3.4.22.31: Ananain
This is an abbreviated version!
For detailed information about Ananain, go to the full flat file.
Word Map on EC 3.4.22.31
Reaction
hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain =
Synonyms
bromelain protease F9
ECTree
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General Information on EC 3.4.22.31 - Ananain
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GENERAL INFORMATION 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
evolution
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despite a high degree of sequence identity between ananain and stem bromelain, the most abundant bromelain cysteine protease, ananain displays distinct chemical properties, substrate preference and inhibitory profile compared to stem bromelain. Ananain belongs to the papain family of peptidases with a high degree of identity to chymopapain (52%) and papain (60%) from papaya
physiological function
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ananain accounts for less than 10% of the total enzyme in the crude pineapple stem extract known as bromelain, yet yields the majority of the proteolytic activity of bromelain
additional information
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the enzyme shows a geometrically flat and open S1 subsite for ananain. This subsite accommodates diverse P1 substrate residues, while a narrow and deep hydrophobic pocket-like S2 subsite would accommodate a non-polar P2 residue, such as the preferred Leu residue observed in specificity studies
