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3.4.21.22: coagulation factor IXa

This is an abbreviated version!
For detailed information about coagulation factor IXa, go to the full flat file.

Word Map on EC 3.4.21.22

Reaction

Selective cleavage of Arg-/-Ile bond in factor X to form factor Xa =

Synonyms

activated Christmas factor, activated coagulation factor IX, activated factor IX, activated FIX, blood coagulation factor IXa, Christmas factor, circulating factor IXa, coagulation factor IX, coagulation factor IXa, factor IX, factor IXa, factor IXaAL, factor IXaalpha, factor IXabeta, factor IXabeta', factor IXaCH, factor IXalphabeta, factor IXaN, factor XIa, FIX, FIXa, FIXC, Gla-domainless factor IXabeta', human coagulation factor IXa, intrinsic Xase, More

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.22 coagulation factor IXa

Purification

Purification on EC 3.4.21.22 - coagulation factor IXa

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
combination of anion exchange and affinity/cation exchange chromatography
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DEAE-Sepharose column chromatography and HiTrap Q column chromatography
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factor IXabeta'
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gel filtration
heparin Sepharose column chromatography and Q Sepharose column chromatography
HiPrep Q column chromatography and Superdex 75 gel filtration
HiTrap Q column chromatography and HiTrap heparin column chromatography
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ion exchange chromatography on Q-Sepharose
ion-exchange chromatography
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method not mentioned
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Ni-NTA column chromatography, gel filtration
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Q-Sepharose of column, affinity purification with heparin sepharose, gel filtration
recombinant factor IXa
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recombinant fIXa
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recombinant wild-type and mutant enzymes from HEK-293 cells by anion exchange and heparin affinity chromatography
recombinant wild-type and mutant enzymes from HEK-293 cells by immunoaffinity and anion exchange chromatogaphy
recombinant wild-type and mutant factor IXa, immunoaffinity chromatography using HPC4 monoclonal antibodies