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3.4.21.75: Furin

This is an abbreviated version!
For detailed information about Furin, go to the full flat file.

Word Map on EC 3.4.21.75

Reaction

Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-/- bonds, where Xaa can by any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors =

Synonyms

Bfurin, Dfurin1, Dibasic processing enzyme, FUR, furin, furin A, furin B, furin convertase, furin protease, furin-like pro-protein convertase, furin-like prohormone convertase, furin-like proprotein convertase, furin-like protease, furin-like proteinase, FurinA, furinase, hfurin, KPC-1, More, PACE, Paired basic amino acid cleaving enzyme, Paired basic amino acid converting enzyme, Paired basic amino acid residue cleaving enzyme, PC1, PC1/3, PC2, PCSK3, proconvertase, prohormone convertase, prohormone convertase 1, prohormone convertase 2, Proprotein convertase, proprotein convertase subtilisin/kexin 3, proprotein convertase subtilisin/kexin type 3, proprotein convertase subtilisin/kexin type3, Serine proteinase PACE, Sfurin, sheddase, SPC3, subtilisin-like proprotein convertase, subtilisin-like protein convertase, Trans golgi network protease furin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.75 Furin

Purification

Purification on EC 3.4.21.75 - Furin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by metal-chelating chromatography
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by Ni2+ affinity chromatography
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on Ni2+-NTA column, 64fold
partial
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partially, recombinant hfurin
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purification protocol combining the heterologous expression of furin from CHO cells with an affinity step that efficiently extracts only active furin from the conditioned medium by using furin-specific inhibitor moieties as bait. The best affinity tag used, biotin-(8-(amino)-3,6-dioxa-octanoyl)2-Arg-Pro-Arg-4-4-amidinobenzylamide coupled to streptavidin-Sepharose beads, is used in a three-step chromatographic protocol and routinely results in a high yield of a homogeneous furin preparation with a specific activity of about 60 units/mg protein
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recombinant enzyme 300fold from HEK-293 cells by metal affinity chromatography, inhibitor based affinity chromatography, and gel filtration
recombinant enzyme mutant R466G/K468G from HEK-293T cells
recombinant FLAG/His6-tagged furin ectodomain from CHO cells or Spodoptera frugiperda Sf9 cells by nickel affinity chromatography, ultrafiltration, and gel filtration, to homogeneity
recombinant furin
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recombinant furin from the stably transfected Sf9 insect cells
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recombinant glycosylated His-tagged furin from HEK-293S cells by nickel affinity and inhibitor affinity chromatography, followed by gel filtration
recombinant Her2-antigen e23sFv-TD-tBID from Escherichia coli strain M15
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recombinant His6-tagged enzyme from Nicotiana benthamiana leaves by nickel affinity chromatography, co-purification of the coexpressed proteins
to homogeneity
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