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3.4.14.2: dipeptidyl-peptidase II

This is an abbreviated version!
For detailed information about dipeptidyl-peptidase II, go to the full flat file.

Word Map on EC 3.4.14.2

Reaction

release of an N-terminal dipeptide, Xaa-Yaa-/-, preferentially when Yaa is Ala or Pro. Substrates are oligopeptides, preferentially tripeptides =

Synonyms

brain dipeptidyl aminopeptidase A, carboxytripeptidase, dipeptidyl aminopeptidase II, dipeptidyl arylamidase II, dipeptidyl peptidase, dipeptidyl peptidase 2, dipeptidyl peptidase II, dipeptidyl-aminopeptidase II, dipeptidylpeptidase II, DP II, DPP, DPP II, DPP-II, DPP2, DPP7, DPPII, DPPV, fibroblast activation protein alpha, GPA, More, peptidase, dipeptidyl, II, QPP, quiescent cell proline dipeptidase, seprase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.14 Dipeptidyl-peptidases and tripeptidyl-peptidases
                3.4.14.2 dipeptidyl-peptidase II

Purification

Purification on EC 3.4.14.2 - dipeptidyl-peptidase II

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
131.3fold to homogeneity, from kidney
-
ammonium sulfate precipitation and anion exchange chromatography
-
complexed with adenosine deaminase or dipeptidyl peptidase IV, DEAE cellulose column chromatography, DEAE-Sephadex A-50 gel filtration, Sephadex G-25 gel filtration, and Sephadex G-200 gel filtration
-
enzyme form DPP II-S and DPP II-M
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from placenta
His·Bind Resin column chromatography
-
partial, multiple isoforms
-
purification scheme includes an inhibitor-based affinity chromatographic step