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11.2
A4-mutant trypsinogen
-
37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM enteropeptidase
-
0.4
angiotensin II
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
28.4
benzoyl-Arg ethyl ester
-
-
262
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
0.36 - 1.81
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
4 - 6.9
bovine trypsinogen
-
23.5 - 26.3
formyl-Ala-Phe-Arg-4-nitroanilide
15.4 - 18.2
formyl-Ala-Phe-Lys-4-nitroanilide
24.9
GD4K 2-naphthylamide
-
pH 8.4, 35°C
64.8
GD4K 7-amido-4-methylcoumarin
-
pH 8.4, 35°C
42 - 49
GD4R-4-nitroanilide
17.8 - 115
GDDDDK-2-naphthylamide
122 - 148
GDDDDK-4-nitroanilide
520
Gly-Asp-ASp-Asp-Asp-2-naphthylamide
-
-
4.27 - 121
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
58.38
Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide
at pH 8.0 and 37°C
0.97 - 21.5
glycyl-tetra-L-aspartyl-L-lysine beta-naphthylamide
2.3 - 35.1
human cationic trypsinogen
-
25.17
MLTAEEKAA
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
111
N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester
-
37°C, pH 8.0
53
N2-benzoyl-L-Arg ethyl ester
-
-
2.62
PrAD4KP26
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
-
0.3 - 1.38
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin
129 - 133
thiobenzyl benzyloxy-carbonyl-L-lysinate
108 - 129
thiobenzyl benzyloxycarbonyl-L-lysinate
8.1 - 12
tosyl-Arg methyl ester
12
Tosyl-Lys methyl ester
-
-
15.9
Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly
-
14.1
Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly
-
17
Val-(Ala)3-Asp-Lys-Ile-Val-Gly
-
14.3
Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly
-
14.6
Val-(Asp)3-Ala-Lys-Ile-Val-Gly
-
8
Val-(Asp)3-Glu-Lys-Ile-Val-Gly
-
13.6
Val-(Asp)4-Arg-Ile-Val-Gly
-
20
Val-(Asp)4-Lys-Ile-Val-Gly
-
17.8
Val-Ala-(Asp)3-Lys-Ile-Val-Gly
-
23
Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly
-
28.1
Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly
-
9.1
Val-Asp-(Ala)3-Lys-Ile-Val-Gly
-
14.7
Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly
-
10.3
Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly
-
0.54
WDDKG
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.68
WDDRG
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.36
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
mutant enzyme E136Y, at pH 8.3 and 30°C
0.56
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
mutant enzyme R213L, at pH 8.3 and 30°C
0.64
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
wild type enzyme, at pH 8.3 and 30°C
1
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
mutant enzyme H24Q, at pH 8.3 and 30°C
1.81
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
mutant enzyme E136Y/R213L, at pH 8.3 and 30°C
4
bovine trypsinogen
-
21 °C, 50 mM sodium citrate pH 5.6, 1 nM enteropeptidase
-
6.9
bovine trypsinogen
-
37 °C, 25 mM Tris-HCl pH 8.4, 10 mM CaCl2, 0. 4 mM ovomucoid, 0.3 nM enteropeptidase
-
23.5
formyl-Ala-Phe-Arg-4-nitroanilide
-
pH 8.4, 25°C
26.3
formyl-Ala-Phe-Arg-4-nitroanilide
-
pH 8.4, 25°C
15.4
formyl-Ala-Phe-Lys-4-nitroanilide
-
pH 8.4, 25°C
18.2
formyl-Ala-Phe-Lys-4-nitroanilide
-
pH 8.4, 25°C
0.97
G5DKF(NO2)G
-
50 mM Ca2+, pH 8.0, 37°C
0.97
G5DKF(NO2)G
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
17.33
G5DKF(NO2)G
-
50 mM Ca2+, pH 8.0, 37°C
17.33
G5DKF(NO2)G
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.99
GD4KF(NO2)G
-
50 mM Ca2+, pH 8.0, 37°C
0.99
GD4KF(NO2)G
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
17.83
GD4KF(NO2)G
-
50 mM Ca2+, pH 8.0, 37°C
17.83
GD4KF(NO2)G
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.97
GD4KNfa
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
16.67
GD4KNfa
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
42
GD4R-4-nitroanilide
mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C
45
GD4R-4-nitroanilide
wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C
49
GD4R-4-nitroanilide
mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C
17.8
GDDDDK-2-naphthylamide
mutant enzyme E136Y, at pH 8.3 and 30°C
26.2
GDDDDK-2-naphthylamide
mutant enzyme H24Q, at pH 8.3 and 30°C
27.9
GDDDDK-2-naphthylamide
wild type enzyme, at pH 8.3 and 30°C
28.75
GDDDDK-2-naphthylamide
mutant enzyme E136Y/R213L, at pH 8.3 and 30°C
33.02
GDDDDK-2-naphthylamide
mutant enzyme R213L, at pH 8.3 and 30°C
92
GDDDDK-2-naphthylamide
-
soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C
115
GDDDDK-2-naphthylamide
-
phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C
122
GDDDDK-4-nitroanilide
mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C
135
GDDDDK-4-nitroanilide
wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C
148
GDDDDK-4-nitroanilide
mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C
4.27
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
-
-
17.1
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
-
mutant enzyme K96A
20.8
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
-
pH 8.0, 25°C
24.1
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
-
-
25.5
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
-
mutant enzyme K97A
39.1
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
-
mutant enzyme K98A
49.3
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
-
pH 8.4, 37°C
98
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
value obtained by fluorescence spectroscopy, pH not specified in the publication, at 37°C
115
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
pH 8.4, 37°C
118
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
-
pH 8.4, 37°C
121
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
value obtained by impedance spectroscopy, pH not specified in the publication, at 37°C
0.97
glycyl-tetra-L-aspartyl-L-lysine beta-naphthylamide
-
50 mM Ca2+, pH 8.0, 37°C
16.67
glycyl-tetra-L-aspartyl-L-lysine beta-naphthylamide
-
50 mM Ca2+, pH 8.0, 37°C
21.5
glycyl-tetra-L-aspartyl-L-lysine beta-naphthylamide
-
pH 8.0, 37°C
2.3
human cationic trypsinogen
-
25°C, 28 mM sodium succinate (pH 5.6), 10 mM CaCl2
-
2.3
human cationic trypsinogen
-
0.00036 1/sec/mg, incubates 30 min at 37°C, pH 9.0, spectrophotometrically measured at 415 nm
-
8.8
human cationic trypsinogen
-
37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.45 nM enteropeptidase
-
35.1
human cationic trypsinogen
-
37 °C, 100 mM Tris-HCl pH 8.0, 1mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM enteropeptidase
-
0.07
LTAEEKA
-
pH 8.0, 37°C
0.49
LTAEEKA
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.3
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin
mutant enzyme E136Y, at pH 8.3 and 30°C
0.34
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin
wild type enzyme, at pH 8.3 and 30°C
0.53
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin
mutant enzyme R213L, at pH 8.3 and 30°C
0.6
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin
mutant enzyme H24Q, at pH 8.3 and 30°C
1.38
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin
mutant enzyme E136Y/R213L, at pH 8.3 and 30°C
129
thiobenzyl benzyloxy-carbonyl-L-lysinate
-
pH 8.4, 25°C
133
thiobenzyl benzyloxy-carbonyl-L-lysinate
pH 8.4, 37°C
133
thiobenzyl benzyloxy-carbonyl-L-lysinate
-
pH 8.4, 25°C
108
thiobenzyl benzyloxycarbonyl-L-lysinate
-
mutant enzyme K96A
120
thiobenzyl benzyloxycarbonyl-L-lysinate
-
mutant enzyme R99A
128
thiobenzyl benzyloxycarbonyl-L-lysinate
-
mutant enzyme R97A
128
thiobenzyl benzyloxycarbonyl-L-lysinate
-
mutant enzyme R98A
129
thiobenzyl benzyloxycarbonyl-L-lysinate
-
-
8.1
tosyl-Arg methyl ester
-
-
12
tosyl-Arg methyl ester
-
-
0.005
Trypsinogen
-
bovine guanidinated trypsinogen
1.48
Trypsinogen
-
bovine trypsinogen
2.3
Trypsinogen
-
bovine trypsinogen
2.8
Trypsinogen
-
human trypsinogen
4.8
Trypsinogen
-
bovine trypsinogen
319
Z-Lys-SBzl
mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C
336
Z-Lys-SBzl
wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C
354
Z-Lys-SBzl
mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C