3.4.21.57: Leucyl endopeptidase

This is an abbreviated version!
For detailed information about Leucyl endopeptidase, go to the full flat file.

Word Map on EC 3.4.21.57

Reaction

Hydrolysis of proteins. Preferential cleavage: Leu-/- in small molecule substrates =

Synonyms

Proteinase, leucine-specific, Leucine endopeptidase, Proteinase, spinach serine (leucine specific), Spinach leucine-specific serine proteinase, Plant Leu-proteinase, Leucine-specific serine proteinase, Leu-proteinase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.57 Leucyl endopeptidase

KM Value

KM Value on EC 3.4.21.57 - Leucyl endopeptidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.63
azocasein
-
-
-
0.315
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
-
50 mM Tris-HCl pH 8.0, 37°C, 10 min
0.25
Nalpha-benzoyl-Ala 4-nitroanilide
-
-
0.89
Nalpha-benzoyl-Gly 4-nitroanilide
-
-
0.47
Nalpha-benzoyl-L-Arg-4-nitroanilide
-
-
0.05
Nalpha-benzoyl-Leu 4-nitroanilide
-
-
0.45
Nalpha-benzoyl-Lys 4-nitroanilide
-
-
0.18
Nalpha-benzoyl-Val 4-nitroanilide
-
-
0.12
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester
-
-
0.28
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester
-
-
0.52
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester
-
-
0.065
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester
-
-
0.018
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester
-
-
0.26
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester
-
-
0.066
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester
-
-
additional information
additional information
-
Km values are pH-independent
-