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3.4.21.45: complement factor I

This is an abbreviated version!
For detailed information about complement factor I, go to the full flat file.

Word Map on EC 3.4.21.45

Reaction

Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage =

Synonyms

C3b inactivator, C3b/C4b inactivator, C3bINA, CFI, complement C3b inactivator, complement C3b/C4b inactivator, complement C4b inactivator, complement C4bi, complement compoment C3b inactivator, complement factor I, complement inhibitor factor I, complement regulator factor I, conglutinogen-activating factor C, factor I, factor I-like activity, fI, GcIf-1, GcIf-2, GcIf-3, GcIf-4, IF, plasma protease factor I, serine protease Factor I

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.45 complement factor I

Cloned

Cloned on EC 3.4.21.45 - complement factor I

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli
cloned into the eukaryotic expression vector pcDNA3 with addition of an N-terminal His-tag and transiently expressed in human embryonic kidney 293 cells
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expressed as an N-terminal His-tagged fusion protein in human embryonic kidney cells
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expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli, generation of mice strains deficient of complement factor I
expressed in Escherichia coli, mutant and unmodified proteins
expression of His6-tagged wild-type and mutant enzymes in HEK-293 cells
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HEK-293 cells transiently transfected with wild-type CFI or recombinant mutant constructs
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serine protease region of factor I flanked by the Xho I site and cloned into vector pCXN2L/FLAG-PI. Plasmid transformed into Escherichia coli C600 and amplified. Expression in CHO cells