This is an abbreviated version!
For detailed information about streptopain, go to the full flat file.

Word Map on EC


preferential cleavage with hydrophobic residues at P2, P1 and P1' =


More, Streptococcus peptidase A, Streptococcus protease, EC, Streptococcal cysteine proteinase, Steptococcus proteinase, proteinase, streptococcal, SCP, Spe B, streptococcal erythrogenic toxin B, streptococcal pyrogenic exotoxin B, SPP, SpeB, streptococcal proteinase, interleukin-1beta convertase, pyrogenic exotoxin B, streptococcal pyrogenic exotoxin B/cysteine protease, SPE B/SCP, SPE B protease, streptococcal cysteine protease, IdeS, IgG-degrading enzyme of Streptococcus pyogenes, SpcCEP, streptococcal pyogenic exotoxin B, streptopain


     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases


Purification on EC - streptopain

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
a combination of two successive strong cation exchange resins gives the best results for soluble, pure enzyme with the highest activity
HiTrap SP column chromatography, and G75 Sephadex gel filtration
native mature Spe B by ammonium sulfate fractionation, dialysis, and ion exchange chromatography, recombinant His-tagged Spe B propeptide and SpeB mutant C47S from Escherichia coli
Ni2+-chelating column chromatography
recombinant His-tagged wild-type and mutant C192S enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the wild-type enzyme autoprocesses during purification to the mature 28 kDa protein
recombinant pro-sequence domain and refolded mature enzyme from Escherichia coli by affinity chromatography
recombinant wild-type and inactive mutant enzyme from Escherichia coli by affinity chromatography
the proteins are purified by Ni2+-chelating chromatography with a gradient of 20-200 mM imidazole. The proteins are concentrated by ultrafiltration using a 10 kDa cutoff membrane and then exchanged with phosphate-buffered saline
using Ni-NTA chromatography