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3.4.21.9: enteropeptidase

This is an abbreviated version!
For detailed information about enteropeptidase, go to the full flat file.

Word Map on EC 3.4.21.9

Reaction

Activation of trypsinogen by selective cleavage of Lys6-/-Ile bond =

Synonyms

BEK, BEP, bovine enterokinase light chain, bovine enteropeptidase, Chinese bovine enterokinase, Chinese northern yellow bovine enterokinase catalytic subunit, EC 3.4.4.8, EK, EKL, EKL-His6, EKLC, enterokinase, enterokinase light chain, enteropeptidase, enteropeptidase light chain, EP 118-1035, EP-1, EPL, HEK, HEP, human enteropeptidase, L-BEP, L-HEK, L-HEP, native enterokinase, natural enteropeptidase, peptidase, entero-, porcine enterokinase, PRSS7, recombinant bovine enterokinase catalytic subunit protein, recombinant enterokinase light chain, recombinant His-tagged enterokinase light chain, rEKL, rEKL/His, sBEKLC, TMPRSS15

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.9 enteropeptidase

Reference

Reference on EC 3.4.21.9 - enteropeptidase

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Grant, D.A.W.; Hermon-Taylor, J.
Enterokinase
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
5
143-155
1984
Bos taurus, Homo sapiens, Sus scrofa
-
Manually annotated by BRENDA team
Light, A.; Fonseca, P.
The preparation and properties of the catalytic subunit of bovine enterokinase
J. Biol. Chem.
259
13195-13198
1984
Bos taurus
Manually annotated by BRENDA team
Fonseca, P.; Light, A.
The purification and characterization of bovine enterokinase from membrane fragments in the duodenal mucosal fluid
J. Biol. Chem.
258
14516-14520
1983
Bos taurus
Manually annotated by BRENDA team
Magee, A.I.; Grant, D.A.W.; Hermon-Taylor, J.; Offord, R.E.
Specific one-stage method for assay of enterokinase activity by release of radiolabelled activation peptides from alpha-N-[3H]acetyl-trypsinogen and the effect of calcium ions on the enzyme activity
Biochem. J.
197
239-244
1981
Homo sapiens
Manually annotated by BRENDA team
Light, A.; Savithri, H.S.; Liepnieks, J.J.
Specificity of bovine enterokinase toward protein substrates
Anal. Biochem.
106
199-206
1980
Bos taurus
Manually annotated by BRENDA team
Liepnieks, J.J.; Light, A.
The preparation and properties of bovine enterokinase
J. Biol. Chem.
254
1677-1683
1979
Bos taurus
Manually annotated by BRENDA team
Brodrick, J.W.; Largman, C.; Hsiang, M.W.; Johnson, J.H.; Geokas, M.C.
Structural basis for the specific activation of human enteropeptidase
J. Biol. Chem.
253
2737-2742
1978
Homo sapiens, Sus scrofa
Manually annotated by BRENDA team
Anderson, L.E.; Walsh, K.A.; Neurath, H.
Bovine enterokinase. Purification, specificity, and some molecular properties
Biochemistry
16
3354-3360
1977
Bos taurus
Manually annotated by BRENDA team
Grant, D.A.W.; Hermon-Taylor, J.
The purification of human enterokinase by affinity chromatography and immunoadsorption. Some observations on its molecular characteristics and comparisons with the pig enzyme
Biochem. J.
155
243-254
1976
Homo sapiens, Sus scrofa
Manually annotated by BRENDA team
Baratti, J.; Maroux, S.; Louvard, D.; Desnuelle, P.
On porcine enterokinase. Further purification and some molecular properties
Biochim. Biophys. Acta
315
147-161
1973
Sus scrofa
Manually annotated by BRENDA team
Maroux, S.; Baratti, J.; Desnuelle, P.
Purification and specificity of porcine enterokinase
J. Biol. Chem.
246
5031-5039
1971
Sus scrofa
Manually annotated by BRENDA team
Xin, Y.; Zheng, X.; Lu, D.; Rubin, D.C.; Pung, C.Y.M.; Sadler, J.E.
Structure of murine enterokinase (enteropeptidase) and expression in small intestine during development
Gastrointest. Liver Physiol.
37
G342-G349
1998
Mus musculus
-
Manually annotated by BRENDA team
Lu, D.; Futterer, K.; Korolev, S.; Zheng, X.; Tan, K.; Waksman, G.; Sadler, J.E.
Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide
J. Mol. Biol.
292
361-373
1999
Bos taurus
Manually annotated by BRENDA team
Lu, D.; Yua, X.; Zheng, X.; Sadler, J.E.
Bovine proenteropeptidase is activated by trypsin, and the specificity of enteropeptidase depends on the heavy chain
J. Biol. Chem.
272
31293-31300
1997
Bos taurus
Manually annotated by BRENDA team
Miyoshi, Y.; Onishi, T.; Sano, T.; Komi, N.
Monoclonal antibody against human enterokinase and immunohistochemica localization of the enzyme
Gastroenterol. Jpn.
25
320-327
1990
Homo sapiens
Manually annotated by BRENDA team
Mann, N.S.; Mann, S.K.
Enterokinase
Proc. Soc. Exp. Biol. Med.
206
114-118
1994
Homo sapiens
Manually annotated by BRENDA team
Naude, R.J.; da Silva, D.; Egde, W.; Oelofsen, W.
The isolation and partial characterization of enterokinase from ostrich (Struthio camelus) duodenal mucosa
Comp. Biochem. Physiol. B
105
591-595
1993
Struthio camelus
-
Manually annotated by BRENDA team
Light, A.; Janska, G.
Enterokinase (enteropeptidase): comparative aspects
Trends Biochem. Sci.
14
110-112
1989
Bos taurus, Homo sapiens, Sus scrofa
Manually annotated by BRENDA team
Vozza, L.A.; Wittwer, L.; Higgins, D.R.; Purcell, T.J.; Bergseid, M.; Collins-Racie, L.A.; LaVallie, E.R.; Hoeffler, J.P.
Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris
Biotechnology
14
77-81
1996
Bos taurus
Manually annotated by BRENDA team
Matsushima, M.; Ichinose, M.; Yahagi, N.; Tsukada-Kato, S.; Miki, K.; Omata, M.; Kim, Y.T.; Ito, H.; Takahashi, T.; Sakurai, Y.; Tsuchiya, Y.; Athauda, S.B.P.; Inoue, H.; Takahashi, K.
Purification and further characterization of enteropeptidase from porcine duodenum
J. Biochem.
125
947-951
1999
Sus scrofa
Manually annotated by BRENDA team
Collins-Racie, L.A.; McColgan, J.M.; Grant, K.L.; DiBlasio-Smith, A.; McCoy, J.M.; LaVallie, E.R.
Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using novel secretory fusion partner DsbA
Biotechnology
13
982-987
1995
Bos taurus
Manually annotated by BRENDA team
Yuan, L.D.; Hua, Z.C.
Expression, purification, and characterization of a biologically active bovine enterokinase catalytic subunit in Escherichia coli
Protein Expr. Purif.
25
300-304
2002
Bos taurus
Manually annotated by BRENDA team
Gasparian, M.E.; Ostapchenko, V.G.; Schulga, A.A.; Dolgikh, D.A.; Kirpichnikov, M.P.
Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli
Protein Expr. Purif.
31
133-139
2003
Homo sapiens (P98073), Homo sapiens
Manually annotated by BRENDA team
Fang, L.; Sun, Q.M.; Hua, Z.C.
Expression of recombinant chinese bovine enterokinase catalytic subunit in P. pastoris and its purification and characterization
Acta Biochim. Biophys. Sin.
36
513-517
2004
Bos taurus
Manually annotated by BRENDA team
Mikhailova, A.G.; Likhareva, V.V.; Vaskovsky, B.V.; Garanin, S.K.; Onoprienko, L.V.; Prudchenko, I.A.; Chikin, L.D.; Rumsh, L.D.
Study of secondary specificity of enteropeptidase in comparison with trypsin
Biochemistry (Moscow)
69
909-917
2004
Bos taurus
Manually annotated by BRENDA team
Mikhailova, A.G.; Likhareva, V.V.; Prudchenko, I.A.; Rumsh, L.D.
Effect of calcium ions on enteropeptidase catalysis
Biochemistry (Moscow)
70
1129-1135
2005
Homo sapiens
Manually annotated by BRENDA team
Huang, H.; Zhao, Y.; Yi-ru, G.
Prokaryotic expression of Chinese bovine enterokinase catalytic subunit
Chin. Med. Sci.
117
286-290
2004
Bos taurus
Manually annotated by BRENDA team
Nemoda, Z.; Sahin-Toth, M.
The tetra-aspartate motif in the activation peptide of human cationic trypsinogen is essential for autoactivation control but not for enteropeptidase recognition
J. Biol. Chem.
280
29645-29652
2005
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Peng, L.; Zhong, X.; Ou, J.; Zheng, S.; Liao, J.; Wang, L.; Xu, A.
High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris
J. Biotechnol.
108
185-192
2004
Bos taurus
Manually annotated by BRENDA team
Liew, O.W.; Ching Chong, J.P.; Yandle, T.G.; Brennan, S.O.
Preparation of recombinant thioredoxin fused N-terminal proCNP: Analysis of enterokinase cleavage products reveals new enterokinase cleavage sites
Protein Expr. Purif.
41
332-340
2005
Sus scrofa
Manually annotated by BRENDA team
Gasparian, M.E.; Ostapchenko, V.G.; Dolgikh, D.A.; Kirpichnikov, M.P.
Biochemical characterization of human enteropeptidase light chain
Biochemistry (Moscow)
71
113-119
2006
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Liew, O.W.; Jenny Chong, P.C.; Lim, Y.Z.; Ang, C.X.; Amy Lau, Y.C.; Yandle, T.G.; Brennan, S.O.
An SRLLR motif downstream of the scissile bond enhances enterokinase cleavage efficiency
Biochimie
89
21-29
2007
Sus scrofa
Manually annotated by BRENDA team
Kubitzki, T.; Noll, T.; Luetz, S.
Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage
Bioprocess Biosyst. Eng.
31
173-182
2008
Bos taurus
Manually annotated by BRENDA team
Gasparian, M.E.; Ostapchenko, V.G.; Yagolovich, A.V.; Tsygannik, I.N.; Chernyak, B.V.; Dolgikh, D.A.; Kirpichnikov, M.P.
Overexpression and refolding of thioredoxin/TRAIL fusion from inclusion bodies and further purification of TRAIL after cleavage by enteropeptidase
Biotechnol. Lett.
29
1567-1573
2007
Homo sapiens
Manually annotated by BRENDA team
Huang, L.; Ruan, H.; Gu, W.; Xu, Z.; Cen, P.; Fan, L.
Functional expression and purification of bovine enterokinase light chain in recombinant Escherichia coli
Prep. Biochem. Biotechnol.
37
205-217
2007
Bos taurus
Manually annotated by BRENDA team
Boulware, K.T.; Daugherty, P.S.
Protease specificity determination by using cellular libraries of peptide substrates (CLiPS)
Proc. Natl. Acad. Sci. USA
103
7583-7588
2006
Homo sapiens
Manually annotated by BRENDA team
Tan, H.; Wang, J.; Zhao, Z.K.
Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli
Protein Expr. Purif.
56
40-47
2007
Bos taurus (Q6B4R4), Bos taurus
Manually annotated by BRENDA team
Mikhailova, A.G.; Likhareva, V.V.; Teich, N.; Rumsh, L.D.
The ways of realization of high specificity and efficiency of enteropeptidase
Protein Pept. Lett.
14
227-232
2007
Homo sapiens, Bos taurus (Q6B4R4), Bos taurus
Manually annotated by BRENDA team
Kim, Y.T.; Nishii, W.; Matsushima, M.; Inoue, H.; Ito, H.; Park, S.J.; Takahashi, K.
Substrate specificities of porcine and bovine enteropeptidases toward the peptide Val-(Asp)4-Lys-Ile-Val-Gly and its analogs
Biosci. Biotechnol. Biochem.
72
905-908
2008
Bos taurus (P98072), Sus scrofa (P98074)
Manually annotated by BRENDA team
Shahravan, S.H.; Qu, X.; Chan, I.S.; Shin, J.A.
Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tag
Protein Expr. Purif.
59
314-319
2008
synthetic construct
Manually annotated by BRENDA team
Kubitzki, T.; Minoer, D.; Mackfeld, U.; Oldiges, M.; Noll, T.; Luetz, S.
Application of immobilized bovine enterokinase in repetitive fusion protein cleavage for the production of mucin 1
Biotechnol. J.
4
1610-1618
2009
Bos taurus
Manually annotated by BRENDA team
Ogiwara, K.; Takahashi. T.
Specificity of the medaka enteropeptidase serine protease and its usefulness as a biotechnological tool for fusion-protein cleavage
Proc. Natl. Acad. Sci. USA
104
7021-7026
2007
Oryzias latipes (A4UWM5), Oryzias latipes
Manually annotated by BRENDA team
Makarova, A.M.; Gorbacheva, L.R.; Savinkova, I.V.; Mikhailova, A.G.; Rumsh, L.D.; Pinelis, V.G.; Strukova, S.M.
Effect of enteropeptidase on survival of cultured hippocampal neurons under conditions of glutamate toxicity
Biochemistry (Moscow)
75
1153-1159
2010
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Chun, H.; Joo, K.; Lee, J.; Shin, H.C.
Design and efficient production of bovine enterokinase light chain with higher specificity in E. coli
Biotechnol. Lett.
33
1227-1232
2011
Bos taurus
Manually annotated by BRENDA team
Choi, M.G.; Lee, E.; Chung, H.S.; Jang, S.H.; Lee, C.
A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin
BMB Rep.
44
458-461
2011
Homo sapiens
Manually annotated by BRENDA team
Pepeliaev, S.; Krahulec, J.; ?erny, Z.; Jilkova, J.; Tlusta, M.; Dostalova, J.
High level expression of human enteropeptidase light chain in Pichia pastoris
J. Biotechnol.
156
67-75
2011
Homo sapiens (P98073), Homo sapiens
Manually annotated by BRENDA team
Shirokov, D.; Ryabichenko, V.; Akishina, R.; Ospelnikova, T.; Glazunov, A.; Chestukhina, G.; Veiko, V.
Development of hybrid human interferon alfa-2 strain-producers and the use of enteropeptidase for production of N-terminal methionine-free interferons
Mol. Biol.
45
466-471
2011
Sus scrofa
Manually annotated by BRENDA team
Simeonov, P.; Berger-Hoffmann, R.; Hoffmann, R.; Straeter, N.; Zuchner, T.
Surface supercharged human enteropeptidase light chain shows improved solubility and refolding yield
Protein Eng. Des. Sel.
24
261-268
2011
Homo sapiens (P98073), Homo sapiens
Manually annotated by BRENDA team
Gasparian, M.E.; Bychkov, M.L.; Dolgikh, D.A.; Kirpichnikov, M.P.
Strategy for improvement of enteropeptidase efficiency in tag removal processes
Protein Expr. Purif.
79
191-196
2011
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Gasparian, M.E.; Bobik, T.V.; Kim, Y.V.; Ponomarenko, N.A.; Dolgikh, D.A.; Gabibov, A.G.; Kirpichnikov, M.P.
Heterogeneous catalysis on the phage surface: Display of active human enteropeptidase
Biochimie
95
2076-2081
2013
Homo sapiens
Manually annotated by BRENDA team
Ostapchenko, V.G.; Gasparian, M.E.; Kosinsky, Y.A.; Efremov, R.G.; Dolgikh, D.A.; Kirpichnikov, M.P.
Dissecting structural basis of the unique substrate selectivity of human enteropeptidase catalytic subunit
J. Biomol. Struct. Dyn.
30
62-73
2012
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Jirickova, K.; Gal, M.; Krahulec, J.; Hives, J.
Electrochemical determination of basic biochemical properties of enzyme enterokinase
Monatsh. Chem.
146
755-759
2015
Homo sapiens (P98073)
-
Manually annotated by BRENDA team
Prohaska, T.A.; Wahlmueller, F.C.; Furtmueller, M.; Geiger, M.
Interaction of protein C inhibitor with the type II transmembrane serine protease enteropeptidase
PLoS ONE
7
e39262
2012
Homo sapiens
Manually annotated by BRENDA team
Azhar, M.; Somashekhar, R.
Production and purification of recombinant enteropeptidase expressed in an insect-baculovirus cell system
Prep. Biochem. Biotechnol.
45
268-278
2015
Bos taurus (R4QR01), Bos taurus
Manually annotated by BRENDA team
Xu, J.; Hu, S.; Wang, X.; Zhao, Z.; Zhang, X.; Wang, H.; Zhang, D.; Guo, Y.
Structure basis for the unique specificity of medaka enteropeptidase light chain
Protein Cell
5
178-181
2014
Oryzias latipes (A4UWM5), Oryzias latipes
Manually annotated by BRENDA team
Smith, E.T.; Johnson, D.A.
Human enteropeptidase light chain: bioengineering of recombinants and kinetic investigations of structure and function
Protein Sci.
22
577-585
2013
Homo sapiens (P98073), Homo sapiens
Manually annotated by BRENDA team
Simeonov, P.; Zahn, M.; Straeter, N.; Zuchner, T.
Crystal structure of a supercharged variant of the human enteropeptidase light chain
Proteins
80
1907-1910
2012
Homo sapiens (P98073), Homo sapiens
Manually annotated by BRENDA team
Melicherova, K.; Krahulec, J.; Safranek, M.; Liskova, V.; Hopkova, D.; Szeliova, D.; Turna, J.
Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris
Appl. Microbiol. Biotechnol.
101
1927-1934
2017
Homo sapiens (P98073), Homo sapiens
Manually annotated by BRENDA team
Niu, L.; Li, J.; Ji, X.; Yang, B.
Efficient expression and purification of recombinant human enteropeptidase light chain in Escherichia coli
Braz. Arch. Biol. Technol.
58
216-221
2015
Homo sapiens (P98073)
-
Manually annotated by BRENDA team
Wang, J.H.; Tang, M.Z.; Yu, X.T.; Xu, C.M.; Yang, H.M.; Tang, J.B.
Site-specific, covalent immobilization of an engineered enterokinase onto magnetic nanoparticles through transglutaminase-catalyzed bioconjugation
Colloids Surf. B Biointerfaces
177
506-511
2019
Bos taurus
Manually annotated by BRENDA team
Hayashi, H.; Kubo, Y.; Izumida, M.; Takahashi, E.; Kido, H.; Sato, K.; Yamaya, M.; Nishimura, H.; Nakayama, K.; Matsuyama, T.
Enterokinase enhances influenza A virus infection by activating trypsinogen in human cell lines
Front. Cell. Infect. Microbiol.
8
91
2018
Homo sapiens (P98073), Homo sapiens
Manually annotated by BRENDA team