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3.4.21.62: Subtilisin

This is an abbreviated version!
For detailed information about Subtilisin, go to the full flat file.

Word Map on EC 3.4.21.62

Reaction

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides =

Synonyms

AcpII, Ak.1 protease, Alcalase, Alcalase 0.6L, Alcalase 2.5L, ALE1 subtilase, ALK-enzyme, Alkaline mesentericopeptidase, Alkaline protease, alkaline serine protease, ALP1, Alzwiprase, aprE, AprE51, aqualysin, Arp, AsES, Asp v 13, AtSBT1.9, Bacillopeptidase A, Bacillopeptidase B, Bacillus gibsonii alkaline protease, Bacillus subtilis alkaline proteinase Bioprase, BgAP, Bioprase AL 15, Bioprase APL 30, BLS, BPN', BprB, BprV, C1 subtilase, cold active subtilisin-like serine proteinase, Colistinase, EC 3.4.21.14, EC 3.4.4.16, Esperase, Fe protease, Fe prtS8A, Genenase I, intracellular subtilisin protease, ISP, IvaP, Kazusase, Maxatase, mesenteroicopeptidase, More, Nagarse, Opticlean, ORF2, Orientase 10B, P69 subtilase, PBANKA_1106900, PbSOPT, Peptidase, subtilo-, A, PF3D7_0507300, phytophase, PIMMS2, Protease S, Protease VIII, Protease XXVII, Proteinase K, Proteinase, Bacillus subtilis alkaline, Protin A 3L, PSP-3, psychrophilic subtilisin-like protease, S1P subtilase, SAP, SAS-1, SASP, saspase, Savinase, Savinase 16.0L, Savinase 32.0 L EX, Savinase 4.0T, Savinase 8.0L, savinaseTM, SBc, SBL, SDD1 subtilase, senescence-associated subtilisin protease, SES7, SISBT3 subtilase, SOPT, SP 266, Sspa, SSU0757, SUB1, SUB2, subC, subtilase, subtilase subfamily 1 member 9, subtilase-like protease, subtilisin, subtilisin 72, subtilisin A, Subtilisin amylosacchariticus, Subtilisin BL, subtilisin BPN’, subtilisin C., subtilisin Carlsberg, subtilisin DJ-4, Subtilisin DY, Subtilisin E, subtilisin E-S7, Subtilisin GX, subtilisin JB1, subtilisin Karlsberg, Subtilisin Novo, subtilisin Pr1-like protease, subtilisin protease, subtilisin QK, Subtilisin S41, subtilisin S4I, subtilisin S88, Subtilisin Sendai, subtilisin Sph, subtilisin-like ookinete protein, subtilisin-like ookinete protein important for transmission, subtilisin-like protease, subtilisin-like protease AprV2, subtilisin-like serine protease, Subtilisn J, Subtilopeptidase, Superase, thermitase, thermo-active subtilisin-like serine protease, Thermoase, Thermoase PC 10, thermophilic thermitase, thermostable subtilisin, ThSS45, Tk-subtilisin, trans-cinnamoyl-subtilisin, V. cholerae-secreted serine protease, VC_0157, vPR

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.62 Subtilisin

Substrates Products

Substrates Products on EC 3.4.21.62 - Subtilisin

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R,S)-2-benzyl-3-(tert-butylsulfonyl)propionic acid + H2O
(R)-2-benzyl-3-(tert-butylsulfonyl)propionic ethyl ester + (S)-2-benzyl-3-(tert-butylsulfonyl)propionic acid + ethanol
show the reaction diagram
-
-
-
-
?
(R,S)-2-benzyl-3-[[1-methyl-1-((morpholin-4-yl)-carbonyl)ethyl]sulfonyl]propionic acid + H2O
(S)-2-benzyl-3-[[1-methyl-1-((morpholin-4-yl)-carbonyl)ethyl]sulfonyl]propionic acid + (R)-2-benzyl-3-[[1-methyl-1-((morpholin-4-yl)-carbonyl)ethyl]sulfonyl]propionic acid-ethyl ester + ethanol
show the reaction diagram
-
-
-
-
?
(R/S)-(2-methylpropyl)butanedioic acid diethyl ester + H2O
(S)-(2-methylpropyl)butanedioic acid diethyl ester + (R)-(2-methylpropyl)butanedioic acid 4-ethyl ester + ethanol
show the reaction diagram
-
-
-
-
?
1-phenylethanol + trifluoroethyl butyrate
?
show the reaction diagram
-
-
-
-
?
1-propanol + N-acetyl phenylalanine ethyl ester
?
show the reaction diagram
-
-
-
-
?
2 rac-1-(4-chlorophenyl)ethan-1-ol + vinyl butyrate
(1S)-1-(4-chlorophenyl)ethyl butyrate + (1R)-1-(4-chlorophenyl)ethan-1-ol + ?
show the reaction diagram
-
-
-
?
2 rac-1-(4-methoxyphenyl)ethan-1-ol + vinyl butyrate
(1S)-1-(4-methoxyphenyl)ethyl butyrate + (1R)-1-(4-methoxyphenyl)ethan-1-ol + ?
show the reaction diagram
substrate only of mutant G165L/M221F, not of the wild-type enzyme
-
-
?
2 rac-1-(4-methylphenyl)ethan-1-ol + vinyl butyrate
(1S)-1-(4-methylphenyl)ethyl butyrate + (1R)-1-(4-methylphenyl)ethan-1-ol + ?
show the reaction diagram
substrate only of mutant G165L/M221F, not of the wild-type enzyme
-
-
?
2 rac-1-phenylbutan-1-ol + vinyl butyrate
(S)-1-phenylbutyl butyrate + (R)-1-phenylbutan-1-ol + ?
show the reaction diagram
substrate only of mutant G165L/M221F, not of the wild-type enzyme
-
-
?
2 rac-1-phenylethanol + vinyl butyrate
(S)-1-phenylethyl butyrate + (R)-1-phenylethanol + ?
show the reaction diagram
-
-
-
?
2 rac-1-phenylheptan-1-ol + vinyl butyrate
(S)-1-phenylheptyl butyrate + (R)-1-phenylheptan-1-ol + ?
show the reaction diagram
substrate only of mutant G165L/M221F, not of the wild-type enzyme
-
-
?
2 rac-1-phenylpentan-1-ol + vinyl butyrate
(S)-1-phenylpentyl butyrate + (R)-1-phenylpentan-1-ol + ?
show the reaction diagram
substrate only of mutant G165L/M221F, not of the wild-type enzyme
-
-
?
2 rac-1-phenylpropan-1-ol + vinyl butyrate
(S)-1-phenylpropyl butyrate + (R)-1-phenylpropan-1-ol + ?
show the reaction diagram
substrate only of mutant G165L/M221F, not of the wild-type enzyme
-
-
?
2 rac-N-tert-butoxycarbonylphenylalanine ethyl thioester + 1-phenylmethanamine + tert-butanol
N-benzyl-Nalpha-(tert-butoxycarbonyl)-L-phenylalaninamide + (R)-N-tert-butoxycarbonylphenylalanine ethyl thioester + ?
show the reaction diagram
-
continuous-flow cascade reactor system for subtilisin A-catalyzed dynamic kinetic resolution (DKR). The continuous-mode DKR of the racemic thioester in a serial cascade system of six biocatalyst-filled columns at 50°C for KR and five grafted silica gel-filled columns at 150°C for racemization results in the formation of the (S)-benzylamide in 79% conversion, 8.17 g/l/h volumetric productivity and 98% enantiomeric excess. Method evaluation, overview
-
-
?
67 kDa gamma-glutamyl transpeptidase + H2O
30 kDa gamma-glutamyl transpeptidase + ?
show the reaction diagram
-
proteolytic digestion of 67 kDa gamma-glutamyl transpeptidase from Bacillus licheniformis ER-15 by subtilisin to the 30 kDa form, which in turn remains associated with subtilisin as a heterodimeric protein
-
-
?
Aalpha chain of fibrinogen + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-L-Ala-L-Ala-L-Ala-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
acetyl-L-Phe + ethanol
acetyl-L-Phe ethyl ester + H2O
show the reaction diagram
acetyl-L-Phe ethyl ester + H2O
acetyl-L-Phe + ethanol
show the reaction diagram
acetyl-L-Tyr + ethanol
acetyl-L-Tyr ethyl ester + H2O
show the reaction diagram
-
-
-
-
r
acetyl-L-Tyr ethyl ester + H2O
acetyl-L-Tyr + ethanol
show the reaction diagram
acid casein + H2O
?
show the reaction diagram
-
purified caseins from animal's milk from cow, sheep, goat and water buffalo used as substrate for subtilisin, cow acid casein is the best substrate
-
?
Ala-Ala-Phe 7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Ala-Ala-Pro-Leu-4-nitroanilide + H2O
Ala-Ala-Pro-Leu + 4-nitroaniline
show the reaction diagram
low activity
-
-
?
Ala-Ala-Pro-Lys-4-nitroanilide + H2O
Ala-Ala-Pro-Lys + 4-nitroaniline
show the reaction diagram
low activity
-
-
?
Ala-Ala-Pro-Met-4-nitroanilide + H2O
Ala-Ala-Pro-Met + 4-nitroaniline
show the reaction diagram
low activity
-
-
?
Ala-Ala-Pro-Nle-4-nitroanilide + H2O
Ala-Ala-Pro-Nle + 4-nitroaniline
show the reaction diagram
low activity
-
-
?
Ala-Ala-Pro-Phe 4-nitroanilide + H2O
?
show the reaction diagram
Ala-Ala-Pro-Phe-4-nitroanilide + H2O
Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
low activity
-
-
?
Ala-Ala-Val-Ala-4-nitroanilide + H2O
Ala-Ala-Val-Ala + 4-nitroaniline
show the reaction diagram
low activity
-
-
?
alpha-casein + H2O
?
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
Benzoyl-Arg ethyl ester + H2O
?
show the reaction diagram
benzoyl-L-Arg + ethanol
benzoyl-L-Arg ethyl ester + H2O
show the reaction diagram
-
-
-
-
r
Benzoyl-L-Arg ethyl ester + H2O
Benzoyl-L-Arg + ethanol
show the reaction diagram
Benzyloxycarbonyl-Ala-Ala-Leu 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-4-nitroanilide + H2O
benzyloxycarbonyl-Gly + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Gly-Gly-L-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Gly-L-Leu-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Gly-L-Tyr-NH2 + H2O
?
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-glycyl-L-tyrosinamide + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-Ala ethyl ester + butanol
benzyloxycarbonyl-L-Ala butyl ester + ethanol
show the reaction diagram
-
in isooctane, preferential use of L-enantiomer. Comparison with enantioselectivity in water and DMSO
-
-
?
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide + H2O
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu + 4-nitroaniline
show the reaction diagram
benzyloxycarbonyl-L-Asp methyl ester + serine amide
benzyloxycarbonyl-L-Asp-L-Ser-NH2 + methanol
show the reaction diagram
-
-
-
-
?
beta subunit of haemoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
beta-casein + H2O
?
show the reaction diagram
Bovine serum albumin + H2O
?
show the reaction diagram
Carboxybenzoyl-Gly-Ala-NH2 + H2O
Carboxybenzoyl-Gly-Ala + NH3
show the reaction diagram
-
subtilisin BPN'
subtilisin BPN'
?
Carboxybenzoyl-Gly-Leu-NH2 + H2O
Carboxybenzoyl-Gly-Leu + NH3
show the reaction diagram
-
subtilisin BPN'
subtilisin BPN'
?
Carboxybenzoyl-Gly-Pro-Leu-Gly-Pro-OH + H2O
Carboxybenzoyl-Gly-Pro-Leu + Gly-Pro-OH
show the reaction diagram
-
subtilisin BPN'
subtilisin BPN'
?
casein + H2O
?
show the reaction diagram
chicken-feather keratin + H2O
?
show the reaction diagram
-
-
-
-
?
chitin + H2O
?
show the reaction diagram
Clupein + H2O
?
show the reaction diagram
-
-
-
-
?
Collagen + H2O
?
show the reaction diagram
cuticle + H2O
?
show the reaction diagram
Cytochrome c + H2O
?
show the reaction diagram
D-Val-Leu-Lys p-nitroanilide + H2O
D-Val-Leu-Lys + p-nitroaniline
show the reaction diagram
-
-
-
?
eglin-c + H2O
?
show the reaction diagram
-
proteinase inhibitor, subtilisin DY hydrolyses the peptide bond between residues 45 and 46 in the reactive centre of eglin-c
-
?
Elastin + H2O
?
show the reaction diagram
ethyl 2-(4-ethylphenoxy)propionate + butanol
butyl 2-(4-ethylphenoxy)propanoate + propanol
show the reaction diagram
-
in isooctane, preferential use of S-enantiomer. Comparison with enantioselectivity in water and DMSO
-
-
?
Fibrin + H2O
?
show the reaction diagram
Fibrinogen + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
Glp-Ala-Ala-Leu-p-nitroanilide + H2O
?
show the reaction diagram
glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
glutaryl-Ala-Ala-Pro-Leu + p-nitroaniline
show the reaction diagram
relative hydrolysis rate is 22%
-
-
?
Hammarsten casein + H2O
?
show the reaction diagram
Hemoglobin + H2O
?
show the reaction diagram
Human fibrinogen + H2O
?
show the reaction diagram
-
fibrinolytic activity is determined using the fibrin plate method
-
-
?
human fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
insoluble elastin + H2O
?
show the reaction diagram
-
-
-
-
?
intelectin + H2O
?
show the reaction diagram
IvaP I9 domain + H2O
?
show the reaction diagram
a temporary inhibitor and substrate of purified IvaP
-
-
?
kappa casein + H2O
?
show the reaction diagram
-
purified caseins from animal's milk from cow, sheep, goat and water buffalo used as substrate for subtilisin
-
?
Keratin + H2O
?
show the reaction diagram
KOH chitin + H2O
?
show the reaction diagram
L-Ala-L-Ala-L-Ala-L-Ala-L-Pro-L-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
L-Ala-L-Ala-L-Phe-L-Ala-L-Ala-L-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
L-Ala-L-Ala-L-Pro-L-Ala + H2O
?
show the reaction diagram
-
-
-
-
?
L-Ala-L-Ala-L-Pro-L-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
L-Ala-L-Ala-L-Val-L-Ala + H2O
?
show the reaction diagram
-
-
-
-
?
L-Lys-L-His-L-Asp-L-Arg-L-Lys-L-Asp + H2O
?
show the reaction diagram
-
-
-
-
?
L-Phe-L-Ala-L-Ala-L-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
L-phenylalanine-isopropylester + H2O
L-phenylalanine + isopropanol
show the reaction diagram
-
-
-
-
?
L-Tyr-L-Val-L-Ala-L-Asp + H2O
?
show the reaction diagram
-
-
-
-
?
lactate dehydrogenase + H2O
?
show the reaction diagram
poor substrate in native form, but can be digested in heat-denatured form
-
-
?
malate dehydrogenase + H2O
?
show the reaction diagram
poor substrate in native form, but can be digested in heat-denatured form
-
-
?
MeO-succinyl-Ala-Ala-Phe 4-nitroanilide + H2O
?
show the reaction diagram
methoxysuccinyl-Ala-Ile-Pro-Met-p-nitroanilide + H2O
methoxysuccinyl-Ala-Ile-Pro-Met + p-nitroaniline
show the reaction diagram
is the most favorable substrate, relative hydrolysis rate is 100%
-
-
?
methyl mandelate + butanol
butyl mandelate + methanol
show the reaction diagram
-
in isooctane, preferential use of S-enantiomer. Comparison with enantioselectivity in water and DMSO
-
-
?
N-Acetyl-Ala methyl ester + H2O
?
show the reaction diagram
-
subtilisin BPN'
-
-
?
N-Acetyl-Gly ethyl ester + H2O
?
show the reaction diagram
-
subtilisin BPN'
-
-
?
N-Acetyl-L-norvaline ethyl ester + H2O
?
show the reaction diagram
-
subtilisin BPN'
-
-
?
N-acetyl-L-phenylalanine ethyl ester
?
show the reaction diagram
-
-
-
-
?
N-acetyl-L-phenylalanine ethyl ester + 1-butanol
?
show the reaction diagram
-
transesterification catalyzed by poly(ethylene glycol)-modified subtilisin
-
-
?
N-acetyl-L-phenylalanine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-Acetyl-Leu methyl ester + H2O
?
show the reaction diagram
-
subtilisin BPN'
-
-
?
N-Acetyl-Lys methyl ester + H2O
?
show the reaction diagram
-
subtilisin BPN'
-
-
?
N-Acetyl-Phe ethyl ester + H2O
?
show the reaction diagram
-
subtilisin BPN'
-
-
?
N-Acetyl-Phe methyl ester + H2O
?
show the reaction diagram
N-Acetyl-Trp ethyl ester + H2O
?
show the reaction diagram
-
subtilisin BPN'
-
-
?
N-Acetyl-Trp methyl ester + H2O
?
show the reaction diagram
N-Acetyl-Tyr ethyl ester + H2O
?
show the reaction diagram
N-acetyl-Tyr ethyl ester + H2O
N-acetyl-Tyr + ethanol
show the reaction diagram
-
-
-
?
N-Acetyl-Tyr methyl ester + H2O
?
show the reaction diagram
N-Acetyl-Val methyl ester + H2O
?
show the reaction diagram
N-acetylglucosamine + H2O
?
show the reaction diagram
N-CBZ-Gly-Gly-Leu p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
N-methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide + H2O
N-methoxysuccinyl-Ala-Ala-Pro-Val + p-nitroaniline
show the reaction diagram
N-Succ-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-Succ-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Met + p-nitroaniline
show the reaction diagram
relative hydrolysis rate is 39%
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Val-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
?
N-succinyl-L-Ala-L-Ala-L-L-Ala-4-nitroanilide + H2O
?
show the reaction diagram
N-succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
?
show the reaction diagram
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
?
show the reaction diagram
N-succinyl-L-Ala-L-Ala-L-Val-4-nitroanilide + H2O
?
show the reaction diagram
N-tosyl-L-Arg methyl ester + H2O
N-tosyl-L-Arg + methanol
show the reaction diagram
N-trans-cinnamoyl imidazole + H2O
(E)-cinnamate + imidazole
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-4-nitroanilide + H2O
Nalpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
Oxidized insulin B-chain + H2O
?
show the reaction diagram
oxidized insulin chain B + H2O
?
show the reaction diagram
-
is cleaved by subtilisin-like serine protease at multiple sites, preferably at the C-termini of the hydrophobic residues, such as Tyr, Phe, Leu, Val and Ala. This peptide is also cleaved by subtilisin at the C-termini of the hydrophobic residues, but at more specific sites
-
-
?
p-nitrophenyl butyrate + H2O
p-nitrophenol
show the reaction diagram
-
catalyzed by native subtilisin
-
-
?
peanut flour + H2O
?
show the reaction diagram
-
6 h alcalase hydrolysate
-
-
?
peptidyl-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
Phe-Ala-Ala-Phe-4-nitroanilide + H2O
Phe-Ala-Ala-Phe + 4-nitroaniline
show the reaction diagram
highly preferred substrate
-
-
?
porcine brain tubulin + H2O
peptides
show the reaction diagram
-
MALDI-mass spectrum of the carboxy-terminal peptides released by subtilisin treatment. Polyglutamylated peptides from the beta subunit and from both the tyrosinated and detyrosinated forms of the alpha subunit are observed. The fragments removed by subtilisin are in the range of 1.6 kDa. Post-translational modifications can be directly identified in the mixture of peptides resulting from limited subtilisin treatment
-
-
?
proCSF + H2O
CSF
show the reaction diagram
proPhrA + H2O
PhrA
show the reaction diagram
-
-
-
-
?
sec-phenethyl alcohol + vinyl butyrate
?
show the reaction diagram
-
-
-
-
?
serum albumin + H2O
?
show the reaction diagram
-
-
-
-
?
silk fibroin + H2O
?
show the reaction diagram
-
-
-
-
?
Spirulina platensis powder + H2O
?
show the reaction diagram
-
-
-
-
?
strobilurin fungicide + H2O
?
show the reaction diagram
-
-
-
-
?
Suc-Ala-Ala-Pro-Phe-4-methyl-coumaryl-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
Suc-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
succinyl-AAPA-p-nitroanilide + H2O
?
show the reaction diagram
succinyl-AAPE-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
succinyl-AAPF-4-nitroanilide + H2O
succinyl-AAPF + 4-nitroaniline
show the reaction diagram
-
-
-
?
succinyl-AAPF-p-nitroanilide + H2O
succinyl-AAPF + p-nitroaniline
show the reaction diagram
succinyl-AAPR-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
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Succinyl-Ala-Ala 4-nitroanilide + H2O
?
show the reaction diagram
Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
?
show the reaction diagram
succinyl-Ala-Ala-Ala-Ala-Ala-p-nitroanilide + H2O
succinyl-AAAAA + p-nitroaniline
show the reaction diagram
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?
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
succinyl-AAA + p-nitroaniline
show the reaction diagram
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?
succinyl-Ala-Ala-p-nitroanilide + H2O
succinyl-Ala-Ala + p-nitroaniline
show the reaction diagram
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?
succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
succinyl-Ala-Ala-Phe + 4-nitroaniline
show the reaction diagram
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-
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?
succinyl-Ala-Ala-Pro-Ala-p-nitroanilide + H2O
succinyl-AAPA + p-nitroaniline
show the reaction diagram
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?
succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
succinyl-AAPL + p-nitroaniline
show the reaction diagram
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?
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
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?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + p-nitroaniline
show the reaction diagram
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?
succinyl-Ala2-Phe-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Ala-4-nitroanilide + H2O
?
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O
succinyl-L-Ala-L-Ala-L-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-Gly + 4-nitroaniline
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Ala + 4-nitroaniline
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Met + 4-nitroaniline
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
?
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
show the reaction diagram
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?
succinyl-L-Ala-L-Ala-L-Pro-L-Tyr-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Tyr + 4-nitroaniline
show the reaction diagram
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?
succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O
succinyl-L-Asp-L-Val-L-Arg-L-Ala-L-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
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?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
succinyl-Phe-Ala-Ala-Phe-p-nitroanilide + H2O
succinyl-Phe-Ala-Ala-Phe + p-nitroaniline
show the reaction diagram
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?
Toluenesulfonyl-Arg methyl ester + H2O
?
show the reaction diagram
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subtilisin Carlsberg, subtilisin Novo
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type I keratin + H2O
?
show the reaction diagram
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?
ubiquitin + H2O
?
show the reaction diagram
Urea-denatured hemoglobin + H2O
?
show the reaction diagram
vinyl butyrate + H2O
?
show the reaction diagram
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?
Z-Ala-Ala-Leu-OCH3 + H2O
?
show the reaction diagram
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?
Z-Ala-Ala-Leu-OMe + Phe-p-nitroanilide
Z-Ala-Ala-Leu-Phe-p-nitroanilide + methanol
show the reaction diagram
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?
Z-Ala-Ala-Leu-p-nitroanilide + H2O
?
show the reaction diagram
Z-Ala-Phe-OMe + H2O
Z-Ala-Phe-NH2 + methanol
show the reaction diagram
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enzymatic method for the synthesis of free terminal amides of peptides, by ammonolysis of peptide methyl esters using ammonium carbamate and subtilisin A from Bacillus licheniformis in polar organic solvents with low water content is developed. Enzyme is very stable and active in a mixture of t-BuOH and DMF 82.5:17.5 (v/v), containing 0.2% water. Optimum conditions for Z-Ala-Phe-NH2 synthesis are molar ratio ammonium carbamate to Z-Ala-Phe-OMe 10, in t-BuOH/DMF, 82.5:17.5 (v/v) containing 0.2% (v) water, at 30°C for 21 h with the maximum yield of 87%
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Z-Thr-Ala-Thr-OCH3 + Asp-p-nitroanilide
Z-Thr-Ala-Thr-Asp-p-nitroanilide + methanol
show the reaction diagram
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additional information
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