3.4.22.40: bleomycin hydrolase

This is an abbreviated version!
For detailed information about bleomycin hydrolase, go to the full flat file.

Word Map on EC 3.4.22.40

Reaction

Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred =

Synonyms

More, PEPC, aminopeptidase C, hydrolase H, BANA-hydrolase, Blh1p, Gal6p, aminopeptidase H, Bleomycin hydrolase, BLM hydrolase, BMH, ApsC, BH, yeast cysteine protease, yBLH, homocysteine thiolactonase, Hcy-thiolactonase, HTLase, BLH, BH protein, BLMH protein, citrulline aminopeptidase, Blmh

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.40 bleomycin hydrolase

KM Value

KM Value on EC 3.4.22.40 - bleomycin hydrolase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.805
6'-deoxy bleomycin A2
pH 7.5, 30°C
0.942
6'-deoxy bleomycin Z
pH 7.5, 30°C
0.72
6'-deoxy tallysomycin H1
pH 7.5, 30°C
17.4
Ala-p-nitroanilide
-
-
6.1
Arg-p-nitroanilide
-
-
28.4
Asp-p-nitroanilide
-
-
0.46 - 1.3
bleomycin A2
0.056 - 0.79
bleomycin B2
0.854
bleomycin Z
pH 7.5, 30°C
2.25
butylamino-3-propylamino-3-propylamine bleomycin
-
-
0.49
deglyco bleomycin A2
-
-
2.9
Gly-Ala
-
-
14.8
Gly-Asp
-
-
5.6
Gly-D-Leu
-
-
10.1
Gly-Gly
-
-
0.66
Gly-Gly-Ala
-
-
0.75
Gly-Gly-Arg
-
-
1.01
Gly-Gly-D-Leu
-
-
1.14
Gly-Gly-Glu
-
-
1.05
Gly-Gly-Gly
-
-
0.13
Gly-Gly-Gly-Ala
-
-
0.78 - 1.24
Gly-Gly-Gly-Gly
0.57
Gly-Gly-Leu
-
-
4.1
Gly-Gly-NH2
-
-
0.29
Gly-Gly-Phe
-
-
0.2
Gly-Gly-Phe-Leu
-
-
1.05
Gly-Gly-Tyr
-
-
4.6
Gly-Leu
-
-
4.7
Gly-Lys
-
-
13.5
Gly-NH2
-
-
9.7
Gly-p-nitroanilide
-
-
1.7
Gly-Phe
-
-
12.5
Leu-p-nitroanilide
-
-
9.9
Lys-p-nitroanilide
-
-
9.5
Met-Ala
-
-
0.5
Met-Ala-Ser
-
-
7
Met-paranitroanilide
-
-
0.7 - 0.79
peplomycin
3.5
Phe-p-nitroanilide
-
-
0.743
tallysomycin H1
pH 7.5, 30°C
0.45
tallysomycin S10b
-
-
2.7
Thr-Ser
-
-
0.63
Thr-Ser-Lys
-
-