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3.4.21.74: venombin A

This is an abbreviated version!
For detailed information about venombin A, go to the full flat file.

Word Map on EC 3.4.21.74

Reaction

Selective cleavage of Arg-/- bond in fibrinogen, to form fibrin, and release fibrinopeptide A. The specificity of further degradation of fibrinogen varies with species origin of the enzyme =

Synonyms

AaV-SP-II, albofibrase1, alpha fibrinogenase, alpha-Fibrinogenase, Ancrod, Batroxobin, beta-fibrinogenase, Bhalternin, bothrombin, Bothrops atrox serine proteinase, BpirSP-39, coagulant serine protease, Crotalase, Dav-PA, EC 3.4.21.28, EC 3.4.21.29, EC 3.4.21.30, gloshedobin, Habutobin, Lachesis stenophrys venom serine proteinase, metalloproteinase 1, Mutase, acetolactate, Protein C activator, Reptilase, Russell's viper basic coagulant metalloprotease, RVBCMP, RVV protease, snake venom serine protease, TA-2, Thrombin-like enzyme, thrombin-like serine protease, TLE, Vaa serine proteinase homolog 1, VaaSPH-1, VaF1, venom gloshedobin, viper venom serine protease, VLAF, VLBF, VLCII

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.74 venombin A

Cloned

Cloned on EC 3.4.21.74 - venombin A

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Bothrops atrox, moojeni
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cDNA cloning
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cDNA encoding ancrod synthesized with a yeast bias codon and inserted into the eukaryotic expression vector pPIC9, subsequently expressed in Pichia pastoris strain GS115
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DNA and amino acid sequence determination and analysis
expression of the enzyme N-terminally fused separately to three fusion partners, NusA, GST, and TrxA, expression in Escherichia coli strain BL21-Gold (DE3). NusA is the most efficient fusion partner to improve the solubility of recombinant gloshedobin, subcloning in Escherichia coli strain JM109
pET-r-habutobin expressed in inclusion body of Escherichia coli. Autographa californica nuclear polyhedrosis virus and the habutobin/pPSC12 plasmid co-transfected into Sf9 cells
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recombinant albofibrase1 expressed in Pichia pastoris strain X-33
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