3.4.22.10: streptopain

This is an abbreviated version!
For detailed information about streptopain, go to the full flat file.

Word Map on EC 3.4.22.10

3.4.22.10

speb

streptococci

glomerulonephritis

zymogen

fasciitis

superantigens

streptolysin

nephritogenic

streptokinase

poststreptococcal

scarlet

apsgn

shock-like

Reaction

preferential cleavage with hydrophobic residues at P2, P1 and P1' =

Synonyms

EC 3.4.4.18, IdeS, IgG-degrading enzyme of Streptococcus pyogenes, interleukin-1beta convertase, More, proteinase, streptococcal, pyrogenic exotoxin B, SCP, SpcCEP, Spe B, SPE B protease, SPE B/SCP, SpeB, SPP, Steptococcus proteinase, streptococcal cysteine protease, Streptococcal cysteine proteinase, streptococcal erythrogenic toxin B, streptococcal proteinase, streptococcal pyogenic exotoxin B, streptococcal pyrogenic exotoxin B, streptococcal pyrogenic exotoxin B/cysteine protease, Streptococcus peptidase A, Streptococcus protease, streptopain

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.22 Cysteine endopeptidases

3.4.22.10 streptopain

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Results	in table
6	Activating Compound
168	AA Sequence
1	CAS Registry Number
16	Cloned(Commentary)
5	Crystallization (Commentary)
2	Expression
12	General Information
5	General Stability
1	IC50 Value
60	Inhibitors
10	kcat/KM [mM/s]
1	Ki Value [mM]
34	KM Value [mM]
7	Localization
2	Metals/Ions
28	Molecular Weight [Da]
68	Natural Substrates/ Products (Substrates)
70	Organism
11	PDB ID
7	pH Optimum
1	pH Stability
15	Posttranslational Modification
37	Protein Variants
15	Purification (Commentary)
3	Reaction
1	Reaction Type
66	Reference
2	Renatured (Commentary)
2	Specific Activity [micromol/min/mg]
2	Storage Stability
165	Substrates and Products (Substrate)
13	Subunits
85	Synonyms
10	Temperature Optimum [°C]
4	Temperature Stability [°C]
32	Turnover Number [1/s]

Purification

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Purification on EC 3.4.22.10 - streptopain

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2 entries

a combination of two successive strong cation exchange resins gives the best results for soluble, pure enzyme with the highest activity

Streptococcus pyogenes

P0C0J0

755296

HiTrap SP column chromatography, and G75 Sephadex gel filtration

Streptococcus pyogenes

717218

native mature Spe B by ammonium sulfate fractionation, dialysis, and ion exchange chromatography, recombinant His-tagged Spe B propeptide and SpeB mutant C47S from Escherichia coli

Streptococcus pyogenes

670312

Ni2+-chelating column chromatography

2 entries

recombinant His-tagged wild-type and mutant C192S enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the wild-type enzyme autoprocesses during purification to the mature 28 kDa protein

Streptococcus pyogenes

667426

recombinant pro-sequence domain and refolded mature enzyme from Escherichia coli by affinity chromatography

Streptococcus pyogenes

667311

recombinant wild-type and inactive mutant enzyme from Escherichia coli by affinity chromatography

Streptococcus pyogenes

668911

the proteins are purified by Ni2+-chelating chromatography with a gradient of 20-200 mM imidazole. The proteins are concentrated by ultrafiltration using a 10 kDa cutoff membrane and then exchanged with phosphate-buffered saline

Streptococcus pyogenes

698880

using Ni-NTA chromatography

Streptococcus pyogenes

733967