3.4.21.92: Endopeptidase Clp

This is an abbreviated version!
For detailed information about Endopeptidase Clp, go to the full flat file.

Word Map on EC 3.4.21.92

Reaction

Hydrolysis of proteins to small peptides in the presence of ATP and Mg2+. alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolysed (such as succinyl-Leu-Tyr-/-NHMec; and Leu-Tyr-Leu-/-Tyr-Trp, in which cleavage of the -Tyr-/-Leu- and -Tyr-/-Trp bonds also occurs) =

Synonyms

Protease Ti, ATP-dependent Clp protease, endopeptidase Ti, Caseinolytic protease, ClpP, Clp protease, endopeptidase Clp, Heat shock protein F21.5, stress protein G7, ClpQ, ClpC, ClpA, ClpB, ClpX, ClpY, ClpAP protease, ClpS1, nClpP7, nClpP8, ClpP protease complex, CLP, ClpXP, ClpCP protease, ClpC ATPase, ClpP Protease, ClpP Peptidase, Clp proteolytic subunit, heat-shock protease ClpP, ClpAP, ClpP3, ClpR, ClpP3/R complex, ClpCP3/R protease, ClpE, PfClpP, ClpX2, ATP-dependent caseinolytic protease, ClpP1 protease, ClpP2 protease, ClpXP protease, BsClpP, ClpC1, ATP-dependent Clp protease proteolytic subunit 1, ClpP1, ATP-dependent Clp protease proteolytic subunit 2, ClpP2, CplC, ClpP1P2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.92 Endopeptidase Clp

Cloned

Cloned on EC 3.4.21.92 - Endopeptidase Clp

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
as His tagged fusion protein
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cloning and mutational analysis of the ATP-binding component ClpA
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Clp sequenced
Clp sequenced, nuclear encoded gene
Clp sequenced, plastid encoded gene
clpP3 and clpR genes cloned into the pACYC Duet vector for co-expression in Escherichia coli BL21-STAR cells
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Escherichia coli DH5alpha transformed with a pEX18Tc-clpP or a pME6031-clpP plasmid by heat shock transformation
expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli using a His-tagged fusion protein
expression in Escherichia coli
exprression in Escherichia coli
into pET-15b vector and expressed as His-tag recombinant protein in Escherichia coli BL21(DE3)pLysS strain
of the ATP-binding subunit ClpA of the ATP-dependent Clp protease
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of the subunit ClpP containing the proteolytic active site
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plasmid encoding ClpPDELTAN transformed into Escherichia coli SG1146GaBL21(DE3) cells
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the entire ClpP gene (amino acid residues 1-196) transferred into pET-22b(+) and expressed in Escherichia coli BL21(DE3)
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