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3.4.21.19: glutamyl endopeptidase

This is an abbreviated version!
For detailed information about glutamyl endopeptidase, go to the full flat file.

Word Map on EC 3.4.21.19

Reaction

Preferential cleavage: Glu-/-, Asp-/- =

Synonyms

Alcalase, BIEP, BIGEP, BL-GSE, BLase, BS-GSE, endoproteinase Glu-C, endoproteinase V8, Glu C, Glu-C, Glu-endopeptidase, Glu-specific endopeptidase, GluScoh, GluScpr, GluSE, GluSsap, GluSW, glutamate specific endopeptidase, glutamate-specific proteinase, glutamate-specific serine endopeptidase, glutamic acid-specific endopeptidase, glutamic acid-specific proteinase, glutamic-acid-specific endopeptidase, glutamyl endopeptidase, glutamyl endopeptidase 2, glutamyl endopeptidase I, glutamyl specific endopeptidase, GluV8, GSE, gseBi gene product, protease V8, proteinase, glutamate-specific, proteinase, staphylococcal serine, SG-GSE, SGPE, Sspa, staphylococcal serine proteinase, V8 protease, V8 proteinase, V8-GSE, V8-protease, VSPase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.19 glutamyl endopeptidase

Cloned

Cloned on EC 3.4.21.19 - glutamyl endopeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a proform that contains a 61-amino acid N-terminal propeptide and a 218-amino acid mature domain are expressed in Escherichia coli BL21(DE3) cells
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different BIGEP forms (full-length precursor, precursor without signal peptide and mature part) are expressed in Escherichia coli
expressed as soluble His-tag fusion protein in Escherichia coli
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expressed in Bacillus subtilis recombinant strains with mutations in the regulatory proteins Spo0A, KinA, and Ger
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expressed in enzyme deficient strain Bacillus subtilis AJ73, sporulation and enzyme expression is strongly reduced when 1% glucose is added to the culture medium at the beginning of the growth phase, no effect of glucose addition after 20 h of cultivation period, slightly enhanced activity when glucose is added after 30 h of cultivation
expressed in Escherichia coli
expressed in Escherichia coli, poor expression of native full length protein, successful expression when mature protein sequence is fused with the pro-sequence of the analogous enzyme from Staphylococcus epidermidis, successful expression of double and four point mutant in Escherichia coli
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expression in Bacillus subtilis, optimization of culture medium composition for production of recombinant enzyme, recombinant enzyme is secreted to the medium from Bacillus subtilis cells, 2-5% of the enzyme remain bound to the cell wall
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