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3.4.21.9: enteropeptidase

This is an abbreviated version!
For detailed information about enteropeptidase, go to the full flat file.

Word Map on EC 3.4.21.9

Reaction

Activation of trypsinogen by selective cleavage of Lys6-/-Ile bond =

Synonyms

BEK, BEP, bovine enterokinase light chain, bovine enteropeptidase, Chinese bovine enterokinase, Chinese northern yellow bovine enterokinase catalytic subunit, EC 3.4.4.8, EK, EKL, EKL-His6, EKLC, enterokinase, enterokinase light chain, enteropeptidase, enteropeptidase light chain, EP 118-1035, EP-1, EPL, HEK, HEP, human enteropeptidase, L-BEP, L-HEK, L-HEP, native enterokinase, natural enteropeptidase, peptidase, entero-, porcine enterokinase, PRSS7, recombinant bovine enterokinase catalytic subunit protein, recombinant enterokinase light chain, recombinant His-tagged enterokinase light chain, rEKL, rEKL/His, sBEKLC, TMPRSS15

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.9 enteropeptidase

KM Value

KM Value on EC 3.4.21.9 - enteropeptidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0021
A4-mutant trypsinogen
-
37 °C, 100 mM Tris-HCl (pH 8.0), 1 mM CaCl2, 120 nM soybean trypsin inhibitor, 0.13 nM human enteropeptidase
-
3.3
angiotensin II
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.0647 - 1.41
APFDDDDKIVGG
0.147
APFDDDDRIVGG
at 0.014-0.05 mM, holoenzyme
0.3
APFDDDGKIVGG
at 0.08-2 mM, holoenzyme
1.24
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
0.5 - 1.6
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
0.2 - 1.3
Boc-Glu(OBzl)-Ala-Arg-MCA
1.2 - 5.6
bovine trypsinogen
-
0.076 - 0.192
formyl-Ala-Phe-Arg-4-nitroanilide
0.167 - 0.28
formyl-Ala-Phe-Lys-4-nitroanilide
0.437
G5DKF(NO2)G
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.5 - 0.6
GD4K 2-naphthylamide
-
pH 8.4, 35°C
0.025
GD4K 7-amido-4-methylcoumarin
-
pH 8.4, 35°C
0.16
GD4KF(NO2)G
0.2
GD4KNfa
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.00258 - 0.018
GD4R-4-nitroanilide
0.019 - 0.6
GDDDDK-2-naphthylamide
0.0179 - 0.276
GDDDDK-4-nitroanilide
0.75
Gly-(L-Asp)4-L-Lys-2-naphthylamide
pH and temperature not specified in the publication
1.12
Gly-Asp-ASp-Asp-Asp-2-naphthylamide
-
-
0.034 - 1.25
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide
0.034 - 0.332
Gly-Asp-Asp-Asp-Asp-Lys-naphthylamide
0.108
Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide
at pH 8.0 and 37°C
0.2 - 0.525
glycyl-tetra-L-aspartyl-L-lysine beta-naphthylamide
0.0014 - 1.5
human cationic trypsinogen
-
4.2
LTAEEKA
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
4
MLTAEEKAA
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
596
N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester +
-
37°C, pH 8.0
-
0.076
N-formyl-Ala-Phe-Arg-4-nitroanilide
-
-
0.167
N-formyl-Ala-Phe-Lys-4-nitroanilide
-
-
0.125
PrAD4KP26
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
-
1 - 10
Pro-Phe-Arg-4-methylcoumaryl-7-amide
0.2 - 1.5
t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin
0.12 - 0.14
thiobenzyl benzyloxy-carbonyl-L-lysinate
0.05 - 0.14
thiobenzyl benzyloxycarbonyl-L-lysinate
0.001 - 1.2
Trypsinogen
0.72
Val-(Ala)2-(Asp)2-Lys-Ile-Val-Gly
-
8.77
Val-(Ala)2-Asp-Ala-Lys-Ile-Val-Gly
-
1.51
Val-(Ala)3-Asp-Lys-Ile-Val-Gly
-
1.25
Val-(Asp)2-Ala-Asp-Lys-Ile-Val-Gly
-
8.31
Val-(Asp)3-Ala-Lys-Ile-Val-Gly
-
0.8
Val-(Asp)3-Glu-Lys-Ile-Val-Gly
-
0.45
Val-(Asp)4-Arg-Ile-Val-Gly
-
1.77
Val-(Asp)4-Lys-Ile-Val-Gly
-
1.01
Val-Ala-(Asp)3-Lys-Ile-Val-Gly
-
3.39
Val-Ala-Asp-Ala-Asp-Lys-Ile-Val-Gly
-
1.24
Val-Asp-(Ala)2-Asp-Lys-Ile-Val-Gly
-
21.11
Val-Asp-(Ala)3-Lys-Ile-Val-Gly
-
0.76
Val-Asp-Ala-(Asp)2-Lys-Ile-Val-Gly
-
5.57
Val-Asp-Ala-Asp-Ala-Lys-Ile-Val-Gly
-
1.6
WDDKG
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
2.1
WDDRG
-
in 0.1 M Tris-HCl buffer pH 8.0 at 37°C
0.356
Z-Ala-Ala-Arg-nitroanilide
-
-
0.234
Z-Ala-Leu-Arg-nitroanilide
-
-
0.42
Z-Ala-Met-Arg-nitroanilide
-
-
0.067
Z-Ala-Phe-Arg-nitroanilide
-
-
0.24
Z-Ala-Trp-Arg-nitroanilide
-
-
0.109
Z-Ala-Tyr-Arg-nitroanilide
-
-
0.091
Z-Gly-Pro-Arg-nitroanilide
-
-
0.165 - 0.19
Z-Lys-SBzl
0.1 - 0.4
Z-Phe-Arg-4-methylcoumaryl-7-amide
0.265
Z-Pro-Phe-Arg-nitroanilide
-
-