3.4.22.10: streptopain

This is an abbreviated version!
For detailed information about streptopain, go to the full flat file.

Word Map on EC 3.4.22.10

Reaction

preferential cleavage with hydrophobic residues at P2, P1 and P1' =

Synonyms

More, Streptococcus peptidase A, Streptococcus protease, EC 3.4.4.18, Streptococcal cysteine proteinase, Steptococcus proteinase, proteinase, streptococcal, SCP, Spe B, streptococcal erythrogenic toxin B, streptococcal pyrogenic exotoxin B, SPP, SpeB, streptococcal proteinase, interleukin-1beta convertase, pyrogenic exotoxin B, streptococcal pyrogenic exotoxin B/cysteine protease, SPE B/SCP, SPE B protease, streptococcal cysteine protease, IdeS, IgG-degrading enzyme of Streptococcus pyogenes, SpcCEP, streptococcal pyogenic exotoxin B, streptopain

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.10 streptopain

Substrates Products

Substrates Products on EC 3.4.22.10 - streptopain

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-aminobenzoyl-Ala-Ala-Ile-Lys-Ala-Gly-Ala-Arg + H2O
?
show the reaction diagram
3-aminobenzoyl-Ala-Glu-Ile-Lys-Gln-Pro-Val-Val + H2O
?
show the reaction diagram
-
-
-
-
?
3-aminobenzoyl-Asp-Lys-Val-Asn-Leu-Gly-Gly-Glu + H2O
?
show the reaction diagram
3-aminobenzoyl-Glu-Gln-Ile-Lys-Glu-Asn-Lys-Lys + H2O
?
show the reaction diagram
3-aminobenzoyl-Thr-Thr-Thr-Ala-Gly-Thr-Ala-Glu + H2O
?
show the reaction diagram
acetyl-Ala-Ile-Arg-7-amino-4-methylcoumarin + H2O
?
show the reaction diagram
alpha1-antitrypsin + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Ala + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Glu-p-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Glu-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Gly-p-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
benzyloxycarbonyl-Gly + p-nitrophenol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Nle-p-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Nle-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Val-Arg-4-methylcoumarinyl-7-amide + H2O
?
show the reaction diagram
Bovine serum albumin + H2O
?
show the reaction diagram
C5a peptidase + H2O
?
show the reaction diagram
-
cleavage sites are APA-K, AVI-D, SGTS, and C-terminally
-
-
?
casein + H2O
?
show the reaction diagram
cinnamoyl-Gly-p-nitrophenyl ester + H2O
cinnamoyl-Gly + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
complement component C3 + H2O
?
show the reaction diagram
-
-
-
-
?
complement factor C3b + H2O
?
show the reaction diagram
-
cleavage sites are RTL-D, NHK-L, LAR-S, and VEL-I
-
-
?
D-Ile-Pro-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
decorin + H2O
?
show the reaction diagram
-
-
-
-
?
E-cadherin + H2O
?
show the reaction diagram
-
-
-
-
?
EndoS + H2O
?
show the reaction diagram
-
cleavage site is VML-K
-
-
?
FAAIKAGARY + H2O
FAAIK + AGARY
show the reaction diagram
-
-
-
?
Fba + H2O
?
show the reaction diagram
Fba protein + H2O
?
show the reaction diagram
-
-
-
-
?
Fibrinogen + H2O
?
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
H-kininogen + H2O
?
show the reaction diagram
-
cleavage sites are LMK-R and PFR-S
-
-
?
H-kininogen + H2O
biologically active kinins
show the reaction diagram
-
-
-
?
human CXCL-1 + H2O
?
show the reaction diagram
human CXCL-2 + H2O
?
show the reaction diagram
human CXCL-6 + H2O
?
show the reaction diagram
human CXCL-8 + H2O
?
show the reaction diagram
human CXCL10 + H2O
?
show the reaction diagram
-
-
-
-
?
Human fibrinogen + H2O
?
show the reaction diagram
human interleukin 1beta precursor + H2O
human interleukin 1beta + human interleukin 1beta signal peptide
show the reaction diagram
-
-
-
-
?
IgA + H2O
?
show the reaction diagram
IgD + H2O
?
show the reaction diagram
-
degradation of the carboxy-terminal part of the heavy chain
-
?
IgE + H2O
?
show the reaction diagram
-
degradation of the carboxy-terminal part of the heavy chain
-
?
IgG + H2O
?
show the reaction diagram
IgG + H2O
Fab fragment + Gc fragments
show the reaction diagram
-
the enzyme cleaves IgG in the flexible hinge region of the IgG heavy chain, generating two Fab fragments and Fc fragment. SpeB cleaves the heavy chain at a defined site between glycine residues 236 and 237
-
?
IgM + H2O
?
show the reaction diagram
immunoglobulin A + H2O
?
show the reaction diagram
-
cleavage sites are the heavy chains
-
-
?
immunoglobulin D + H2O
?
show the reaction diagram
-
cleavage sites are the heavy chains
-
-
?
immunoglobulin E + H2O
?
show the reaction diagram
-
cleavage sites are the heavy chains
-
-
?
immunoglobulin G + H2O
?
show the reaction diagram
-
cleavage site is LLG-G in gamma-chain
-
-
?
immunoglobulin G + H2O
F(ab')2 + 1/2Fc
show the reaction diagram
-
non-immune binding of IgG to the bacterial surface is followed by the proteolytic cleavage of the antibody by the IgG-endopeptidase IdeS. IdeS generated 1/2Fc fragments do not compete efficiently with intact IgG in binding to the bacterial surface and rapid dissociation of 1/2Fc allows binding of new IgG. A correlated binding and proteolytic cleavage of IgG increases the probability that the bacteria can resist specific IgG, despite the presence of a large excess of non-specific IgG in the circulation. As a consequence of IdeS activity, circulating 1/2Fc fragments are generated. These 1/2Fc fragments are shown to be biological active by acting as priming agents for polymorphonuclear leucocytes
-
-
?
immunoglobulin G + H2O
F(ab')2 fragment + 1/2 Fc fragments
show the reaction diagram
-
IdeS cleaves IgG by hydrolyzing the peptide bond between two glycine residues in the hinge region of IgG, generating one F(ab')2 fragment and two 1/2 Fc fragments
-
-
?
immunoglobulin M + H2O
?
show the reaction diagram
-
cleavage sites are the heavy chains
-
-
?
insulin + H2O
?
show the reaction diagram
-
reduced carboxymethylated phenylalanine chain, one of the most rapidly hydrolyzed Phe-Tyr linkages is in the Phe-Phe-Tyr sequence
-
-
?
insulin beta chain + H2O
?
show the reaction diagram
-
reduced and carboxylated substrate, cleavage of the linkage Phe25-Tyr26
-
-
?
interleukin-1beta precursor + H2O
interleukin-1beta + interleukin-1beta propeptide
show the reaction diagram
Laminin + H2O
?
show the reaction diagram
-
-
-
-
?
M protein + H2O
?
show the reaction diagram
-
-
-
-
?
mouse CXCL-1 + H2O
?
show the reaction diagram
mouse CXCL-2 + H2O
?
show the reaction diagram
-
the cleavage site is between lysine residues K65 and K66
-
-
?
N-benzoyl-Gly-p-nitrophenyl ester + H2O
N-benzoyl-Gly + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Ala-p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-Ala + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Gly + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-Gly + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-Lys-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-Lys-phenyl ester + H2O
Nalpha-benzyloxycarbonyl-Lys + phenol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys-p-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-Phe-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-Phe-Tyr + H2O
?
show the reaction diagram
-
-
-
-
?
Nepsilon-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O
Nepsilon-benzyloxycarbonyl-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
occludin + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrobenzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
p-nitrobenzyloxycarbonyl-Gly + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-benzyloxycarbonyl-L-Ala + H2O
?
show the reaction diagram
-
-
-
-
?
plasminogen + H2O
?
show the reaction diagram
-
-
-
-
?
pro-interleukin-1beta + H2O
interleukin-1beta + ?
show the reaction diagram
-
cleavage site is YVH-D
-
-
?
properdin + H2O
?
show the reaction diagram
proSPE B C47S + H2O
?
show the reaction diagram
-
the proSPE B C47S mutant is also be used as the substrate for activity assay because it does not exhibit any enzyme activity and exists as a 42 kDa zymogen
-
-
?
proSpeB + H2O
SpeB + ?
show the reaction diagram
protein F1 + H2O
?
show the reaction diagram
protein H + H2O
?
show the reaction diagram
-
-
-
-
?
resorufin-labeled casein + H2O
?
show the reaction diagram
-
-
-
-
?
Sda1 + H2O
?
show the reaction diagram
-
-
-
-
?
Slr + H2O
?
show the reaction diagram
-
-
-
-
?
SmeZ + H2O
?
show the reaction diagram
-
-
-
-
?
SpeBz + H2O
mature SpeB + ?
show the reaction diagram
-
auto-cleavage sites are AIK-A, KVN-L, QIK-E, TYA-G, and EIK-Q
-
-
?
streptococcal mitogenic exotoxin SmeZ + H2O
?
show the reaction diagram
-
cleaves between glutamate and glycine residues
-
-
?
streptococcal mitogenic exotoxin SpeG + H2O
?
show the reaction diagram
-
partial degradation, SpeB cleaves between glutamate and glycine residues
-
-
?
streptococcal mitogenic exotoxin Z + H2O
?
show the reaction diagram
-
-
-
-
?
streptokinase + H2O
?
show the reaction diagram
-
-
-
-
?
streptolysin O + H2O
?
show the reaction diagram
-
-
-
-
?
t-butyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
t-butyloxycarbonyl-Gly + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Ala-p-nitrophenyl ester + H2O
tert-butyloxycarbonyl-Ala + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
urokinase receptor + H2O
?
show the reaction diagram
-
cleavage site is close to the GPI anchor
-
-
?
Vitronectin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-