3.4.17.2: carboxypeptidase B

This is an abbreviated version!
For detailed information about carboxypeptidase B, go to the full flat file.

Word Map on EC 3.4.17.2

Reaction

preferential release of a C-terminal lysine or arginine amino acid =

Synonyms

More, TAFI, EC 3.4.2.2, EC 3.4.12.3, protaminase, carboxypeptidase BII, CPB, Cbp, 47 kDa zymogen granule membrane associated protein, Pancreas-specific protein, PASP, ZAP47, PCB, CPB1, thrombin-activatable fibrinolysis inhibitor, HBCPB, PcpB, aCAP, tissue carboxypeptidase B, basic carboxypeptidase, pp-CpB, B carboxypeptidase, CPBAg1, CPB2, carboxypeptidase B, thrombin-activatable procarboxypeptidase B, Carboxypeptidase B1, HzCPB, cpbAs1, ppCPB, carboxypeptidase B2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.2 carboxypeptidase B

Substrates Products

Substrates Products on EC 3.4.17.2 - carboxypeptidase B

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(2-furyl)acryloyl-L-Ala-L-Arg + H2O
3-(2-furyl)acryloyl-L-Ala + L-Arg
show the reaction diagram
-
-
-
?
Arg-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
?
show the reaction diagram
-
bradykinin
-
-
?
benzoyl-beta-Ala-L-Lys + H2O
?
show the reaction diagram
-
low activity
-
-
?
benzoyl-Gly-Arg + H2O
benzoyl-Gly + Arg
show the reaction diagram
benzoyl-Gly-Gly-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-glycylphenyllactic acid + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Gly-L-Arg + H2O
benzoyl-Gly + L-Arg
show the reaction diagram
-
-
-
-
?
biotin-(epsilon-aminocaproic acid)2-GLMVGGVVR + H2O
biotin-(epsilon-aminocaproic acid)2-GLMVGGVV + Arg
show the reaction diagram
-
-
-
?
carbobenzoxy-Gly-L-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
endostar + H2O
?
show the reaction diagram
-
endostar is a derivative of human endostatin that is modified with an additional metal-chelating sequence at the N-terminus. A mixture of carboxypeptidase A and carboxypeptidase B is applied to catalyse the hydrolysis of the C-terminus of apo and holo endostar
-
-
?
Fibrin + H2O
?
show the reaction diagram
-
CPB activity generates fine-mesh fibrin which is more difficult to lyse by tPA
-
-
?
furylacryloyl-L-alanyl-L-arginine + H2O
furylacryloyl-L-alanine + L-arginine
show the reaction diagram
-
-
-
-
?
furylacryloyl-L-alanyl-L-lysine + H2O
furylacryloyl-L-alanine + L-lysine
show the reaction diagram
-
-
-
-
?
furylacryloylalanylarginine + H2O
?
show the reaction diagram
-
-
-
-
?
furylacryloylalanyllysine + H2O
?
show the reaction diagram
-
-
-
-
?
hippuryl-Gly + H2O
?
show the reaction diagram
-
10% of the activity with hippuryl-L-Arg
-
-
?
hippuryl-L-Arg
hippuric acid + L-Arg
show the reaction diagram
Hippuryl-L-Arg + H2O
Hippuric acid + L-Arg
show the reaction diagram
hippuryl-L-arginine + H2O
hippuric acid + L-Arg
show the reaction diagram
-
-
-
?
hippuryl-L-Asp + H2O
hippuric acid + L-aspartate
show the reaction diagram
-
hydrolyzed by mutant enzyme D253K and mutant enzyme D253R, wild type enzyme shows no activity
-
-
?
hippuryl-L-Glu + H2O
hippuric acid + Glu
show the reaction diagram
-
hydrolyzed by mutant enzyme D253K, wild type enzyme shows no activity
-
-
?
hippuryl-L-homoarginine + H2O
hippuric acid + homoarginine
show the reaction diagram
-
low activity
-
-
?
hippuryl-L-Lys
hippuric acid + L-Lys
show the reaction diagram
-
-
-
?
hippuryl-L-Orn + H2O
hippuric acid + Orn
show the reaction diagram
-
-
-
-
?
hippuryl-L-Phe + H2O
?
show the reaction diagram
-
18% of the activity with hippuryl-L-Arg
-
-
?
hippuryl-L-Pro-L-Lys + H2O
?
show the reaction diagram
-
low activity
-
-
?
hippuryl-Lys + H2O
hippuric acid + Lys
show the reaction diagram
hippurylphenyllactic acid + H2O
hippuric acid + phenyllactic acid
show the reaction diagram
-
-
-
-
?
human insulin-like peptide 3 + H2O
?
show the reaction diagram
-
the single-chain precursor of INSL3 is converted to the double-chain mature human INSL3 by endoproteinase Lys-C and carboxypeptidase B treatment. Carboxypeptidase B is added (emzyme/peptide mass ratio 1 : 30) to remove the additional lysine residue at the C-terminus of B-chain
-
-
?
human proinsulin + H2O
?
show the reaction diagram
-
enzymatic modification of human proinsulin using trypsin and carboxypeptidase B generally causes high accumulation of insulin derivatives, leading to more complicated purification processes. A simple method including citraconylation and decitraconylation in the enzymatic modification process is developed for the reduction of a major derivative, des-threonine human insulin
-
-
?
insulin-like peptide 5 precursor + H2O
?
show the reaction diagram
-
-
-
-
?
Leu-Asp-Ser + H2O
Leu-Asp + Ser
show the reaction diagram
-
-
-
?
mono/di-Arg-insulin + H2O
human insulin
show the reaction diagram
-
-
-
-
?
N-(4-methoxyphenylazoformyl)-Arg + H2O
?
show the reaction diagram
N-(4-methoxyphenylazoformyl)-Arg-OH + H2O
?
show the reaction diagram
-
-
-
-
?
N-(4-methoxyphenylazoformyl)-Phe + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzoyl-Gly-L-Arg + H2O
N-benzoyl-Gly + L-Arg
show the reaction diagram
OPN-R + H2O
OPN-L + ?
show the reaction diagram
-
osteopontin (OPN) gets activated by thrombin resulting in OPN-R. OPN-R is subsequently inactivated by CPB generating OPN-L
-
-
?
Val-Asp-Asp-Asp-Lys + H2O
Lys + Asp + ?
show the reaction diagram
-
-
-
?
additional information
?
-