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3.4.22.10: streptopain

This is an abbreviated version!
For detailed information about streptopain, go to the full flat file.

Word Map on EC 3.4.22.10

Reaction

preferential cleavage with hydrophobic residues at P2, P1 and P1' =

Synonyms

EC 3.4.4.18, IdeS, IgG-degrading enzyme of Streptococcus pyogenes, interleukin-1beta convertase, More, proteinase, streptococcal, pyrogenic exotoxin B, SCP, SpcCEP, Spe B, SPE B protease, SPE B/SCP, SpeB, SPP, Steptococcus proteinase, streptococcal cysteine protease, Streptococcal cysteine proteinase, streptococcal erythrogenic toxin B, streptococcal proteinase, streptococcal pyogenic exotoxin B, streptococcal pyrogenic exotoxin B, streptococcal pyrogenic exotoxin B/cysteine protease, Streptococcus peptidase A, Streptococcus protease, streptopain

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.10 streptopain

Cloned

Cloned on EC 3.4.22.10 - streptopain

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C192S mutant expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli
-
expressed in Escherichia coli as a His-tagged fusion protein
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3)pLyS cells
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expression in Escherichia coli, the zymogen proSCP is secreted to the Escherichia coli periplasm followed by subsequent cleavage of the signal peptide
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expression of 12 kDa pro-sequence domain of the enzyme in Escherichia coli, expression of mature enzyme in Escherichia coli in inclusion bodies
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expression of His-tagged wild-type and mutant C192S enzymes in Escherichia coli strain BL21(DE3)
-
expression of mutant C47S
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expression of wild-type and inactive mutant enzyme in Escherichia coli strain BL21(DE3) and in A-549 cells
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gene speB, native expression pattern, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
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genes speB and spi, nucleotide and amino acid sequence determination, analysis and comparison, co-expression of immature Spe B and Spi in Streptococcus pyogenes, Spi is similar to the pro-peptide of Spe B, overview, overexpression of His-tagged Spe B propeptide and SpeB mutant C47S in Escherichia coli
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IdeS GST-fusion protein is expressed in Escherichia coli BL21
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production of large quantities of soluble streptopain using the autoinduction method for Escherichia coli recombinant protein expression with a C-terminal His6 tag. Soluble streptopain is obtained as mature enzyme, indicating that at some point during autoinduction or cell lysis, the protease must have become active. If incubated long enough after lysis, the protease digestes most proteins in the lysate