3.4.21.21: coagulation factor VIIa
This is an abbreviated version!
For detailed information about coagulation factor VIIa, go to the full flat file.
Word Map on EC 3.4.21.21
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3.4.21.21
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bleeding
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fibrinogen
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platelet
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viii
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hemostatic
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hemorrhage
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thrombosis
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anticoagulant
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thromboplastin
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plasminogen
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haemophilia
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antithrombin
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coagulopathy
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coronary
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transfusion
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willebrand
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thromboembolic
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trauma
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fibrin
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fibrinolysis
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haemostasis
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d-dimers
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antithrombotic
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warfarin
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hypercoagulable
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intravascular
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pai-1
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off-label
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perioperative
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hematoma
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k-dependent
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amidolytic
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thrombogenic
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antifibrinolytic
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tranexamic
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prothrombotic
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thrombin-antithrombin
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plasma-derived
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drug development
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prothrombinase
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epistaxis
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fondaparinux
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desmopressin
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cryoprecipitate
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thromboelastometry
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dabigatran
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biotechnology
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gamma-carboxyglutamic
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menorrhagia
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apixaban
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thromboelastography
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bethesda
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medicine
- 3.4.21.21
- bleeding
- fibrinogen
- platelet
- viii
-
hemostatic
- hemorrhage
- thrombosis
-
anticoagulant
- thromboplastin
- plasminogen
- haemophilia
- antithrombin
- coagulopathy
- coronary
-
transfusion
- willebrand
-
thromboembolic
- trauma
- fibrin
-
fibrinolysis
-
haemostasis
-
d-dimers
-
antithrombotic
- warfarin
-
hypercoagulable
-
intravascular
- pai-1
-
off-label
-
perioperative
- hematoma
-
k-dependent
-
amidolytic
-
thrombogenic
-
antifibrinolytic
-
tranexamic
-
prothrombotic
-
thrombin-antithrombin
-
plasma-derived
- drug development
- prothrombinase
- epistaxis
- fondaparinux
- desmopressin
-
cryoprecipitate
-
thromboelastometry
- dabigatran
- biotechnology
-
gamma-carboxyglutamic
- menorrhagia
- apixaban
-
thromboelastography
-
bethesda
- medicine
Reaction
Selective cleavage of Arg-/-Ile bond in factor X to form factor Xa =
Synonyms
activated blood coagulation factor VII, activated coagulation factor VII, activated factor VII, blood coagulation factor VIIa, blood-coagulation factor VII, activated, blood-coagulation factor VIIa, coagulation factor VII, coagulation factor VII(a), coagulation FVII, Eptacog alfa, Factor VII, factor VIIa, factor VIIa-sTF, factor VIIa-TF, factor VIIa/tissue factor complex, FIIa, free factor VIIa, FVII, FVIIa, FVIIa-sTF, fVIIa/TF, FVIIc, human factor VIIa + TF, More, NovoSeven, procoagulant protein factor VII, recombinant factor VIIa, recombinant factor-activated VII, rFVII, rFVIIa, serine protease factor VIIa, Serum prothrombin conversion accelerator, soluble tissue factor/factor VIIa complex, sTF/VIIa, TF-FVIIa, TF/VIIa, tissue factor/factor VIIa, tissue factor/factor VIIa complex, vitamin K dependent clotting factor
ECTree
3.4.21.21 coagulation factor VIIaAdvanced search results
results (
results (Turnover Number
Turnover Number on EC 3.4.21.21 - coagulation factor VIIa
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
17
methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide
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wild-type enzyme
13
S-2288
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in 50 mM HEPES, pH 7.4, with 0.1 M NaCl, 5 mM CaCl2, 0.1% (w/v) bovine serum albumin, and 0.01% Tween 80, at 37°C
7.7
D-Ile-Pro-Arg-4-nitroanilide
wild type enzyme, in the absence of soluble tissue factor (1-219), at pH 7.4 and 25°C
34.6
D-Ile-Pro-Arg-4-nitroanilide
wild type enzyme, in the presence of soluble tissue factor (1-219), at pH 7.4 and 25°C
0.59
D-Ile-Pro-Arg-p-nitroanilide
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pH 8.0, 30°C, mutant enzyme DELTA360-329, without tissue factor
1.95
D-Ile-Pro-Arg-p-nitroanilide
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pH 8.0, 30°C, activated wild-type enzyme, without tissue factor
3.29
D-Ile-Pro-Arg-p-nitroanilide
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pH 8.0, 30°C, plasma derived wild-type enzyme, without tissue factor
7.81
D-Ile-Pro-Arg-p-nitroanilide
-
pH 8.0, 30°C, mutant enzyme DELTA360-329, activated by tissue factor
18.53
D-Ile-Pro-Arg-p-nitroanilide
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pH 8.0, 30°C, mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin, without tissue factor
20.98
D-Ile-Pro-Arg-p-nitroanilide
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pH 8.0, 30°C, mutant enzyme which has all of the residues of the loop formed by a disulfide bond between Cys310 and Cys329 replaced with those of trypsin, activated by tissue factor
37.08
D-Ile-Pro-Arg-p-nitroanilide
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pH 8.0, 30°C, activated wild-type enzyme, activated by tissue factor
44.2
D-Ile-Pro-Arg-p-nitroanilide
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pH 8.0, 30°C, plasma derived wild-type enzyme, activated by tissue factor
0.00001
Factor X
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in 50 mM HEPES, pH 7.4, with 0.1 M NaCl, 5 mM CaCl2, 0.1% (w/v) bovine serum albumin, and 0.01% Tween 80, at 25°C
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0.00062
Factor X
mutant G372A + soluble tissue factor for induced allosteric activation of factor VIIa, pH 7.4
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0.0021
Factor X
mutant G372A + lipidated tissue factor for induced allosteric activation of factor VIIa, pH 7.4
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0.0027
Factor X
wild-type FVIIa + soluble tissue factor for induced allosteric activation of factor VIIa, pH 7.4
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0.004
Factor X
wild-type FVIIa + lipidated tissue factor for induced allosteric activation of factor VIIa, pH 7.4
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0.05
Factor X
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mutant enzyme L144A, tissue factor/phosphatidylcholine vesicles
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0.15
Factor X
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mutant enzyme L192A or E154A, tissue factor/phosphatidylcholine vesicles
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0.15
Factor X
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mutant enzyme L144A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles
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0.8
Factor X
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mutant enzyme K192A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles
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1.25
Factor X
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mutant enzyme E154A, tissue factor/phosphatidylcholine-phosphatidylserine vesicles
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1.55
Factor X
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wild-type enzyme VIIa, tissue factor/phosphatidylcholine vesicles
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7.4
Factor X
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wild-type enzyme VIIa, tissue factor/phosphatidylcholine-phosphatidylserine vesicles
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8.4
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 8.0, wild-type enzyme, without tissue factor
12.1
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 8.0, 37°C, wild-type enzyme
24.6
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 8.0, 37°C, mutant enzyme M156Q
32.2
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 8.0, mutant enzyme V21N/E154I/M156Q, without tissue factor
37.2
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 8.0, wild-type enzyme, with addition of tissue factor
47
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 8.0, mutant enzyme V21N/E154I/M156Q, with addition of tissue factor
49.1
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 8.0, 37°C, wild-type enzyme, addition of tissue factor
49.8
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 8.0, 37°C, mutant enzyme M156Q, addition of tissue factor
1500
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 6.5, MTE buffer, without tissue factor
1560
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 6.5, MES buffer, without tissue factor
5940
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 9.5, BTP buffer, without tissue factor
7200
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 9.5, ethanolamine buffer, without tissue factor
9600
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 9.5, MTE buffer, without tissue factor
31800
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 7.5, Tris buffer, without tissue factor
33600
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 7.5, HEPES buffer, without tissue factor
38400
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 7.5, MTE buffer, without tissue factor
84000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 9.5, veronal buffer, without tissue factor
96000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 6.5, MES buffer, in presence of tissue factor
96000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 6.5, MTE buffer, in presence of tissue factor
198000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 7.5, HEPES buffer, in presence of tissue factor
216000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 7.5, MTE buffer, in presence of tissue factor
246000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 7.5, Tris buffer, in presence of tissue factor
324000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 9.5, veronal buffer, in presence of tissue factor
360000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 9.5, MTE buffer, in presence of tissue factor
366000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
-
pH 9.5, BTP buffer, in presence of tissue factor
396000
N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide
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pH 9.5, ethanolamine buffer, in presence of tissue factor
additional information
additional information
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turnover-number of wild-type and mutant enzymes
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additional information
additional information
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the ratio of turnover number to KM-value for the substrate methanesulfonyl-D-cyclohexylalanyl-butyl-arginine p-nitroanilide is 25000 for wild-type enzyme, 500 for mutant enzyme V154G and 2500 for mutant enzyme V154A
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