3.4.21.25: cucumisin
This is an abbreviated version!
For detailed information about cucumisin, go to the full flat file.
Word Map on EC 3.4.21.25
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3.4.21.25
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melon
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subtilisin
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subtilisin-like
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sarcocarp
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prince
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merrill
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serping1
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subtilase
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beta-conglycinin
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nutrition
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food industry
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analysis
- 3.4.21.25
- melon
- subtilisin
-
subtilisin-like
- sarcocarp
-
prince
-
merrill
-
serping1
-
subtilase
- beta-conglycinin
- nutrition
- food industry
- analysis
Reaction
hydrolysis of proteins with broad specificity =
Synonyms
Cuc m 1, cucumisin, cucumisin homologue, More, protC1, protease C1, religiosin C, soybean protease C1
ECTree
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Substrates Products
Substrates Products on EC 3.4.21.25 - cucumisin
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REACTION DIAGRAM
acetyl-Ala-Ala-Ala-p-nitroanilide + H2O
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1% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester
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-
?
benzoyl-Tyr-ethyl ester + H2O
benzoyl-Tyr + ethanol
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24% of the activity with benzyloxycarbonyl-Tyr-nitrophenyl ester
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-
?
benzyloxycarbonyl-Glu-Phe + H2O
benzyloxycarbonyl-Glu + Phe
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weakly hydrolyzed
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-
?
benzyloxycarbonyl-Tyr-4-nitrophenyl ester + H2O
benzyloxycarbonyl-Tyr + 4-nitrophenyl ester
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best synthetic substrate
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-
?
glutaryl-alanyl-alanyl-prolyl-leucine-4-nitroanilide + H2O
glutaryl-alanyl-alanyl-prolyl-leucine + 4-nitroaniline
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-
-
?
glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucyl-4-nitroanilide + H2O
glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucine + 4-nitroaniline
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-
-
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?
L-alanyl-L-alanyl-4-nitroanilide + H2O
L-alanyl-L-alanine + 4-nitroaniline
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-
-
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?
Leu-Leu-Leu-Leu-Pro-Glu-Ala-Leu + H2O
Leu-Leu-Leu-Leu-Pro-Glu + Ala-Leu
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-
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?
Leu-Leu-Pro-Arg-Ala-Leu + H2O
Leu-Leu-Pro-Arg + Ala-Leu
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low activity
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?
Leu-Leu-Pro-Glu-Ala-Leu + H2O
Leu-Leu-Pro-Glu + Ala-Leu
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low activity
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?
Leu-Leu-Pro-Glu-Ala-Leu-Leu-Leu + H2O
Leu-Leu-Pro-Glu + Ala-Leu-Leu-Leu
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-
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?
Leu-Leu-Pro-Glu-Glu-Leu + H2O
Leu-Leu-Pro-Glu + Glu-Leu
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low activity
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?
Leu-Leu-Pro-Glu-Phe-Leu + H2O
Leu-Leu-Pro-Glu + Phe-Leu
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low activity
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?
Leu-Leu-Pro-Lys-Ala-Leu + H2O
Leu-Leu-Pro-Lys + Ala-Leu
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low activity
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?
N-acetyl-L-alanyl-L-alanyl-alpha-azaalanine p-nitrophenyl ester + H2O
?
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-
-
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?
Protein + H2O
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-
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?
protein + H2O
hydrolyzed protein
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?
skim-milk powder + H2O
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milk-clotting activity, same activity as papain, EC 3.4.22.2 and half value of that of ficain, EC 3.4.22.3
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-
?
Suc-Ala-Ala-Ala-p-nitroanilide + H2O
Suc-Ala-Ala-Ala + p-nitroaniline
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-
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?
Suc-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
Suc-Ala-Ala-Pro-Leu + p-nitroaniline
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-
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?
Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
Suc-Ala-Ala-Pro-Phe + p-nitroaniline
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-
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?
TMA-lysozyme BrCN fragment + H2O
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cleaves at two sites, Cys-Lys and Arg-Gly
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-
?
Benzyloxycarbonyl-Glu-Tyr + H2O
Benzyloxycarbonyl-Glu + Tyr
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weakly hydrolyzed
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?
?
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broad substrate specificity, but the bonds of the valine residue are not split
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-
?
Insulin B-chain + H2O
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three cleavage sites are detected after a reaction period of 30 s, Leu-Cys, Cys-Gly, and Glu-Ala
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-
?
Insulin B-chain + H2O
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wide specificity in hydrolysis of reduced and carboxymethylated insulin B-chain, the carboxyl side of bonds containing acidic amino acid residues such as CM-cysteine and glutamic acid is appreciably cleaved by the enzyme
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?
Leu-Leu-Pro-Met-Ala-Leu + H2O
Leu-Leu-Pro-Met + Ala-Leu
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best substrate
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?
?
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no hydrolysis of benzyloxycarbonyl-Ala-Ala-Ile-SCH2C6H5, benzyloxycarbonyl-Ala-Ala-Pro-SCH2C6H5
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additional information
?
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no hydrolysis of Lys-p-nitroanilide, Tyr-p-nitroanilide, Leu-p-nitroanilide, tosyl-Arg-methyl ester, acetyl-Phe-ethyl ester, benzyloxycarbonyl-Lys-nitrophenyl ester and benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide
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?
additional information
?
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the substrate specificity of the 67000 Da and the 54000 Da enzyme is almost the same
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?
additional information
?
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no hydrolysis of acetyl-L-tyrosine ethyl ester, benzoyl-L-arginine ethyl ester, benzoyl-L-arginine-p-nitroanilide, benzoyl-L-tyrosine-p-nitroanilide, glutathione, glycyl-L-leucine, Gly-Gly-Gly and Leu-Gly-Gly
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?
additional information
?
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substrate specificity at positions P6-P'4, no activity with Leu-Leu-Pro-Glu-Val-Leu, Pro-Glu-Ala-Leu, Leu-Pro-Glu-Ala, Leu-Leu-Pro-Gly-Ala-Leu, Leu-Leu-Pro-Ile-Ala-Leu and Leu-Leu-Pro-Pro-Ala-Leu, poor substrates are Leu-Leu-Pro-Tyr-Ala-Leu, Leu-Leu-Pro-Glu-Leu-Leu and Leu-Leu-Pro-Val-Ala-Leu
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?
additional information
?
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no activity toward Nalpha-benzoyl-DL-arginyl-4-nitroanilide and N-succinyl-L-phenylalanyl-4-nitroanilide
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?
additional information
?
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subtilisin-like serine protease initiating proteolysis of seed storage proteins during germination and early seedling growth
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?