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3.4.21.79: granzyme B

This is an abbreviated version!
For detailed information about granzyme B, go to the full flat file.

Word Map on EC 3.4.21.79

Reaction

preferential cleavage: -Asp-/- >> -Asn-/- > -Met-/-, -Ser-/- =

Synonyms

Asp-ase, C11, CCP1, CCPII, CTLA1, CTSGL1, Cytotoxic cell proteinase-1, cytotoxic lymphocyte-associated protease, cytotoxic lymphocyte-specific protein, cytotoxic serine protease granzyme B, cytotoxic T-lymphocyte-associated gene transcript-1, gB, Gra-b, granzyme B, Granzyme G, Granzyme H, GrB, GrzmB, GzB, Gzm, Gzm B, GzmB, GzmB-like enzyme, GzmH, HLp, Human lymphocyte protein, Lymphocyte protease, natural killer cell protease 1, pro-apoptotic serine protease, proGrB, Proteinase, CCP1, rat grB[N66Q], SECT, T-cell serine protease 1-3E

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.79 granzyme B

Cloned

Cloned on EC 3.4.21.79 - granzyme B

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
293TT cells transiently transfected with plasmids encoding either for wild-type or mutant GrzmB. cDNA subcloned into the mammalian expression vector pcDNA3.1(+) or into the insect cell expression vector pVL1392. Expressed in H5 and Sf9 insect cells. Mutagenesis PCR-products transformed into Escherichia coli TOP 10
active GzmH and mutant S182A with a C-terminal 6 x His-tag coding sequence subcloned into vector pPICZalpha and expressed in Pichia pastoris. Jurkat or HeLa cells loaded with GzmH
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cDNA subcloned from pPIC-9-GrzmB yeast-expression vector into pcDNA3.1(+)
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cloned and expressed in Pichia pastoris
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commercial preparation
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CTLL R8 cell line stably transfected with GFP reporter constructs
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ectopic expression of granzyme B-epidermal growth factor receptor peptide ligand transforming growth factor alpha chimeric enzyme GrB-TGFalpha in natural killer cells from a lentiviral vector, the fusion enzyme shows enhanced natural cytotoxicity and increased specific killing of tumor cells
expressed in bacteria and eukaryotic cells. The large scale production of recombinant GzmB in bacteria may necessitate refolding, but this is easily achieved in optimized detergent-free refolding buffers
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expressed in Pichia pastoris
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expression in HEK-293 cells
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expression in Pichia pastoris
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expression of active GrB in yeast and by baculovirus expression
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expression of recombinant GrB using a baculovirus expression system
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expression of recombinant human granzyme B in COS cells
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functional recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
granzyme B cloning from the chymase locus, sequence comparisons and phylogenetic analysis, recombinant expression of inactive enzyme with N-terminal His6-tag followed by an enterokinase (EK) site in HEK 293 EBNA cells
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into vector pPIC6alpha and expressed in Pichia pastoris X-33 cells
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leukocyte cDNA amplified by PCR, expressed in a baculovirus system in Sf9 cells
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recombinant expression of functional and nonfunctional forms of bovine granzyme B in COS-7 cells. Establishment of an enzymatic assay to detect and quantify the expression of functional granzyme B protein. Using this assay, the levels of killing of different Theileria parva-specific CD8+ T-cell clones are found to be significantly correlated with the levels of granzyme B protein but not the levels of mRNA transcript expression
recombinant expression of functional fluorescent chimeric GZMB-Tom mutant enzyme fusion protein in murine CD8+ T-cells
recombinant expression of His-tagged inactive enzyme in HEK-293 cells
recombinant granzyme B expressed from baculovirus in Sf9 cells
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recombinant granzyme B expressed in Pichia pastoris as a chimeric zymogen comprising the alpha-factor signal sequence, a prodomain including an enterokinase cleavage site, and the mature granzyme B sequence followed by a hexahistidine tag, cloned and expressed in Escherichia coli, resulting fusion protein is insoluble and folded incorrectly
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recombinant human ProGrB is produced in Pichia pastoris
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recombinant, nonglycosylated GzmH and GzmH mutant expressed in Escherichia coli
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recombinantly expressed
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sequence comparisons and phylogenetic analysis, recombinant expression of inactive enzyme with N-terminal His6-tag followed by an enterokinase (EK) site in HEK 293 EBNA cells