Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.21.73: u-Plasminogen activator

This is an abbreviated version!
For detailed information about u-Plasminogen activator, go to the full flat file.

Word Map on EC 3.4.21.73

Reaction

Specific cleavage of Arg-/-Val bond in plasminogen to form plasmin =

Synonyms

Abbokinase, Cellular plasminogen activator, Double-chain urokinase-type plasminogen activator, EC 3.4.21.31, EC 3.4.99.26, Plasminogen activator, urokinase-type, PLAU, Two-chain urokinase-type plasminogen activator, u-PA, U-plasminogen activator, UK, uPA, uPA-type Plg activator, Urinary esterase A, Urinary plasminogen activator, Urokinase, Urokinase plasminogen activator, urokinase type plasminogen activator, urokinase-plasminogen activator, urokinase-type PA, urokinase-type plasmin activator, urokinase-type plasminogen, Urokinase-type plasminogen activator, urokinase-type Plg activator, urokine-type plasminogen activator

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.73 u-Plasminogen activator

Cloned

Cloned on EC 3.4.21.73 - u-Plasminogen activator

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amino-terminal fragment of human uPA cloned and expressed in Saccharomyces cerevisiae
-
cDNA cloning
-
cloning of low molecular weight uPA, expression in Pichia pastoris
-
DNA and amino acid sequence analysis
-
expression in Escherichia coli
-
expression in S2 cells
-
expression of mutant enzymes in HEK-293T cells
-
expression of mutant S195A in Pichia pastoris strain X-33
expression of the enzyme's kringle domain UK1 in Pichia pastoris
-
expression of wild-type and mutant enzymes in HEK-293T cells
-
expression using vesicular stomatitis virus G pseudo-typed lentiviruses
-
functional overexpression of the enzyme in hepatic stellate cells, using an adenoviral vector system
-
macrophage-specific uPA overexpression accelerates atherosclerosis
-
mutagenesis throughout the single-chain urokinase-type plasminogen activator
-
mutant C122S expressed in Escherichia coli inclusion bodies, B-chain uPA expressed in S. pastoris
-
mutation affecting the activity
-
overexpression of urokinase plasminogen activator in macrophages, elevation of the pro-inflammatory cytokine interleukin-6 in hearts of transgenic mice, interleukin-6 is not a major effector of the enzyme-induced cardiac fibrosis, transcriptome analysis, overview
-
quantitative uPA expression analysis
-
recombinant expression of mutant enzyme
-
recombinant expression of wild-type enzyme
-
semiquantitative reverse transcription PCR expression analysis of uPA and uPAR, overview
-
uPA quantitative real-time RT-PCR expression analysis in oral squamous cell carcinoma, overview
-