3.4.11.21: aspartyl aminopeptidase
This is an abbreviated version!
For detailed information about aspartyl aminopeptidase, go to the full flat file.
Reaction
release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate
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Synonyms
AAP, acid aminopeptidase, acid peptidase, alpha-aspartyl dipeptidase, Aminopeptidase, aminopeptidase A, angiotensinase, APA, Ape4, Ape4 aspartyl aminopeptidase, Ape4 metalloprotease, Asp-AP, AspAP, aspartate aminopeptidase, aspartic aminopeptidase, aspartyl aminopeptidase, aspartyl(glutamyl)-specific aminopeptidase, aspartyl-AP, CNAG_01169, DAP, DNPEP, EC 3.4.11.7, glutamyl (aspartyl)-specific aminopeptidase A, glutamyl aminopeptidase, L-aspartate aminopeptidase, Lb-PepA, Lc-PepA, M18 aspartyl aminopeptidase, M18AAP, More, PepA, peptidase E, PfM18AAP, PvM18AAP, soluble acid aminopeptidase, TgAAP, TGGT1_297970, Yhr113w
ECTree
Engineering
Engineering on EC 3.4.11.21 - aspartyl aminopeptidase
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H170F
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inactive mutant enzyme
H33F
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mutant enzyme with decreased turnover number
H349F
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mutant enzyme with decreased turnover number
H352F
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dramatically reduced activity, destabilization of quarternary structure and dissociation of the native 440000 Da enzyme
H359F
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mutant enzyme with decreased turnover number
H363F
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mutant enzyme with decreased turnover number
H440F
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inactive mutant enzyme
H94F
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inactive mutant enzyme
additional information
random insertional mutagenesis, the ape4 mutants are sensitive to high temperature growth. ape4 Mutation affects multiple virulence factors in Cryptococcus neoformans
additional information
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random insertional mutagenesis, the ape4 mutants are sensitive to high temperature growth. ape4 Mutation affects multiple virulence factors in Cryptococcus neoformans
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additional information
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random insertional mutagenesis, the ape4 mutants are sensitive to high temperature growth. ape4 Mutation affects multiple virulence factors in Cryptococcus neoformans
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additional information
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random insertional mutagenesis, the ape4 mutants are sensitive to high temperature growth. ape4 Mutation affects multiple virulence factors in Cryptococcus neoformans
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additional information
knockout of enzyme TgAAP using the CRISPR/Cas9 knockout system, knockout plasmid pCD-TgAAP is constructed by inserting DHFR coding sequence and TgAAP-specific single guide RNA (gRNA) into plasmid pSAG1::CAS9-U6::sgUPRT, which contains Cas9, nuclear localization signal (NLS), and expresses GFP fusion element. The substrate activity is almost completely lost in the DELTATgAAP strain, thus, no other enzyme complements the TgAAP activity
additional information
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knockout of enzyme TgAAP using the CRISPR/Cas9 knockout system, knockout plasmid pCD-TgAAP is constructed by inserting DHFR coding sequence and TgAAP-specific single guide RNA (gRNA) into plasmid pSAG1::CAS9-U6::sgUPRT, which contains Cas9, nuclear localization signal (NLS), and expresses GFP fusion element. The substrate activity is almost completely lost in the DELTATgAAP strain, thus, no other enzyme complements the TgAAP activity
additional information
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knockout of enzyme TgAAP using the CRISPR/Cas9 knockout system, knockout plasmid pCD-TgAAP is constructed by inserting DHFR coding sequence and TgAAP-specific single guide RNA (gRNA) into plasmid pSAG1::CAS9-U6::sgUPRT, which contains Cas9, nuclear localization signal (NLS), and expresses GFP fusion element. The substrate activity is almost completely lost in the DELTATgAAP strain, thus, no other enzyme complements the TgAAP activity
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additional information
-
knockout of enzyme TgAAP using the CRISPR/Cas9 knockout system, knockout plasmid pCD-TgAAP is constructed by inserting DHFR coding sequence and TgAAP-specific single guide RNA (gRNA) into plasmid pSAG1::CAS9-U6::sgUPRT, which contains Cas9, nuclear localization signal (NLS), and expresses GFP fusion element. The substrate activity is almost completely lost in the DELTATgAAP strain, thus, no other enzyme complements the TgAAP activity
-
additional information
-
knockout of enzyme TgAAP using the CRISPR/Cas9 knockout system, knockout plasmid pCD-TgAAP is constructed by inserting DHFR coding sequence and TgAAP-specific single guide RNA (gRNA) into plasmid pSAG1::CAS9-U6::sgUPRT, which contains Cas9, nuclear localization signal (NLS), and expresses GFP fusion element. The substrate activity is almost completely lost in the DELTATgAAP strain, thus, no other enzyme complements the TgAAP activity
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