3.4.21.25: cucumisin

This is an abbreviated version!
For detailed information about cucumisin, go to the full flat file.

Reaction

hydrolysis of proteins with broad specificity =

Synonyms

Cuc m 1, cucumisin, cucumisin homologue, More, protC1, protease C1, religiosin C, soybean protease C1

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.25 cucumisin

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Results	in table
207	AA Sequence
5	Application
1	CAS Registry Number
4	Cloned(Commentary)
2	Crystallization (Commentary)
5	General Stability
1	IC50 Value
42	Inhibitors
5	kcat/KM [mM/s]
3	Ki Value [mM]
33	KM Value [mM]
4	Localization
8	Metals/Ions
10	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
23	Organism
1	Oxidation Stability
4	PDB ID
10	pH Optimum
8	pH Range
7	pH Stability
2	pI Value
2	Posttranslational Modification
14	Purification (Commentary)
1	Reaction
1	Reaction Type
23	Reference
15	Source Tissue
8	Specific Activity [micromol/min/mg]
7	Storage Stability
126	Substrates and Products (Substrate)
4	Subunits
10	Synonyms
8	Temperature Optimum [°C]
3	Temperature Range [°C]
14	Temperature Stability [°C]
44	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.4.21.25 - cucumisin

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

11

2 entries

4 - 10

Cucumis trigonus

-

more than 80% of the initial activity after 20 h of incubation at 35°C between pH 4.0 and 10.0, rapid activity loss at pH 2 or pH 12 for 20 h at 35°C, casein substrate

670521

4 - 11.5

Ficus religiosa

-

the enzyme shows more than 90% activity when incubated at pH 4.5 to 11.5 for 30 min

732844

6 - 11

Cucumis melo

-

80% of the activity remains after after incubation

95355

7 - 10

Cucumis melo

-

most stable between pH 7 and pH 10, after 1 h incubation at 60°C

95351

8 - 11

Cucumis melo

-

most stable between pH 8 and pH 11, after 2 h incubation at 25°C

95351

11

Cucumis melo

-

retains about 80% of the activity

95358

11

Cucumis melo

-

1 h incubation at 60°C, the immobilized enzyme is more stable than the soluble, keeps 80% of the activity

95347