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0.022
acetyl-L-Phe
-
30°C, pH 8.0
0.38
acetyl-L-Phe ethyl ester
-
30°C, pH 8.0
0.1
acetyl-L-Tyr
-
30°C, pH 8.0
0.04
acetyl-L-Tyr ethyl ester
-
30°C, pH 8.0
1900 - 2500
benzyloxycarbonyl-Gly-4-nitroanilide
-
16550
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide
-
-
5.186
casein
recombinant enzyme, pH 8.0, 60°C
64.3 - 80
Glp-Ala-Ala-Leu-p-nitroanilide
35.5
Hammarsten casein
-
37°C, pH 10.5
-
31 - 47
L-Ala-L-Ala-L-Pro-L-Ala
185 - 403
L-Ala-L-Ala-L-Pro-L-Phe
10 - 12
L-Ala-L-Ala-L-Val-L-Ala
90 - 189
L-Phe-L-Ala-L-Ala-L-Phe
4 - 10
L-Tyr-L-Val-L-Ala-L-Asp
47 - 97
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
0.0064 - 0.0089
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
231.3 - 586.2
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide
1.7 - 759
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
1.6 - 440
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
70.2
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
-
37°C, pH 10.5
222 - 97000
Suc-Ala-Ala-Ala-p-nitroanilide
93 - 3700
Suc-Ala-Ala-p-nitroanilide
222 - 130000
Suc-Ala-Ala-Pro-Ala-p-nitroanilide
389 - 97000
Suc-Ala-Ala-Pro-Leu-p-nitroanilide
90 - 200
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
0.052 - 28.2
succinyl-AAPA-p-nitroanilide
1.75 - 14.5
succinyl-AAPE-p-nitroanilide
0.017 - 153
succinyl-AAPF-p-nitroanilide
0.16 - 6.08
succinyl-AAPR-p-nitroanilide
1.6 - 1.8
Succinyl-Ala-Ala-Ala 4-nitroanilide
52
succinyl-Ala-Ala-Phe-4-nitroanilide
-
subtilisin DY
45 - 57
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
148 - 130000
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
6.4
succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin
-
mutant Y217L, pH 8.3, 25°C
15 - 141
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
210
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide
-
mutant Y217L, pH 8.3, 25°C
68
succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin
-
mutant Y217L, pH 8.3, 25°C
29
succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl 7-amide
-
subtilisin Sendai
0.04
Urea-denatured hemoglobin
-
30°C, pH 8.0
-
70.9 - 104
Z-Ala-Ala-Leu-p-nitroanilide
additional information
additional information
-
1900
benzyloxycarbonyl-Gly-4-nitroanilide
-
pH 7.5, 20°C
-
2500
benzyloxycarbonyl-Gly-4-nitroanilide
-
pH 7.5, 20°C, DTT
-
64.3
Glp-Ala-Ala-Leu-p-nitroanilide
-
in 2% DMF
69
Glp-Ala-Ala-Leu-p-nitroanilide
-
in 2% DMF
80
Glp-Ala-Ala-Leu-p-nitroanilide
-
in 2% DMSO
31
L-Ala-L-Ala-L-Pro-L-Ala
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
47
L-Ala-L-Ala-L-Pro-L-Ala
-
wild-type, pH 9.0, 24°C
185
L-Ala-L-Ala-L-Pro-L-Phe
-
wild-type, pH 9.0, 24°C
403
L-Ala-L-Ala-L-Pro-L-Phe
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
10
L-Ala-L-Ala-L-Val-L-Ala
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
12
L-Ala-L-Ala-L-Val-L-Ala
-
wild-type, pH 9.0, 24°C
90
L-Phe-L-Ala-L-Ala-L-Phe
-
wild-type, pH 9.0, 24°C
189
L-Phe-L-Ala-L-Ala-L-Phe
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
4
L-Tyr-L-Val-L-Ala-L-Asp
-
V104S/L124M/P129S/S130G/P131E/A133S/T134L/L135I, pH 9.0, 24°C
10
L-Tyr-L-Val-L-Ala-L-Asp
-
wild-type, pH 9.0, 24°C
47
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
subtilisin DY
57
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
subtilisin Carlsberg
57
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
subtilisin Carlsberg
97
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide
-
subtilisin
0.0064
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C
0.0074
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C, mutant D180G
0.0079
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C, mutant D182G
0.0089
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C
0.0089
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide
-
pH 8.5, 37°C, mutant D180G/D182G
231.3
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.4, 37°C, wild-type
586.2
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.4, 37°C, mutant S101W/G169A/V192A
1.7
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant mutant T58A/G165I/L216W, pH 7.5, 30°C
15.1
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant mutant T58A/G165L/L216W, pH 7.5, 30°C
22.8
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant mutant T58A/G165Y/L216W, pH 7.5, 30°C
33.1
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant mutant T58A/L216W, pH 7.5, 30°C
40.8
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 5°C, pH 8.6
172.6
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant wild-type enzyme, pH 7.5, 30°C
193.1
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 25°C, pH 7.4
225.7
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 25°C, pH 8.6
248.7
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 25°C, pH 9.4
419
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant wild-type enzyme
462
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant wmutant T211G
475
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant N212G
552.3
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
recombinant enzyme, 45°C, pH 8.6
638
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant P210G
697
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant P210G/T211G
715
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant P210A
759
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
pH 8.0, 25°C, recombinant mutant P210G/T211G/N212G
1.6
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
subtilisin-like serine protease, at 20°C, in 50 mM Tris-HCl buffer, pH 7.5
14
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
subtilisin, at 20°C, in 50 mM Tris-HCl buffer, pH 8.0
25
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
subtilisin-like serine protease, at 80°C, in 50 mM Tris-HCl buffer, pH 7.5
440
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
-
subtilisin, at 80°C, in 50 mM Tris-HCl buffer, pH 8.0
222
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 5°C
750
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 20°C, DTT
1200
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 20°C
22000
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 40°C
97000
Suc-Ala-Ala-Ala-p-nitroanilide
-
pH 7.5, 75°C
93
Suc-Ala-Ala-p-nitroanilide
-
pH 7.5, 20°C, in presence and absence of DTT
3700
Suc-Ala-Ala-p-nitroanilide
-
pH 7.5, 40°C
222
Suc-Ala-Ala-Pro-Ala-p-nitroanilide
-
pH 7.5, 5°C
8800
Suc-Ala-Ala-Pro-Ala-p-nitroanilide
-
pH 7.5, 40°C
130000
Suc-Ala-Ala-Pro-Ala-p-nitroanilide
-
pH 7.5, 75°C
389
Suc-Ala-Ala-Pro-Leu-p-nitroanilide
-
pH 7.5, 5°C
97000
Suc-Ala-Ala-Pro-Leu-p-nitroanilide
-
pH 7.5, 40°C
90
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
3.5 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C
132
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
2.2 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C
137
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
recombinant enzyme, pH 8.5, 22°C
166
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
1 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C
200
Suc-Ala-Ala-Pro-Phe-4-nitroanilide
-
subtilisin without bound lactose, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C
0.052 - 2.1
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-g
6.8
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-c and S166C-S-e
9.65
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-g
17.7
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C wild-type
28.2
succinyl-AAPA-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-f
1.75
succinyl-AAPE-p-nitroanilide
-
pH 8.6, 25°C wild-type
14.5
succinyl-AAPE-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-a
0.017
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-a
0.49
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-c
0.833
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-f
1.04
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-f
1.64
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-e
2.94
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-e
3.7
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-e
3.8
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-e
4.8
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-f
6.08
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-f
6.9
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-d and M222C-S-d
7.2
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-b
11.8
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-b
14.2
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C
16.3
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-f
16.9
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-c
17.3
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C
17.4
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-a
18
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-c
21.8
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-d
23.1
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-c
23.4
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-b
24.2
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-c
25
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-b
29
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-g
30
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-a
31
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S166C-S-c
31.6
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-e
33.3
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant M222C-S-a
39
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C-S-f
43
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C mutant S156C
47
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-d
48
succinyl-AAPF-p-nitroanilide
-
pH 7.5, 25°C, wild-type
48
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C, mutant S166C-h
50
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-a
67
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-i
76
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-j
82
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-e
153
succinyl-AAPF-p-nitroanilide
-
pH 8.6, 25°C wild-type
0.16
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C wild-type
0.17
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-b
0.35
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-h
0.43
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-i
0.68
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-d
1.06
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-j
6.08
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-d
6.08
succinyl-AAPR-p-nitroanilide
-
pH 8.6, 25°C mutant S166C-S-j
1.6
Succinyl-Ala-Ala-Ala 4-nitroanilide
-
subtilisin Carlsberg
1.8
Succinyl-Ala-Ala-Ala 4-nitroanilide
-
subtilisin DY
45
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
mutant subtilisin BPN' Asp99Ser
57
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
subtilisin BPN'
57
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide
-
subtilisin BPN'
148
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 5°C
2900
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 20°C, DTT
10000
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 20°C
130000
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide
-
pH 7.5, 40°C
15
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant wild-type enzyme, in presence of 2 M GdmCl
34
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V, in presence of 2 M GdmCl
35
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant wild-type enzyme, in presence of SDS
42
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V, in absence of GdmCl or SDS
45
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V, in presence of SDS
50
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant wild-type enzyme, in absence of GdmCl or SDS
62
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant G166M, in presence of 2 M GdmCl
65
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant S62I, in presence of SDS
70
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant S62I/G166M, in presence of 2 M GdmCl
81
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant S62I/G166M, in presence of SDS
102
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant S62I, in absence of GdmCl or SDS
104
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant G166M, in presence of SDS
123
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant S62I/G166M, in absence of GdmCl or SDS
141
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide
pH 8.5, 37°C, recombinant mutant G166M, in absence of GdmCl or SDS
70.9
Z-Ala-Ala-Leu-p-nitroanilide
-
in 20% DMF
77
Z-Ala-Ala-Leu-p-nitroanilide
-
in 20% DMSO
100
Z-Ala-Ala-Leu-p-nitroanilide
-
in 20% DMSO
104
Z-Ala-Ala-Leu-p-nitroanilide
-
in 20% DMF
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
turnover number of subtilisin Novo chemically attached to soluble DEAE-dextran and insoluble DEAE-Sephadex
-
additional information
additional information
-
increasing the level of glycosylation causes a linearly dependent reduction in structural dynamics, which leads to a decrease in the catalytic turnover rate for both, the enzyme acylation and deacylation steps
-