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3.4.11.7: glutamyl aminopeptidase

This is an abbreviated version!
For detailed information about glutamyl aminopeptidase, go to the full flat file.

Word Map on EC 3.4.11.7

Reaction

release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide =

Synonyms

acid aminopeptidase, acidic alpha-aminopeptidase, Aminopeptidase, aminopeptidase A, aminopeptidase A ectopeptidase, aminopeptidase, aspartate, aminopeptidase-A, angiotensinase, angiotensinase A, antigen BP-1/6C3 of mouse B lymphocytes, AP A, APA, Asp-AP, aspartate aminopeptidase, aspartyl aminopeptidase, aspartyl-aminopeptidase, BP-1, BP-1/6C3 antigen, BP1/6C3, Ca2+-activated glutamate aminopeptidase, Differentiation antigen gp160, EAP, ENPEP, GAP, GluAP, glutamyl (aspartyl)-specific aminopeptidase A, glutamyl aminopeptidase, glutamyl aminopeptidase (Lactococcus), glutamyl peptidase, glutamyl-aminopeptidase, glutamyl-AP, hAPA, L-alpha-aspartyl(L-alpha-glutamyl)-peptide hydrolase, L-aspartate aminopeptidase, LAP-A, Lb-PepA, Lc-PepA, leucine aminopeptidase A, M18 aspartyl aminopeptidase, mAPA, membrane aminopeptidase II, membrane-bound aspartyl-AP, MHJ_0125, More, N-terminal acidic amino acid-specific aminopeptidase, PepA, tricorn interacting factor F3, tricorn protease-interacting factor F3, type II Zn2+ membrane-bound aminopeptidase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.7 glutamyl aminopeptidase

Purification

Purification on EC 3.4.11.7 - glutamyl aminopeptidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, anion exchange chromatography, hydrophobic interaction chromatography, gel filtration
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by affinity chromatography on a Ni-NTA column
DEAE Toyopearl column chromatography
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Gloydius blomhoffi brevicaudus venom is centrifuged and the resulting supernatant is subjected to gel-filtration on a Sephacryl S-300 column
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immobilized metal ion affinity chromatography, after tag removal anion exchange chromatography
native enzyme 634fold and 144fold from strain NCDO 607, 727fold from strain NCDO 712, and 387fold from strain NCDO1968
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partial
preparation with 10% leucyl-beta-naphthylaminidase
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recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by dialysis, nickel affinity chromatography, and desalting gel filtration
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant untagged enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, and gel filtration to about 95% purity, method optimization
recombinant enzyme
recombinant enzyme from COS-7 cells by BP-1 antibody affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from CHO-K1 cells by Co2+ affinity chromatography
recombinant soluble enzyme His-tagged from Sf9 insect cells to homogeneity by ultrafiltration, dialysis, anion exchange and cobalt affinity chromatography
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Talon Co2+ column chromatography
two isoforms: ATA I and ATA II
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using a Co2+-charged chelating Toyopearl column
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