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3.4.17.2: carboxypeptidase B

This is an abbreviated version!
For detailed information about carboxypeptidase B, go to the full flat file.

Word Map on EC 3.4.17.2

Reaction

preferential release of a C-terminal lysine or arginine amino acid =

Synonyms

47 kDa zymogen granule membrane associated protein, aCAP, B carboxypeptidase, basic carboxypeptidase, carboxypeptidase B, Carboxypeptidase B1, carboxypeptidase B2, carboxypeptidase BII, Cbp, CPB, CPB1, CPB2, CPBAg1, cpbAs1, EC 3.4.12.3, EC 3.4.2.2, HBCPB, HzCPB, More, Pancreas-specific protein, PASP, PCB, PcpB, pp-CpB, ppCPB, protaminase, TAFI, thrombin-activatable fibrinolysis inhibitor, thrombin-activatable procarboxypeptidase B, tissue carboxypeptidase B, ZAP47

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.2 carboxypeptidase B

Purification

Purification on EC 3.4.17.2 - carboxypeptidase B

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
from gut by affinity chromatography
further purification of the commercial pancreatic preparation by hydrophobic interaction chromatography
large-scale
-
multiple forms: carboxypeptidase B I and B II
-
native enzyme 418fold from pyloric ceca to near homogeneity by gel filtration, anion exchange chromatography, and another step of gel filtration
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native enzyme from pancreatic acetine powder
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native proenzyme from pancreatic juice, separation of active enzyme and inactive proenzyme by gel filtration
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procarboxypeptidase B
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purified by chromatography on Q-Sepharose Fast Flow, Heparin-Sepharose CL-6B, Sephacryl S-300, and plasminogen-Sepharose columns
-
using affinity chromatography and gel filtration
using ammonium sulfate precipitation and gel filtration
-
using Ni-NTA chromatography