation
- beta-naphthylamide
- medicine
-
hcy-thiolactone
Reaction
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred =
Synonyms
aminopeptidase C, aminopeptidase H, ApsC, BANA-hydrolase, BH, BH protein, Bleomycin hydrolase, BLH, Blh1p, BLM hydrolase, Blmh, BLMH protein, BMH, citrulline aminopeptidase, Gal6p, Hcy-thiolactonase, homocysteine thiolactonase, HTLase, hydrolase H, More, PEPC, yBLH, yeast cysteine protease
ECTree
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Purification
Purification on EC 3.4.22.40 - bleomycin hydrolase
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native enzyme 4300fold from newborn rat epidermis by gel filtration, and two steps of anion exchange chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
native enzyme 4300fold from newborn rat epidermis by gel filtration, and two steps of anion exchange chromatography
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native enzyme 4300fold from newborn rat epidermis by gel filtration, and two steps of anion exchange chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
-