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3.4.17.21: Glutamate carboxypeptidase II

This is an abbreviated version!
For detailed information about Glutamate carboxypeptidase II, go to the full flat file.

Word Map on EC 3.4.17.21

Reaction

release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates =

Synonyms

100 kDa ileum brush border membrane protein, Acetylaspartylglutamate dipeptidase, altered meristem program1, AMP1, Dipeptidase, acetylaspartylglutamate, EC 3.4.19.8, FGCP, folate hydrolase, folate hydrolase 1, FOLH1, Folylpoly-gamma-glutamate carboxypeptidase, folylpoly-gamma-glutamate carboxypeptidase II, GCP II, GCPII, GCPIII, glutamate carboxypeptidase, glutamate carboxypeptidase II, glutamate carboxypeptidase III, GPCPII, I100, Ileal dipeptidylpeptidase, Membrane glutamate carboxypeptidase, mGCP, More, N-acetyl-alpha-linked acidic dipeptidase, N-acetyl-alpha-linked acidic dipeptidase I, N-acetylaspartylglutamate peptidase, N-acetylated alpha-linked acid dipeptidase, N-Acetylated alpha-linked acidic dipeptidase, N-Acetylated-alpha-linked acidic dipeptidase, N-acetylated-alpha-linked acidic dipeptidase 2, N-acetylated-alpha-linked acidic dipeptidase II, N-Acetylated-alpha-linked-acidic dipeptidase, N-acetylated-alpha-linked-acidic-dipeptidase, N-Acetylated-alpha-linked-amino dipeptidase, NAADLADase, NAADLADse, NAAG degradation enzyme, NAAG peptidase, NAAG peptidase II, NAAG-hydrolyzing activity, NAALA dipeptidase, NAALADase, Naaladase I, NAALADase II, NLD I, PMSA, prostate specific membrane antigen, Prostate-specific membrane antigen, Prostate-specific membrane antigen homolog, prostate-specificmembrane antigen, Prostrate-specific membrane antigen, PSM, PSM antigen, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase, Rat NAAG peptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.21 Glutamate carboxypeptidase II

Cloned

Cloned on EC 3.4.17.21 - Glutamate carboxypeptidase II

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 20.7 kb gene fragment encoding exons 1-3 is subcloned and analyzed by restriction mapping
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cloning and expression of the His6-tagged extracellular portion of the enzyme in High5 cells using the baculovirus transfection system, secretion of the glycosylated extracellular domain
construction of the DNA vaccines carrying truncated human tPSMA gene driven by the cytomegalovirus promoter and mouse 4-1BBL gene driven by an IRES
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expression in CHO cells
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expression in COS-1 cells, mannose-rich glycosylation of the recombinant enzyme in absence of an inhibitor, recombinant glycosylation and subcellular transport analysis, overview, stable expression in MDCK cells at the cell surface
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expression in Drosophila S2 cells
expression in DU145 cell line
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expression in PC3 cell
expression of the full-length enzyme and the mutant enzymes DEALTA20-42 and DELTA1-42 in HEK293 cells
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expression of wild-type and mutant enzymes in PC3 cells
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for expression in Drosophila Schneiders S2 cells
gene FOLH1, DNA sequence variants analysis, expression regulation by PMSA enhancer, PSME, involving Ca2+, overview, localization on chromosome 11p11-12, another highly homologous gene is located at 11q14.3 and is expressed in several tissues, e.g. kidney and liver, but not in prostate
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gene Naalad2, DNA and amino acid sequence determination and analysis, expression of GCPIII in CHO cells
gene PMSA, DNA sequence variants analysis, expression regulation by PMSA enhancer, PSME, involving Ca2+, overview
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genetic structure, overview
recombinant expression
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stable expression in MDCK cells of truncated PMSA-DELTA103-750 as GFP-fusion protein and of the extracellular domain
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stable expression of wild-type and mutant enzymes in S2 cells, secretion of the enzyme, which is prevented by blocking the N-glycosylation
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the extracellular part, amino acids 44-750, of human GCPII is cloned and heterologously overexpressed in Drosophila Schneiders S2 cells
the gene is homologuos to the prostrate-specific membrane antigen
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