3.4.21.19: glutamyl endopeptidase

This is an abbreviated version!
For detailed information about glutamyl endopeptidase, go to the full flat file.

Reaction

Preferential cleavage: Glu-/-, Asp-/- =

Synonyms

Alcalase, BIEP, BIGEP, BL-GSE, BLase, BS-GSE, endoproteinase Glu-C, endoproteinase V8, Glu C, Glu-C, Glu-endopeptidase, Glu-specific endopeptidase, GluScoh, GluScpr, GluSE, GluSsap, GluSW, glutamate specific endopeptidase, glutamate-specific proteinase, glutamate-specific serine endopeptidase, glutamic acid-specific endopeptidase, glutamic acid-specific proteinase, glutamic-acid-specific endopeptidase, glutamyl endopeptidase, glutamyl endopeptidase 2, glutamyl endopeptidase I, glutamyl specific endopeptidase, GluV8, GSE, gseBi gene product, protease V8, proteinase, glutamate-specific, proteinase, staphylococcal serine, SG-GSE, SGPE, Sspa, staphylococcal serine proteinase, V8 protease, V8 proteinase, V8-GSE, V8-protease, VSPase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.19 glutamyl endopeptidase

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Results	in table
5	Activating Compound
145	AA Sequence
9	Application
2	CAS Registry Number
19	Cloned(Commentary)
5	Crystallization (Commentary)
2	General Stability
32	Inhibitors
1	kcat/KM [mM/s]
38	KM Value [mM]
19	Localization
10	Metals/Ions
19	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
165	Organism
19	PDB ID
31	pH Optimum
2	pH Range
3	pH Stability
2	pI Value
4	Posttranslational Modification
40	Protein Variants
22	Purification (Commentary)
8	Reaction
4	Reaction Type
82	Reference
1	Renatured (Commentary)
6	Source Tissue
13	Specific Activity [micromol/min/mg]
3	Storage Stability
226	Substrates and Products (Substrate)
22	Subunits
102	Synonyms
19	Temperature Optimum [°C]
8	Temperature Stability [°C]
32	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.4.21.19 - glutamyl endopeptidase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

22

Bacillus intermedius

-

pH 6.5-11.0, 3 h, stable

81317

22 - 37

Bacillus intermedius

-

stable, in absence of Ca2+

650274

37 - 55

Bacillus intermedius

-

stable, in presence of Ca2+

650274

50

3 entries

55

Bacillus intermedius

-

in absence of Ca2+, loss of 90% activity

650274

60 - 70

Thermoactinomyces sp. CDF

-

in the presence of 10 mM CaCl2, the enzyme does not show loss of activity after incubation at 60°C for 6 h and retains approximately 90% of its original activity after incubation at 70°C for 6 h

752559

50

Bacillus intermedius

-

pH 8.0, 24 h, in presence of 5 mM Ca2+, stable

81317

50

Bacillus intermedius

-

in absence of Ca2+, loss of 55% activity

650274

50

Bacillus intermedius

-

mutant H186T, 150 min, no loss of activity

670744