3.4.21.83: Oligopeptidase B
This is an abbreviated version!
For detailed information about Oligopeptidase B, go to the full flat file.
Word Map on EC 3.4.21.83
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3.4.21.83
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gingivalis
-
prolyl
-
leupeptin
-
chymotrypsin-like
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antipain
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porphyromonas
-
aprotinin
-
chloromethyl
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chymostatin
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tlck
-
tryptase
-
2-macroglobulin
-
benzamidine
-
acrosin
-
phenylmethanesulfonyl
-
proteamaculans
-
bapna
-
drug development
-
medicine
- 3.4.21.83
- gingivalis
-
prolyl
- leupeptin
-
chymotrypsin-like
- antipain
- porphyromonas
- aprotinin
-
chloromethyl
- chymostatin
- tlck
- tryptase
-
2-macroglobulin
- benzamidine
- acrosin
-
phenylmethanesulfonyl
- proteamaculans
-
bapna
- drug development
- medicine
Reaction
Hydrolysis of -Arg-/-, -Lys-/- bonds in oligopeptides, even when P1' residue is proline =
Synonyms
La_OpB, oligopeptidase B, oligopeptidase B-like, oligopeptidase B2, OP-Tb, OPB, OPB2, OPBTc, Opd B, OpdB, Protease II, Proteinase, Escherichia coli alkaline, II, PSP, Spro_3467, Tb-OP, Tc 120, Tc-OP, trypsin-like protease
ECTree
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Temperature Stability
Temperature Stability on EC 3.4.21.83 - Oligopeptidase B
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30
37
40
42
45
45 - 53
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following treatment at 45°C for 20 min the relative activity of wild type enzyme is 89%, the melting temperature of the wild type enzyme is at 53.3°C
50
fluorescence intensity of enzyme decreases with increasing heat treatment time and the maximum fluorescence wavelength shifts slightly towards longer wavelength, 340-345 nm and 342-346 nm. The fluorescence intensity in 50% glycerol does not change significantly following heat treatment. The maximum fluorescence wavelength also shifts slightly, 337-340 nm and 339-340 nm
37
wild-type and mutant PSP enzymes can undergo reversible thermal inactivation at 37°C, pH 8.0, with activity being restored or even increased with respect to the initial one upon subsequent cooling. The process can be repeated several times, with the same results achieved (up to 5 cycles). This effect can be explained by a shift in the equilibrium between the inactive open form of the enzyme and the active closed one upon variation of the incubation temperature, overview
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30 min, wild-type, 89% residual activity, mutants E172Q, E490Q, E524Q and R689A 99%, 9%, 49% and 73% residual activity, respectively