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3.4.17.20: Carboxypeptidase U

This is an abbreviated version!
For detailed information about Carboxypeptidase U, go to the full flat file.

Word Map on EC 3.4.17.20

Reaction

release of C-terminal Arg and Lys from a polypeptide =

Synonyms

aCAP, activated thrombin activable fibrinolysis inhibitor, activated thrombin-activable fibrinolysis inhibitor, activated thrombin-activatable fibrinolysis inhibitor, active carboxypeptidase B, active thrombin-activatable fibrinolysis inhibitor, Arginine carboxypeptidase, Carboxypeptidase B, pro-, carboxypeptidase B2, Carboxypeptidase R, carboxypeptidase U, Carboxypeptidase-U, CPB2, CPR, CPU, Plasma carboxypeptidase B, plasma carboxypeptidase U, plasma CPB, plasma procarboxypeptidase B, plasma procarboxypeptidase B-like proenzyme, plasma procarboxypeptidase U, pro-pCPB, Procarboxypeptidase B, procarboxypeptidase R, procarboxypeptidase U, proCPU, pTAFI, rTAFI, TAFI, TAFIa, TFAI, thrombin activable fibrinolysis inhibitor, thrombin activatable fibrinolysis inhibitor, Thrombin-activable fibrinolysis inhibitor, thrombin-activatable fibrinolysis inhibitor

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.20 Carboxypeptidase U

Purification

Purification on EC 3.4.17.20 - Carboxypeptidase U

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
development of a method to isolate in situ activated TAFIa from human serum in presence of epsilon-aminocaproic acid
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development of a method to isolate in situ activated TAFIa from serum in presence of epsilon-aminocaproic acid
from plasma
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from plasma by a plasminogen affinity based method
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native pro-CPU from human plasma by two steps of ion exchange chromatography, plasminogen affinity chromatography, gel filtration, and anion exchange chromatography, recombinant pro-CPU from Sf21 and H5 insect cells using the same method as for the native enzyme, to homogeneity
of cloned enzyme from BHK-cells
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purified by chromatography on Q-Sepharose Fast Flow, Heparin-Sepharose CL-6B, Sephacryl S-300, and plasminogen-Sepharose columns
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purified from plasma using a ECH-lysine sepharose column and anion-exchange chromatography
recombinant enzyme and mutant enzyme R302Q
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recombinant TAFI and TAFI/CPB(carboxypeptidase B) chimeras
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recombinant wild-type TAFI isozymes Thr147 and Thr325 and TAFI-carboxypeptidase B chimeric proteins from baby hamster kidney cells by immunoaffinity and ion exchange chromatography
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using a Nik-9H10-Sepharose column, coupled with the antibody Nig9H10 whose epitope is located on the activation peptide of TAFI
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using immunoaffinity chromatography in which monoclonal antibody MA-T4E3 is coupled to CNBr-activated Sepharose 4B
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wild-type and mutant enzymes
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