3.4.22.31: Ananain

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For detailed information about Ananain, go to the full flat file.

Reaction

hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain =

Synonyms

bromelain protease F9

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.31 Ananain

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Results	in table
2	Activating Compound
8	AA Sequence
1	CAS Registry Number
1	Cloned(Commentary)
1	Crystallization (Commentary)
3	General Information
9	Inhibitors
11	kcat/KM [mM/s]
22	KM Value [mM]
4	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
9	Organism
12	PDB ID
3	pH Optimum
1	pH Range
1	Posttranslational Modification
2	Protein Variants
6	Purification (Commentary)
3	Reaction
3	Reaction Type
9	Reference
1	Renatured (Commentary)
8	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
42	Substrates and Products (Substrate)
6	Subunits
1	Synonyms
2	Temperature Optimum [°C]
21	Turnover Number [1/s]

Engineering

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Engineering on EC 3.4.22.31 - Ananain

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E35A

Ananas comosus

P80884

site-directed mutagenesis, exchange of the conserved residue functional as part of the so-called electrostatic switch, mutation leads to an increased instability of the refolded recombinant pro-enzyme, different activation conditions are required, altered pH profile

649918

E50A

Ananas comosus

P80884

site-directed mutagenesis, exchange of the conserved residue functional as part of the so-called electrostatic switch, slightly altered pH profile and slightly enhanced activity, activation similar to the wild-type

649918