Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.21.68: t-Plasminogen activator

This is an abbreviated version!
For detailed information about t-Plasminogen activator, go to the full flat file.

Word Map on EC 3.4.21.68

Reaction

Specific cleavage of Arg-/-Val bond in plasminogen to form plasmin =

Synonyms

Alteplase, BAT-PA, desmoteplase, DSPA beta, DSPA gamma, EC 3.4.21.31, EC 3.4.99.26, human tissue-type plasminogen activator, IV t-PA, IV tissue plasminogen activator, More, plasminogen activator, Plasminogen activator, tissue-type, PLAT, r-tPA, recombinant tissue plasminogen activator, Reteplase, rt-PA, rtPA, sc-tPA, sctPA, single chain enzyme tPA, t-PA, t-PA protein, t-plasminogen activator, tc-tPA, tctPA, Tissue plasminogen activator, tissue type plasminogen activator, tissue-plasminogen activator, Tissue-type plasminogen activator, tissue-type plasmiongen activator, tPA, two chain tPA

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.68 t-Plasminogen activator

KM Value

KM Value on EC 3.4.21.68 - t-Plasminogen activator

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004 - 0.39
(p-F)-FPRANSNH-C2H5
0.48
acetyl-FTAR-4-nitroanilide
pH and temperature not specified in the publication
0.59
acetyl-FYAR-4-nitroanilide
pH and temperature not specified in the publication
1.1
acetyl-GTAR-4-nitroanilide
pH and temperature not specified in the publication
0.76
acetyl-GTGR-4-nitroanilide
pH and temperature not specified in the publication
1.3
acetyl-QYAR-4-nitroanilide
pH and temperature not specified in the publication
0.86
Asp-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
2.3
benzyloxycarbonyl-Asp(O-tert-butyl)-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.72
benzyloxycarbonyl-Asp-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.52
benzyloxycarbonyl-D-Arg-Gly-Arg-4-nitroanilide
-
pH 8.0, 25°C
1.8
benzyloxycarbonyl-D-Asp(O-tert-butyl)-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
1.2
benzyloxycarbonyl-D-Glu(O-tetr-butyl)-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
2.6
benzyloxycarbonyl-D-Glu-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.3
benzyloxycarbonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.9
benzyloxycarbonyl-Glu-(O-tert-butyl)Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
2.2
benzyloxycarbonyl-Glu-Gly-Arg-4-nitroanilide
-
pH 8.0, 25°C
-
1.36
benzyloxycarbonyl-Val-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.0004 - 0.071
Boc-(p-F)-FPRANSNH-C2H5
0.013 - 0.03
Boc-D-FLRANSNH-C3H7
0.0008
Boc-D-FLRANSNHC3H7
-
pH 7.4, 25°C, ratio tc/sc
0.0026 - 0.047
Boc-D-FPRANSNH-C6H11
0.0027 - 0.091
Boc-D-FVRANSNH-C2H5
0.0004 - 0.023
Boc-D-LGRANSNH-C6H11
0.0006 - 0.066
Boc-D-LPRANSNH-C3H7
0.0006 - 0.36
Boc-D-LSRANSNH-C3H7
0.0007 - 0.14
Boc-D-VGRANSNH-C4H9
0.0005
Boc-D-VPR-ANSNHC4H9
-
pH 7.4, 25°C, ratio tc/sc
0.015 - 0.029
Boc-D-VPRANSNH-C4H9
0.1
Boc-L-VGRANSNHC4H9
-
pH 7.4, 25°C
5.32
cyclohexylglycyl-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
1.7
D-Asp(O-tert-butyl)-Gly-Arg-4-nitroanilide
-
pH 8.0, 25°C
-
1.53
D-cyclohexylglycyl-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.0074 - 0.01
D-FLRANSNH-C3H7
0.00078
D-FPR-ANSNH-C2H4OCH3
-
pH 7.4, 25°C, ratio tc/sc
0.00046
D-FPR-ANSNH-CH2COOCH3
-
pH 7.4, 25°C, ratio tc/sc
0.099
D-FPR-ANSNH-n-C6H13
-
pH 7.4, 25°C, sc-tPA
0.00038 - 0.25
D-FPRANSN-(CH2)6
0.00037 - 0.32
D-FPRANSN-C2H5-C2H5
0.00059 - 0.15
D-FPRANSNH-c-C6H11
0.14 - 0.18
D-FPRANSNH-C2H4OCH3
0.0057 - 0.077
D-FPRANSNH-C6H11
0.0004 - 0.063
D-FPRANSNH-C6H5CH2
0.029 - 0.063
D-FPRANSNH-CH2COOCH3
0.00022 - 0.33
D-FPRANSNH-i-C3H7
0.0004 - 0.12
D-FPRANSNH-n-C4H9
0.095 - 0.096
D-FPRANSNH-n-C6H13
0.0011 - 0.084
D-FPRANSNH-t-C4H9
0.0011 - 0.016
D-FVRANSNH-C2H5
2.9
D-Glu-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.83
D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.000086 - 0.694
D-Ile-Pro-Arg-p-nitroanilide
0.001 - 0.041
D-LGRANSNH-C6H11
0.0003 - 0.098
D-LPRANSNH-C3H7
0.0012 - 0.069
D-LSRANSNH-C3H7
2.1
D-Lys-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.7
D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.0015 - 0.058
D-VGRANSNH-C4H9
0.0003 - 0.11
D-VPRANSNH-C4H9
3.2
Glu-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.00002 - 0.065
Glu-plasminogen
-
0.036 - 0.97
L-FPRANSNH-C2H5
0.46
L-VGRANSNH-C4H9
-
pH 7.4, 25°C
5.9
Lys-Lys-Cys-Pro-Gly-Arg-Val-Val-Gly-Gly-Cys-Val-Ala-His
-
-
3.6
Lys-Lys-Ser-Pro-Gly-Arg-Val-Val-Gly-Gly-Ser-Val-Ala-His
-
-
0.00001 - 0.019
Lys-plasminogen
-
0.34
methyloxycarbonyl-D-cyclohexylalanyl-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.42
methyloxycarbonyl-D-cyclohexylglycyl-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.39
methyloxycarbonyl-D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.62
methyloxycarbonyl-D-Leu-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.99
methyloxycarbonyl-D-Nle-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.8
methyloxycarbonyl-D-Val-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.1
methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.000017
Peptide S-2288
-
-
3.15
pGlu-Gly-Arg-4-nitroanilide
-
pH 8.0, 25°C
-
2
Phe-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.000018 - 0.065
plasminogen
-
3.5 - 4.1
S-2288TM
-
0.4 - 4.6
spectrozyme tPA
3.5
tert-butoxycarbonyl-Gly-Gly-Arg-p-nitroanilide
-
pH 8.0, 25°C
0.14
[SNase]-PFGRSA
-
pH 7.4, 37°C
-
0.079
[SNase]-PFGRSAG
-
pH 7.4, 37°C
-
0.0099
[SNase]-PGPFGRSAG
-
pH 7.4, 37°C
-
0.0093
[SNase]-PGPFGRSAGG
-
pH 7.4, 37°C
-
0.017
[SNase]-PGSGRSAG
-
pH 7.4, 37°C
-
0.018
[SNase]-PHYGRSGG
-
pH 7.4, 37°C
-
0.0099
[SNase]-PPFGRSAG
-
pH 7.4, 37°C
-
0.0089
[SNase]-PQRGRSAG
-
pH 7.4, 37°C
-
additional information
additional information
-