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3.4.21.53: Endopeptidase La

This is an abbreviated version!
For detailed information about Endopeptidase La, go to the full flat file.

Word Map on EC 3.4.21.53

Reaction

hydrolysis of proteins in presence of ATP =

Synonyms

AAA+ Lon protease, AAA+ protease, AAAP, AF0364, AfLon, archaeal Lon protease, ATP-dependent lon protease, ATP-dependent Lon proteinase, ATP-dependent PIM1 protease, ATP-dependent protease La, ATP-dependent protease lon, ATP-dependent protease LonA, ATP-dependent serine proteinase, ATP-independent Lon-like protease, bacterial protease lon, BPP1347, ClpXP, EcLon, Ec-Lon, Ec-Lon protease, EcLon, EcLon protease, ELon, Escherichia coli proteinase La, Escherichia coli serine proteinase La, Gene lon protease, Gene lon proteins, hLon, human ATP-dependent protease, human lon protease, HVO_0783, i-AAA Protease, la, La protease, lon, lon (la) protease, lon (Pim1p) protease, Lon AAA+ protease, lon ATP-dependent protease, lon protease, LON protease 1, Lon protein, Lon proteinase, lon-like protease, Lon-like-Ms, lon1, lon2, lon3, lon4, lonA, lonB, lonB protease, LonC, LonC protease, lonD, LONP1, lonR9, LONRF1, lonS, lonTK, lonV, mitochondrial ATP-dependent protease, mitochondrial ATP-dependent protease La, mitochondrial Lon protease, MLon, Ms-Lon, Msm 1754, Msm_1754, MtaLonA, MtaLonC, Nmag_2822, NmLon, non-canonical RNA viral Lon proteinase, peroxisomal Lon protease, PIM1, PIM1 protease, PIM1 proteinase, Pim1p, PLon, protease, Protease La, protease lon, Proteinase La, Proteinase, Escherichia coli serine, La, Proteinase, La, Proteins, gene lon, Proteins, specific or class, gene lon, ScLon, serine protease, Serine protease La, Ta1081, Thela2p4_005149, Thela2p4_006664, TK1264, TonLonB, TON_0529, yeast mitochondrial lon, yeast protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.53 Endopeptidase La

Inhibitors

Inhibitors on EC 3.4.21.53 - Endopeptidase La

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',3'-dialdehyde-ATP
-
i.e. adenosine 2',3'-dialdehyde triphosphate, in the presence of ATP, globin as substrate
3,4-dichloroisocoumarin
-
-
Acetyl-Gly-Gly-Ala chloromethyl ketone
-
-
adenosine 5'-(3-thiotriphosphate)
-
i.e. ATP-gamma-S, in the presence of ATP, globin as substrate
Adenosine 5'-(betathio)triphosphate
-
-
adenosine 5'-[beta,gamma-imido]triphosphate
-
protein and ATP hydrolysis
adenyl-5'-yl methylene diphosphonate
-
i.e. AMP-PCP
Ala-Lys-Arg chloromethyl ketone
-
weak, casein as substrate
alpha,beta-methylene-ATP
-
in the presence of ATP, globin as substrate
anti-sense morpholino oligonucleotide
-
causes defects in mitochondrial membrane potential, respiration and morphology, as well as apoptotic cell death
-
azide
-
weak
bacteriophage T4 PinA protein
-
Bacteriophage T4 protease inhibitor PinA
-
-
-
Benzoyl-Arg-Gly-Phe-methoxynaphthylamide
-
-
Benzoyl-Arg-Gly-Phe-Phe-Leu-methoxynaphthylamide
-
glutaryl-Ala-Ala-Phe-methoxynaphthylamide or casein as substrate
benzyloxycarbonyl-Gly-Leu-Phe chloromethyl ketone
benzyloxycarbonyl-Gly-Leu-Pro chloromethyl ketone
-
-
Benzyloxycarbonyl-Gly-NH-C6H4SO2-Phe
-
weak
benzyloxycarbonyl-Phe chloromethyl ketone
beta,gamma-methylene-ATP
bortezomib
cardiolipin
-
cardiolipin-containing liposomes specifically inhibit both the proteolytic and ATPase activities of Lon in a dose-dependent manner. Cardiolipin-containing liposomes selectively bind to Lon. The interaction between cardiolipin-containing liposomes and Lon changes with the order of addition of Mg2+/ATP. When cardiolipin-containing liposomes are added after the addition of Mg2+/ATP to Lon, the binding of cardiolipin-containing liposomes to Lon is significantly decreased as compared with the reversed order
chymostatin
clasto-lactacystin beta-lactone
colicin
-
-
-
Dansyl fluoride
-
protein and ATP hydrolysis
dansyl-YRGIT-Abu
-
not time-dependent inhibitor of peptide hydrolysis activity of lon
dansyl-YRGIT-Abu-B(OH)2
Denatured lambda Cro protein
-
casein as substrate
-
diisopropyl fluorophosphate
dimethylformamide
-
above 5%
Dimethylsulfoxide
-
above 5%
Dio-9
-
ATPase inhibitor
-
ethanol
-
-
ethylenediaminetetraacetic acid
-
-
Glutaryl-Ala-Ala-Phe
-
ATP hydrolysis
Glutaryl-Ala-Ala-Phe-methoxynaphthylamide
glycerol
-
above 5%
H2O2
-
blocks enzymatic activity but has no appreciable effect on sequence-specific binding to DNA. 0.1 mM decreases proteolytic activity to ca. 60%, at 0.5, 1, and 4 mM protease activity is reduced to 18, 9, and 5%, respectively
heparin
-
strong
iodoacetamide
Isopropanol
-
above 5%
KCl
-
weak, above 0.2 M
lambda-N-Protein
-
casein as substrate
-
Mersalyl acid
-
strong, 5 mM, casein as substrate
MG 262
-
is a potent peptide-based inhibitor
MG132
Mg2+
excess of magnesium ions has an inhibitory effect on the hydrolysis of ATP
MG262
N,N'-dicyclohexylcarbodiimide
N-ethylmaleimide
oligomycin
p-nitroanthranilate
-
weak
Peptide substrates
-
inhibit ATP hydrolysis, protein substrates promote ATP hydrolysis
-
peroxynitrite
-
in isolated nonsynaptic brain mitochondria Lon protease is highly susceptible to oxidative inactivation by peroxynitrite. This susceptibility is more pronounced with regard to ATP-stimulated activity, which is inhibited by 75% in the presence of a bolus addition of 1 mM ONOO, whereas basal unstimulated activity is inhibited by 45%. A decline in Lon protease activity precedes electron transport chain dysfunction and ATP-stimulated activity is approximately fivefold more sensitive than basal Lon protease activity. Supplementation of mitochondrial matrix extracts with reduced glutathione, following peroxynitrite exposure, results in partial restoration of basal and ATP-stimulated activity
phenylmethylsulfonyl fluoride
phosphate
-
above 0.1 M
Polyphosphate
Protein R9 from Escherichia coli mutant strain capR9
-
protein, peptide and ATP hydrolysis
-
quercetin
RNAi
-
Succinyl-Phe-Ala-Phe-methoxynaphthylamide
-
ATP hydrolysis
TorD
-
impairs the enzyme's TorA degradation activity by binding to apoTorA
-
tosyl-Lys chloromethyl ketone
tosyl-Phe chloromethyl ketone
Triton X-100
-
0.05%
vanadate
additional information
-