3.4.21.92: Endopeptidase Clp

This is an abbreviated version!
For detailed information about Endopeptidase Clp, go to the full flat file.

Word Map on EC 3.4.21.92

Reaction

Hydrolysis of proteins to small peptides in the presence of ATP and Mg2+. alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolysed (such as succinyl-Leu-Tyr-/-NHMec; and Leu-Tyr-Leu-/-Tyr-Trp, in which cleavage of the -Tyr-/-Leu- and -Tyr-/-Trp bonds also occurs) =

Synonyms

Protease Ti, ATP-dependent Clp protease, endopeptidase Ti, Caseinolytic protease, ClpP, Clp protease, endopeptidase Clp, Heat shock protein F21.5, stress protein G7, ClpQ, ClpC, ClpA, ClpB, ClpX, ClpY, ClpAP protease, ClpS1, nClpP7, nClpP8, ClpP protease complex, CLP, ClpXP, ClpCP protease, ClpC ATPase, ClpP Protease, ClpP Peptidase, Clp proteolytic subunit, heat-shock protease ClpP, ClpAP, ClpP3, ClpR, ClpP3/R complex, ClpCP3/R protease, ClpE, PfClpP, ClpX2, ATP-dependent caseinolytic protease, ClpP1 protease, ClpP2 protease, ClpXP protease, BsClpP, ClpC1, ATP-dependent Clp protease proteolytic subunit 1, ClpP1, ATP-dependent Clp protease proteolytic subunit 2, ClpP2, CplC, ClpP1P2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.92 Endopeptidase Clp

Storage Stability

Storage Stability on EC 3.4.21.92 - Endopeptidase Clp

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 20% glycerol, 70% loss of activity after 1 month, subunit ClpX
-
-30°C, subunit ClpA, 1 month, 50% loss of activity
-
-30°C, subunit ClpA, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, 20% v/v glycerol, 1 month, 50% loss of activity,
-
-70°C, 50 mM Tris-HCl, pH 7.5, 2 mM EDTA, 2 mM DTT, 10% v/v glycerol, stable for extended periods
-
-70°C, ClpP stable
-
-70°C, stable without substantial loss of activity, subunit ClpX
-
-70°C, subunit ClpP stable
-
4°C, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 0.5 mM EDTA, 10% glycerol, subunit ClpA stable for several months
-
4°C, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, 20% v/v glycerol, 80% loss of activity after 1 week, subunit ClpA
-