3.4.22.31: Ananain
This is an abbreviated version!
For detailed information about Ananain, go to the full flat file.
Word Map on EC 3.4.22.31
Reaction
hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-/-NHMec, but broader specificity than fruit bromelain =
Synonyms
bromelain protease F9
ECTree
3.4.22.31 AnanainAdvanced search results
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Crystallization on EC 3.4.22.31 - Ananain
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CRYSTALLIZATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
purified native enzyme free and in complex with inhibitor E-64, hanging drop vapor diffusion technique, mixing of 10 mg/ml protein in 20 mM Tris, pH 8.0, and 20 mM NaCl with reservoir solution containing 72% w/v MPD, and 0.1 M Tris, pH 8.8, 20°C, 2 weeks, the enzyme-inhibitor complex is built from activated ananain mixed with inhibition buffer containing 20 mM sodium acetate, pH 5.5, 300 mM KCl, 1 mM EDTA, 10 mM L-cysteine, 5% v/v DMSO, with 0.5 mM E-64 at a final concentration of 0.1 mM and incubation at room temperature for 3 h. The inhibited ananain is desalted and equilibrated with 20 mM Tris, pH 8.0, and 20 mM NaCl and then concentrated to 6.5 mg/ml, crystals of E-64-ananain are obtained in the same condition as for unbound ananain, X-ray diffraction structure determination and analysis at 1.73 and 1.98 A resolution, respectively
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