3.4.21.83: Oligopeptidase B

This is an abbreviated version!
For detailed information about Oligopeptidase B, go to the full flat file.

Word Map on EC 3.4.21.83

Reaction

Hydrolysis of -Arg-/-, -Lys-/- bonds in oligopeptides, even when P1' residue is proline =

Synonyms

Proteinase, Escherichia coli alkaline, II, Protease II, PSP, OpdB, Tb-OP, Tc-OP, oligopeptidase B, La_OpB, OP-Tb, Opd B, Tc 120, OPB, trypsin-like protease, OPBTc, Spro_3467, oligopeptidase B2, oligopeptidase B-like, OPB2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.83 Oligopeptidase B

Cloned

Cloned on EC 3.4.21.83 - Oligopeptidase B

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli HB101 gene cloned and expressed in Escherichia coli JM83
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expressed in Escherichia coli
expressed in Escherichia coli BL-21(DE3) cells
expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells
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expression in Escherichia coli
fusion expression of oligopeptidase B with an N-terminal histidine tag using pET28a as the expression vector. Although most of the recombinant OpdB is produced as inclusion bodies, the solubility of the recombinant protease increases significantly when expression temperature shifts from 37°C to 30°C
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location of the protease II gene on the physical map of the Escherichia coli chromosome
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Oligopeptidase B is overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein
opdB gene PCR amplified and expressed in Escherichia coli BL21(lambdaDE3)
overexpressed in Escherichia coli
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overexpressed in Escherichia coli JM83
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recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)