Oligopeptidase B

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For detailed information about Oligopeptidase B, go to the full flat file.

Word Map on EC


Hydrolysis of -Arg-/-, -Lys-/- bonds in oligopeptides, even when P1' residue is proline =


Proteinase, Escherichia coli alkaline, II, Protease II, PSP, OpdB, Tb-OP, Tc-OP, oligopeptidase B, La_OpB, OP-Tb, Opd B, Tc 120, OPB, trypsin-like protease, OPBTc, Spro_3467, oligopeptidase B2, oligopeptidase B-like, OPB2


     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
       Oligopeptidase B


Cloned on EC - Oligopeptidase B

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CLONED (Commentary)
Escherichia coli HB101 gene cloned and expressed in Escherichia coli JM83
expressed in Escherichia coli
expressed in Escherichia coli BL-21(DE3) cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells
expression in Escherichia coli
fusion expression of oligopeptidase B with an N-terminal histidine tag using pET28a as the expression vector. Although most of the recombinant OpdB is produced as inclusion bodies, the solubility of the recombinant protease increases significantly when expression temperature shifts from 37°C to 30°C
location of the protease II gene on the physical map of the Escherichia coli chromosome
Oligopeptidase B is overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein
opdB gene PCR amplified and expressed in Escherichia coli BL21(lambdaDE3)
overexpressed in Escherichia coli
overexpressed in Escherichia coli JM83
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)