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3.4.21.91: Flavivirin

This is an abbreviated version!
For detailed information about Flavivirin, go to the full flat file.

Word Map on EC 3.4.21.91

Reaction

Selective hydrolysis of -Xaa-Xaa-/-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala =

Synonyms

Dengue NS2b-NS3 protease, Dengue NS3 protease, dengue virus non-structural protein 3, dengue virus NS2B-NS3 protease, dengue virus protease, dengue virus serotype 2 protease, DENV NS2B-NS3, DENV-2 NS2B-NS3 protease, DENV2 NS2BNS3pro, DV2 NS2B/NS3pro, flavivirus NS2B-NS3 protease, non-structural protein 3 serine protease, non-structural protein NS2B-NS3 protease, nonstructural protein 3, NS2-3 protease, NS2B-3 proteinase, NS2B-NS3 complex, NS2B-NS3 protease, NS2B-NS3 protease-helicase, NS2B-NS3 proteinase, NS2B-NS3 proteolytic complex, NS2B-NS3 serine protease, NS2B-NS3 serine proteinase, NS2B-NS3(pro)teinase, NS2b-NS3-protease, NS2B-NS3p, NS2B-NS3pro, NS2B/3 protease, NS2B/NS3, NS2B/NS3 complex, NS2B/NS3 protease, NS2B/NS3 protease complex, NS2B/NS3 proteinase, NS2B/NS3 serine protease, NS2B/NS3pro serine protease, NS2B18NS3, NS2BNS3(pro)tease, NS3, NS3 protease, NS3 proteinase, NS3 serine protease, NS3pro, two-component NS2B-NS3 protease, viral serine protease NS2B/NS3, WNV NS2B-NS3, Yellow fever virus (flavivirus) protease, yellow fever virus NS2B-NS3-181 protease, YFV NS2B/NS3 protease, Zika protease, Zika virus NS2B-NS3 protease, ZIKV NS2B/NS3 protease, ZIKV protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.91 Flavivirin

Purification

Purification on EC 3.4.21.91 - Flavivirin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by anion-exchange chromatography
by immobilized-metal affinity chromatography, ion exchange chromatography and gel filtration
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complete NS3 molecule fused to 18 residues of the NS2B cofactor or NS3 helicase (residues 177 to 618) purified by gel filtration
-
expression and purification of a natural form of DENV protease containing the full-length NS2B protein and the protease domain of NS3. Purified protein in lyso-myristoyl phosphatidylcholine, Fos-14 and Fos15 exhibits protease activity, while almost no activity is observed in Lyso-myristoyl phosphatidylglycerol micelles. The purified protease also exhibits self-cleavage activity when it is purified in lyso-myristoyl phosphatidylcholine micelles, but not in other detergent micelles
-
expression of NS2B(H)-NS3pro complex in E.scherichiacoli predominantly as insoluble protein and purification to 95% purity
-
HiTrap Co2+-chelating column chromatography, gel filtration
-
metal affinity chromatography and gel filtration
-
NS2B-NS3 protease
-
NS2B-NS3pro construct purified by Co2+-chelating Sepharose Fast Flow chromatography
NS2B-NS3pro constructs purified by Co2+ affinity chromatography
NS2B-NS3pro wild-type and K48A constructs purified by Co2+ affinity chromatography and gel filtration, to homogeneity
-
on Ni-NTA column
on Ni2+ affinity column
-
on Ni2+ column
-
protocol for the preparation of the full-length NS2B/NS3 complex from dengue serotype 2 virus by utilizing a Mistic-fusion expression cassette in Escherichia coli. The protease complex is successfully solubilized and stabilized from the bacterial membrane and purified with the use of foscholine-14 detergent. The detergent-solubilized protease complex retains autolytic activity and exists as a robust trimer, implying a molecular assembly in the membrane
-
purified from insoluble inclusion bodies by solubilization in 6 M guanidine hydrochloride and metal chelate resin affinity chromatography
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recombinant DV NS2B-NS3pro, C-terminally tagged with a hexahistidine tag, purified by Co2+ affinity chromatography
-
recombinant His-tagged enzyme complex mutant H51A/S75C from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage via thrombin, dialysis, and gel filtration
recombinant His6-SUMO-tagged linked and unlinked NS3-NS2B(45-95) complex linked from Escherichia coli strain BL21 Codon Plus (DE3) by nickel affinity chromatography, tag cleavage by yeast SUMO Protease 1, followed by another step of nickel affinity chromatography, and gel filtration
recombinant His6-tagged protease DENV2 NS2B-NS3pro linked and form from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant unlinked enzyme is purified by anion exchange chromatography
recombinant separately expressed His-tagged NS2B and NS3pro from Escherichia coli, refolded and forming the active complex, by nickel affinity chromatography, tag cleavage by thrombin and/or TEV protease, and another step of nickel affinity chromatography, and gel filtration
-
to homogeneity
-
wild-type and mutant NS2B-NS3pro and NS2B-NS3prohel constructs, C-terminally tagged with a hexahistidine tag, purified by Co2+ affinity chromatography
-